公开(公告)号：US11255813B2

公开(公告)日：2022-02-22

申请号：US16271979

申请日：2019-02-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Melville Davey ,  Simon Cawley ,  Alan Williams

IPC: G01N27/414 ,  G16B30/00 ,  G16B30/10 ,  G16B30/20 ,  G01N27/27

Abstract: A method for nucleic acid sequencing includes receiving a plurality of signals indicative of a parameter measured for a plurality of defined spaces, at least some of the defined spaces including one or more sample nucleic acids, the signals being responsive to a plurality of nucleotide flows introducing nucleotides to the defined spaces; determining, for at least some of the defined spaces, whether the defined space includes a sample nucleic acid; processing, for at least some of the defined spaces determined to include a sample nucleic acid, the received signals to improve a quality of the received signals; and predicting a plurality of nucleotide sequences corresponding to respective sample nucleic acids for the defined spaces based on the processed signals and the nucleotide flows.

公开(公告)号：US10273540B2

公开(公告)日：2019-04-30

申请号：US13967665

申请日：2013-08-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Melville Davey ,  Michael Meyer ,  Marcin Sikora ,  Simon Cawley ,  Kirk Pastorian

IPC: G06F19/10 ,  C12Q1/6874

Abstract: A method of estimating a parameter related to sequencing of a sample nucleic acid template includes: receiving signal data relating to nucleotide incorporation events resulting from a series of flows of nucleotides onto an array of wells including (i) a first well containing the sample nucleic acid template and (ii) a plurality of other sample-containing wells; determining sequence information for the sample nucleic acid template using signal data from the first well; and constructing a phase-state model for a set of nucleotide flows that contributed at least in part to the sequence information, wherein the model includes a signal correction parameter that is determined using signal data from the plurality of other sample-containing wells.