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公开(公告)号：US09599622B2
公开(公告)日：2017-03-21
申请号：US14402358
申请日：2013-03-14
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Kyle Gee , Upinder Singh , Aleksey Rukavishnikov , Daniel Beacham , Shih-Jung Huang , Michael Janes , Wenjun Zhou
CPC classification number: C09B11/24 , A61K49/0041 , C07D311/90 , G01N33/5005 , G01N33/582 , G01N33/84
Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.
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公开(公告)号：US20150241438A1
公开(公告)日：2015-08-27
申请号：US14402375
申请日：2013-03-14
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Kyle Gee , Upinder Singh , Aleksey Rukavishnikov , Daniel Beacham , Shih-Jung Huang , Michael Janes , Wenjun Zhou , Judith Berlier
IPC: G01N33/58 , C07D311/90
CPC classification number: C09B11/24 , A61K49/0041 , C07D311/90 , G01N33/5005 , G01N33/582 , G01N33/84
Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.
Abstract translation: 本文公开了使用pH敏感荧光染料检测样品中的pH的化合物，组合物，方法和试剂盒。 本文公开的化合物是包含具有一个或多个给电子基团的苯胺部分的新型呫吨衍生物染料，该染料用于在体外或体内检测样品中的pH。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的所述染料的方法。
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公开(公告)号：US20190242912A1
公开(公告)日：2019-08-08
申请号：US16298810
申请日：2019-03-11
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Daniel Beacham , Kyle Gee
CPC classification number: G01N33/84 , C12Q1/02 , G01N33/6872 , G01N2333/705
Abstract: The present invention relates to methods for detecting the activity of an ion channel in a cell. The methods comprise providing a loading buffer solution to a cell that has an ion channel. The loading buffer comprises at least one thallium indicator (e.g., an environmentally sensitive, luminescent dye) and a physiological concentration of chloride ions. The methods further comprise providing a stimulus buffer to the cell, wherein the stimulus buffer comprises thallium (e.g., thallium ions). Providing the stimulus buffer causes thallium influx into the cell through the ion channel. After providing the stimulus buffer, the luminescence (e.g., fluorescence) of the dye in the cell is detected. The luminescence of the dye can change in the presence or absence of thallium. The methods may be used to measure influx or efflux of thallium through an ion channel.
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公开(公告)号：US09910051B2
公开(公告)日：2018-03-06
申请号：US14455550
申请日：2014-08-08
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Daniel Beacham , Jeffrey Dzubay , Kyle Gee , Vladimir Martin , Aleksey Rukavishnikov
IPC: G01N33/84 , C07K1/13 , C09B7/00 , C09B11/12 , C09B11/28 , C09B57/00 , C09B69/00 , G01N31/22 , C09B11/24 , G01N33/50 , G01N33/58 , G01N33/80 , C07D311/82 , C07D311/90 , C07D405/12 , C07D413/04 , C07D491/22 , C07D209/14 , C09K11/06 , C07F5/02
CPC classification number: G01N33/84 , C07D209/14 , C07D311/82 , C07D311/90 , C07D405/12 , C07D413/04 , C07D491/22 , C07F5/02 , C07K1/13 , C09B7/00 , C09B11/12 , C09B11/24 , C09B11/28 , C09B57/00 , C09B69/00 , C09K11/06 , G01N31/22 , G01N33/5005 , G01N33/5058 , G01N33/5091 , G01N33/582 , G01N33/80 , G01N2333/245 , Y10T436/143333
Abstract: A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I: wherein the variables are described throughout the application.
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公开(公告)号：US11566221B2
公开(公告)日：2023-01-31
申请号：US16344497
申请日：2017-10-20
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Soojung Shin , Yiping Yan , Daniel Beacham
IPC: C12N5/0793 , C12N5/00 , C12N5/0735 , C12N5/074
Abstract: Embodiments herein provide methods of differentiating neural stem cells to neuronal cells while concomitantly retarding neural stem cell proliferation. Resultant cultures demonstrate reduced clumping of cells, increased purity of neuronal cells and accelerated electrophysiology as compared to control methods.
