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公开(公告)号：US10993622B2
公开(公告)日：2021-05-04
申请号：US15819603
申请日：2017-11-21
Applicant: LI-COR, Inc.
Inventor： Han-Wei Wang , Lyle R. Middendorf
Abstract: Methods and systems are provided for the detecting and presenting of faint signals from a real-time interactive imager. A real-time view of a subject is controlled by a user by entering commands that move the subject or a camera used to record the real-time view. The rate of this movement is monitored, and when the rate descends below a preset value, a trigger is sent to the camera. In response to the trigger, the camera switches to a lower frame frequency for capturing the real-time view of the subject, thereby increasing the amount of light collected in each frame, and increasing the sensitivity of the camera to faint signals. The methods and systems are particularly relevant for surgical or biopsy imaging, in which both a higher frame rate preview image of a subject for 3D visualization, and a higher sensitivity fluorescence image of the subject for tumor margin assessment, are desired.
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公开(公告)号：US20190323966A1
公开(公告)日：2019-10-24
申请号：US16456511
申请日：2019-06-28
Applicant: LI-COR, Inc.
Inventor： Han-Wei Wang , Lyle R. Middendorf
Abstract: Methods and devices are disclosed for the imaging of a biological sample with a top-down camera and a side-view camera. A biological sample is held on an imaging stage that is capable of rotation in two orthogonal axes. The top-down and side-view cameras can record a series of images of the sample using multiple imaging modalities at different rotational positions of the imaging stage. The top-down camera can be translated along its optical axis to affect the camera zoom and influence the resolution and field of view of the recorded images. Fluorescence excitation light sources can be positioned proximate to each of the top-down and side-view cameras to provide substantially uniform illumination of the sample for imaging with each camera.
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公开(公告)号：US20180306720A1
公开(公告)日：2018-10-25
申请号：US15955567
申请日：2018-04-17
Applicant: LI-COR, Inc.
Inventor： Han-Wei Wang , Lyle R. Middendorf
IPC: G01N21/64
CPC classification number: G01N21/6458 , G01N21/6428 , G01N2021/177 , G01N2021/1787 , G01N2021/6439 , G06T1/0007
Abstract: Methods and devices are disclosed for the imaging of a biological sample with a top-down camera and a side-view camera. A biological sample is held on an imaging stage that is capable of rotation in two orthogonal axes. The top-down and side-view cameras can record a series of images of the sample using multiple imaging modalities at different rotational positions of the imaging stage. The top-down camera can be translated along its optical axis to affect the camera zoom and influence the resolution and field of view of the recorded images. Fluorescence excitation light sources can be positioned proximate to each of the top-down and side-view cameras to provide substantially uniform illumination of the sample for imaging with each camera.
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公开(公告)号：US20210341389A1
公开(公告)日：2021-11-04
申请号：US16865044
申请日：2020-05-01
Applicant: LI-COR, Inc.
Inventor： Han-Wei Wang , Lyle R. Middendorf
IPC: G01N21/64
Abstract: An imager for simultaneously taking full color pictures and fluorescence images from the same perspectives, top-down and side views, is presented that uses multiple cameras looking at the same stage through a beamsplitter and light sources for each view. The imager is configured to work with a particular fluorophore, or at least a predetermined fluorescence excitation wavelength and emission wavelength. A broadband white light source projects through a shortpass or other filter with a cut-off wavelength either lower than or between the excitation and emission wavelengths. Another shortpass or other filter is in front of a color camera with a cut-off wavelength below that of the emission wavelength, while a monochrome fluorescence camera may or may not have additional filters to bring out the fluorescence. These filters may be built into a dichroic mirror of the beamsplitter. In addition, digital processing may boost frequencies in the color image that were dampened by the filters.
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公开(公告)号：US11154862B2
公开(公告)日：2021-10-26
申请号：US16876895
申请日：2020-05-18
Applicant: LI-COR, Inc.
Inventor： Michael D. Furtaw , Donald T. Lamb , Lyle R. Middendorf
IPC: G01N27/447 , B01L3/00 , B01L3/02 , G01N1/40
Abstract: Devices and methods are provided for the separation and dispensing of material using a microfluidic separation column connected via an exit channel to one or more sheath flow channels. The flow of separated material through the separation column is at least partially driven by a voltage potential between a first electrode within the separation column and a terminating electrode within at least one of the sheath flow channels. The separation column, exit channel, sheath flow channels, and electrodes are all within a single monolithic chip. The presence of an on-chip terminating electrode allows for separated material to be entrained in the sheath fluids and ejected onto a surface that can be non-conductive. The presence of multiple sheath flows allows for sheath flow fluids to have different compositions from one another, while reducing the occurrence of sheath flow fluids entering the separation column.
