公开(公告)号：US20230122742A1

公开(公告)日：2023-04-20

申请号：US17976128

申请日：2022-10-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Gordon A. JANAWAY ,  Mark ANDERSEN ,  Kornelija ZGONC ,  Michael C. PALLAS ,  Marcin SIKORA ,  Casey R. McFARLAND ,  Ferrier N. LE ,  Haopeng Wang ,  Jian GONG ,  Gothami Padmabandu

IPC: C12Q1/6886 ,  C12Q1/686 ,  G01N21/64

Abstract: Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.

公开(公告)号：US20200224280A1

公开(公告)日：2020-07-16

申请号：US16741299

申请日：2020-01-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Gordon A. JANAWAY ,  Mark ANDERSEN ,  Kornelija ZGONC ,  Michael C. PALLAS ,  Marcin SIKORA ,  Casey R. McFARLAND ,  Ferrier N. LE ,  Haopeng Wang ,  Jian GONG ,  Gothami Padmabandu

IPC: C12Q1/6886 ,  C12Q1/686 ,  G01N21/64

Abstract: Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.

公开(公告)号：US20200043571A1

公开(公告)日：2020-02-06

申请号：US16550374

申请日：2019-08-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Christian KOLLER ,  Marcin SIKORA ,  Peter VANDER HORN

IPC: G16B40/10 ,  G16B5/00 ,  G16B20/00 ,  G16B25/00 ,  G16B30/00 ,  C12Q1/6869

Abstract: A method for nucleic acid sequencing includes receiving observed or measured nucleic acid sequencing data from a sequencing instrument that receives and processes a sample nucleic acid in a termination sequencing-by-synthesis process. The method also includes generating a set of candidate sequences of bases for the observed or measured nucleic acid sequencing data by determining a predicted signal for candidate sequences using a simulation framework. The simulation framework incorporates an estimated carry forward rate (CFR), an estimated incomplete extension rate (IER), an estimated droop rate (DR), an estimated reactivated molecules rate (RMR), and an estimated termination failure rate (TFR), the RMR being greater than or equal to zero and the TFR being lesser than one. The method also includes identifying, from the set of candidate sequences of bases, one candidate sequence leading to optimization of a solver function as corresponding to the sequence for the sample nucleic acid.