公开(公告)号：US11952495B1

公开(公告)日：2024-04-09

申请号：US17498377

申请日：2021-10-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yi-Zhen Hu ,  Aimei Chen ,  Daniel Beacham ,  Chrisgen Vonnegut ,  Krishnamurthy Nacharaju

IPC: C09B11/24 ,  G01N21/64

CPC classification number: C09B11/24 ,  G01N21/6428 ,  G01N2021/6439

Abstract: The present disclosure provides for compounds of Formula (I),




its corresponding compounds of Formula (II)





or salts thereof and their use as fluorogenic pH sensors.

公开(公告)号：US20150246146A1

公开(公告)日：2015-09-03

申请号：US14425114

申请日：2013-10-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Brian Agnew ,  Robert Aggeler ,  Hee Chol Kang ,  Aimei Chen ,  Kyle Gee

IPC: A61K51/10 ,  C12P21/06 ,  C12P21/00 ,  G01N33/58 ,  C07K14/435 ,  C07K16/18

CPC classification number: A61K51/10 ,  C07K14/435 ,  C07K16/18 ,  C12P21/005 ,  C12P21/06 ,  G01N33/534 ,  G01N33/582 ,  G01N33/6854

Abstract: Provided herein are methods, compositions and kits for use in the site-specific labeling of glycoproteins comprising a combination of enzyme-mediated incorporation of modified sugars comprising a chemical handle and cycloaddition chemistry with a labeling molecule comprising a reactive group, a metal ion chelator, and/or a fluorophore.

Abstract translation: 本文提供了用于糖蛋白的位点特异性标记的方法，组合物和试剂盒，其包括酶介导的包含化学处理和环加成化学的修饰的糖的组合与包含反应性基团的标记分子，金属离子螯合剂， 和/或荧光团。

公开(公告)号：US20150079622A1

公开(公告)日：2015-03-19

申请号：US14384843

申请日：2013-02-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yi-Zhen Hu ,  Aimei Chen ,  Hee Chol Kang ,  Kyle Gee ,  Bhaskar Mandavilli

IPC: G01N33/52 ,  C07D417/06

CPC classification number: G01N33/84 ,  C07D417/06 ,  C09B23/02 ,  C09B23/04 ,  C09B23/06 ,  G01N33/52

Abstract: Disclosed herein are compounds, compositions, methods, and kits for detecting reactive oxygen species (ROS) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and/or high content imaging. The compounds disclosed herein are novel reduced nucleic acid binding cyanine dyes, which dyes are probes for detecting ROS and measuring oxidative stress in cells either in vitro and/or in vivo. Also described herein are processes for preparing novel reduced dyes, i.e., ROS probes, for use in the disclosed compositions, methods and kits.

Abstract translation: 本文公开了通过常规荧光显微镜，荧光光谱，流式细胞术和/或高含量成像检测活性氧（ROS）的化合物，组合物，方法和试剂盒。 本文公开的化合物是新的还原性核酸结合花青染料，其染料是用于检测ROS的探针，并测定体外和/或体内细胞中的氧化应激。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的新型还原染料即ROS探针的方法。