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Patent Agency Ranking
公开(公告)号：US20210219843A1
公开(公告)日：2021-07-22
申请号：US17213235
申请日：2021-03-26
Applicant: LI-COR, Inc.
Inventor： Han-Wei Wang , Lyle R. Middendorf
Abstract: Methods and systems are provided for the detecting and presenting of faint signals from a real-time interactive imager. A real-time view of a subject is controlled by a user by entering commands that move the subject or a camera used to record the real-time view. The rate of this movement is monitored, and when the rate descends below a preset value, a trigger is sent to the camera. In response to the trigger, the camera switches to a lower frame frequency for capturing the real-time view of the subject, thereby increasing the amount of light collected in each frame, and increasing the sensitivity of the camera to faint signals. The methods and systems are particularly relevant for surgical or biopsy imaging, in which both a higher frame rate preview image of a subject for 3D visualization, and a higher sensitivity fluorescence image of the subject for tumor margin assessment, are desired.
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公开(公告)号：US20180036730A1
公开(公告)日：2018-02-08
申请号：US15670939
申请日：2017-08-07
Applicant: LI-COR, Inc.
Inventor： Michael D. Furtaw , Donald T. Lamb , Lyle R. Middendorf
IPC: B01L3/00 , G01N27/447 , G01N1/40
Abstract: Devices and methods are provided for the separation and dispensing of material using a microfluidic separation column connected via an exit channel to one or more sheath flow channels. The flow of separated material through the separation column is at least partially driven by a voltage potential between a first electrode within the separation column and a terminating electrode within at least one of the sheath flow channels. The separation column, exit channel, sheath flow channels, and electrodes are all within a single monolithic chip. The presence of an on-chip terminating electrode allows for separated material to be entrained in the sheath fluids and ejected onto a surface that can be non-conductive. The presence of multiple sheath flows allows for sheath flow fluids to have different compositions from one another, while reducing the occurrence of sheath flow fluids entering the separation column.
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公开(公告)号：US10775309B2
公开(公告)日：2020-09-15
申请号：US16456511
申请日：2019-06-28
Applicant: LI-COR, Inc.
Inventor： Han-Wei Wang , Lyle R. Middendorf
Abstract: Methods and devices are disclosed for the imaging of a biological sample with a top-down camera and a side-view camera. A biological sample is held on an imaging stage that is capable of rotation in two orthogonal axes. The top-down and side-view cameras can record a series of images of the sample using multiple imaging modalities at different rotational positions of the imaging stage. The top-down camera can be translated along its optical axis to affect the camera zoom and influence the resolution and field of view of the recorded images. Fluorescence excitation light sources can be positioned proximate to each of the top-down and side-view cameras to provide substantially uniform illumination of the sample for imaging with each camera.
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9.发明申请公开(公告)号：US20200276583A1
公开(公告)日：2020-09-03
申请号：US16876895
申请日：2020-05-18
Applicant: LI-COR, Inc.
Inventor： Michael D. Furtaw , Donald T. Lamb , Lyle R. Middendorf
IPC: B01L3/00 , B01L3/02 , G01N27/447 , G01N1/40
Abstract: Devices and methods are provided for the separation and dispensing of material using a microfluidic separation column connected via an exit channel to one or more sheath flow channels. The flow of separated material through the separation column is at least partially driven by a voltage potential between a first electrode within the separation column and a terminating electrode within at least one of the sheath flow channels. The separation column, exit channel, sheath flow channels, and electrodes are all within a single monolithic chip. The presence of an on-chip terminating electrode allows for separated material to be entrained in the sheath fluids and ejected onto a surface that can be non-conductive. The presence of multiple sheath flows allows for sheath flow fluids to have different compositions from one another, while reducing the occurrence of sheath flow fluids entering the separation column.
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公开(公告)号：US10737268B2
公开(公告)日：2020-08-11
申请号：US15670939
申请日：2017-08-07
Applicant: LI-COR, Inc.
Inventor： Michael D. Furtaw , Donald T. Lamb , Lyle R. Middendorf
IPC: G01N27/447 , B01L3/00 , B01L3/02 , G01N1/40
Abstract: Devices and methods are provided for the separation and dispensing of material using a microfluidic separation column connected via an exit channel to one or more sheath flow channels. The flow of separated material through the separation column is at least partially driven by a voltage potential between a first electrode within the separation column and a terminating electrode within at least one of the sheath flow channels. The separation column, exit channel, sheath flow channels, and electrodes are all within a single monolithic chip. The presence of an on-chip terminating electrode allows for separated material to be entrained in the sheath fluids and ejected onto a surface that can be non-conductive. The presence of multiple sheath flows allows for sheath flow fluids to have different compositions from one another, while reducing the occurrence of sheath flow fluids entering the separation column.
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