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    COMPOSITIONS, METHODS, SYSTEMS AND KITS FOR TARGET NUCLEIC ACID ENRICHMENT
    1.
    发明申请
    COMPOSITIONS, METHODS, SYSTEMS AND KITS FOR TARGET NUCLEIC ACID ENRICHMENT 审中-公开

    公开(公告)号：US20200239936A1

    公开(公告)日：2020-07-30

    申请号：US16846932

    申请日：2020-04-13

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Mark ANDERSEN ,  Steven ROMAN

    IPC: C12Q1/6806 ,  C12Q1/6853 ,  C07H21/00

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the concentration of target nucleic acid molecules from a sample. In one aspect, the invention relates to purifying a primer extension product from a primer extension reaction mixture. In some aspects, nucleic acid molecules obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing.

    LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR
    3.
    发明申请
    LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR 审中-公开

    公开(公告)号：US20190002872A1

    公开(公告)日：2019-01-03

    申请号：US16024175

    申请日：2018-06-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Mark ANDERSEN ,  Daniel MAZUR

    IPC: C12N15/10 ,  C12Q1/6855

    Abstract: Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.

    MULTIPLEX TRANSCRIPTOME ANALYSIS
    5.
    发明申请
    MULTIPLEX TRANSCRIPTOME ANALYSIS 审中-公开

    公开(公告)号：US20160194694A1

    公开(公告)日：2016-07-07

    申请号：US15068466

    申请日：2016-03-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Kelli BRAMLETT ,  John LEAMON ,  Mark ANDERSEN ,  Michael THORNTON

    IPC: C12Q1/68

    CPC classification number: C12Q1/6855 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2525/117

    Abstract: In some embodiments, the disclosure relates generally to methods, compositions, systems, apparatuses and kits comprising a multiplex nucleic acid amplification reaction that employs a plurality (e.g., hundreds, thousands, tens-of-thousands or hundreds-of-thousands) of different target-specific primer pairs that enable substantially simultaneous amplification of a plurality of different target sequences-of-interest in a single reaction mixture. In some embodiments, the multiplex nucleic acid amplification reaction generates a plurality of amplicons having sequences derived from a sample containing RNA or DNA, including whole transcriptome or genomic samples. In some embodiments, the sequences and abundances of at least some of the plurality of amplicons are characterized, optionally simultaneously or through a single assay, by suitable detection methods, including sequencing or other procedures known in the art.

    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR
    6.
    发明申请
    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR 审中-公开

    公开(公告)号：US20130059762A1

    公开(公告)日：2013-03-07

    申请号：US13679706

    申请日：2012-11-16

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： John LEAMON ,  Mark ANDERSEN ,  Michael THORNTON

    IPC: C40B50/06

    CPC classification number: C12Q1/6855 ,  C12Q1/6806 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR
    7.
    发明申请
    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR 有权

    公开(公告)号：US20210032679A1

    公开(公告)日：2021-02-04

    申请号：US16949159

    申请日：2020-10-15

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： John LEAMON ,  Mark ANDERSEN ,  Michael THORNTON

    IPC: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12N15/10 ,  C12Q1/6886 ,  C12Q1/6874

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

    MULTIPLEX TRANSCRIPTOME ANALYSIS
    9.
    发明申请
    MULTIPLEX TRANSCRIPTOME ANALYSIS 审中-公开
    多重代码分析

    公开(公告)号：US20150344938A1

    公开(公告)日：2015-12-03

    申请号：US14826385

    申请日：2015-08-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Kelli BRAMLETT ,  John LEAMON ,  Mark ANDERSEN ,  Michael THORNTON

    IPC: C12Q1/68

    CPC classification number: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2525/117

    Abstract: In some embodiments, the disclosure relates generally to methods, compositions, systems, apparatuses and kits comprising a multiplex nucleic acid amplification reaction that employs a plurality (e.g., hundreds, thousands, tens-of-thousands or hundreds-of-thousands) of different target-specific primer pairs that enable substantially simultaneous amplification of a plurality of different target sequences-of-interest in a single reaction mixture. In some embodiments, the multiplex nucleic acid amplification reaction generates a plurality of amplicons having sequences derived from a sample containing RNA or DNA, including whole transcriptome or genomic samples. In some embodiments, the sequences and abundances of at least some of the plurality of amplicons are characterized, optionally simultaneously or through a single assay, by suitable detection methods, including sequencing or other procedures known in the art.

    Abstract translation: 在一些实施方案中，本公开一般涉及包含多重核酸扩增反应的方法，组合物，系统，装置和试剂盒，所述多重核酸扩增反应采用多个（例如，数百，数万，数万或数十万）不同的 靶特异性引物对，其能够在单个反应混合物中基本上同时扩增感兴趣的多个不同靶序列。 在一些实施方案中，多重核酸扩增反应产生具有衍生自含有RNA或DNA的样品的序列的多个扩增子，包括全转录组或基因组样品。 在一些实施方案中，通过合适的检测方法，包括测序或本领域已知的其它程序，可以任选地同时或通过单个测定来表征多个扩增子中的至少一些的序列和丰度。

    Flat-Field Imaging System and Methods of Use
    10.
    发明申请
    Flat-Field Imaging System and Methods of Use 有权

    公开(公告)号：US20220065767A1

    公开(公告)日：2022-03-03

    申请号：US17525477

    申请日：2021-11-12

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Mark ANDERSEN ,  Michael PALLAS ,  Haopeng Wang

    IPC: G01N15/04 ,  C12Q1/6816 ,  C12Q1/686

    Abstract: A method of aligning a plurality of targets is provided. The method includes generating a plurality of targets. A third phase includes the plurality of targets. The method further includes combining a first phase, a second phase, and the third phase in a volume. The first phase, the second phase, and the third phase are substantially immiscible, and the third phase is in fluid communication with the first phase and the second phase, and the first phase, the second phase, and the third phase are operable to be in a configuration of the third phase between the first phase and the second phase in the volume.

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