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Patent Agency Ranking
公开(公告)号：US20200088676A1
公开(公告)日：2020-03-19
申请号：US16568133
申请日：2019-09-11
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Wolfgang Hinz , John Donohue , Daniel Beacham
IPC: G01N27/414 , G01N27/28 , G01N27/30 , G01N27/407 , C09D189/00
Abstract: Various cell analysis systems of the present teachings can measure the electrical and metabolic activity of single, living cells with subcellular addressability and simultaneous data acquisition for between about 10 cells to about 500,000 cells in a single analysis. Various sensor array devices of the present teachings can have sensor arrays with between 20 million to 660 million ChemFET sensors built into a massively paralleled array and can provide for simultaneous measurement of cells with data acquisition rates in the kilohertz (kHz) range. As various ChemFET sensor arrays of the present teachings can detect chemical analytes as well detect changes in cell membrane potential, various cell analysis systems of the present teachings also provide for the controlled chemical and electrical interrogation of cells.
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公开(公告)号：US20150218379A1
公开(公告)日：2015-08-06
申请号：US14402358
申请日：2013-03-14
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Kyle Gee , Upinder Singh , Aleksey Rukavishnikov , Daniel Beacham , Shih-Jung Huang , Michael Janes , Wenjun Zhou
CPC classification number: C09B11/24 , A61K49/0041 , C07D311/90 , G01N33/5005 , G01N33/582 , G01N33/84
Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.
Abstract translation: 本文公开了使用pH敏感荧光染料检测样品中的pH的化合物，组合物，方法和试剂盒。 本文公开的化合物是包含具有一个或多个给电子基团的苯胺部分的新型呫吨衍生物染料，该染料用于在体外或体内检测样品中的pH。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的所述染料的方法。
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公开(公告)号：US11952495B1
公开(公告)日：2024-04-09
申请号：US17498377
申请日：2021-10-11
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Yi-Zhen Hu , Aimei Chen , Daniel Beacham , Chrisgen Vonnegut , Krishnamurthy Nacharaju
CPC classification number: C09B11/24 , G01N21/6428 , G01N2021/6439
Abstract: The present disclosure provides for compounds of Formula (I),
its corresponding compounds of Formula (II)
or salts thereof and their use as fluorogenic pH sensors.-
公开(公告)号：US11867698B2
公开(公告)日：2024-01-09
申请号：US17027391
申请日：2020-09-21
Applicant: Life Technologies Corporation
Inventor： Jeffrey Dzubay , Kyle Gee , Vladimir Martin , Aleksey Rukavishnikov , Daniel Beacham
IPC: G01N33/58 , G01N33/50 , G01N33/84 , C09B11/24 , C09B11/28 , C07D311/82 , C07D311/90 , C07D405/12 , C07D413/04 , C07D491/22 , C07D209/14 , C09K11/06 , C07F5/02 , C07K1/13 , C09B7/00 , C09B11/12 , C09B57/00 , C09B69/00 , G01N31/22 , G01N33/80
CPC classification number: G01N33/582 , C07D209/14 , C07D311/82 , C07D311/90 , C07D405/12 , C07D413/04 , C07D491/22 , C07F5/02 , C07K1/13 , C09B7/00 , C09B11/12 , C09B11/24 , C09B11/28 , C09B57/00 , C09B69/00 , C09K11/06 , G01N31/22 , G01N33/5005 , G01N33/5058 , G01N33/5091 , G01N33/80 , G01N33/84 , G01N2333/245 , Y10T436/143333
Abstract: A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I:
wherein the variables are described throughout the application.-
公开(公告)号：US10253185B2
公开(公告)日：2019-04-09
申请号：US15423890
申请日：2017-02-03
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Kyle Gee , Upinder Singh , Aleksey Rukavishnikov , Daniel Beacham , Shih-Jung Huang , Michael Janes , Wenjun Zhou
Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.
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