公开(公告)号：US10386301B2

公开(公告)日：2019-08-20

申请号：US15955567

申请日：2018-04-17

Applicant: LI-COR, Inc.

Inventor： Han-Wei Wang ,  Lyle R. Middendorf

IPC: G01N21/64 ,  G06T1/00 ,  G01N21/17

Abstract: Methods and devices are disclosed for the imaging of a biological sample with a top-down camera and a side-view camera. A biological sample is held on an imaging stage that is capable of rotation in two orthogonal axes. The top-down and side-view cameras can record a series of images of the sample using multiple imaging modalities at different rotational positions of the imaging stage. The top-down camera can be translated along its optical axis to affect the camera zoom and influence the resolution and field of view of the recorded images. Fluorescence excitation light sources can be positioned proximate to each of the top-down and side-view cameras to provide substantially uniform illumination of the sample for imaging with each camera.

公开(公告)号：US20180140197A1

公开(公告)日：2018-05-24

申请号：US15819603

申请日：2017-11-21

Applicant: LI-COR, Inc.

Inventor： Han-Wei Wang ,  Lyle R. Middendorf

IPC: A61B5/00 ,  H04N5/232 ,  H04N5/235 ,  A61B6/00

CPC classification number: A61B5/0077 ,  A61B6/481 ,  A61B6/50 ,  G06T1/0007 ,  H04N5/23216 ,  H04N5/23245 ,  H04N5/23254 ,  H04N5/23267 ,  H04N5/2354

Abstract: Methods and systems are provided for the detecting and presenting of faint signals from a real-time interactive imager. A real-time view of a subject is controlled by a user by entering commands that move the subject or a camera used to record the real-time view. The rate of this movement is monitored, and when the rate descends below a preset value, a trigger is sent to the camera. In response to the trigger, the camera switches to a lower frame frequency for capturing the real-time view of the subject, thereby increasing the amount of light collected in each frame, and increasing the sensitivity of the camera to faint signals. The methods and systems are particularly relevant for surgical or biopsy imaging, in which both a higher frame rate preview image of a subject for 3D visualization, and a higher sensitivity fluorescence image of the subject for tumor margin assessment, are desired.