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    Predictive Model for Use in Sequencing-by-Synthesis
    3.
    发明申请
    Predictive Model for Use in Sequencing-by-Synthesis 审中-公开
    用于按顺序排序的预测模型

    公开(公告)号：US20140222399A1

    公开(公告)日：2014-08-07

    申请号：US14150855

    申请日：2014-01-09

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Melville Davey ,  Michael Meyer

    IPC: G06F19/12

    CPC classification number: G16B5/00 ,  C12Q1/6874 ,  C12Q2537/165

    Abstract: A method of obtaining a more accurate estimate of a signal correction parameter(s) in sequencing-by-synthesis operations, such as incomplete extension rates, carry forward rates, and/or signal droop rates. The sequencing operation produces signal data. A model is constructed to simulate a population of template strands as it undergoes the sequencing process and becomes divided into different phase-states as the sequencing-by-synthesis progresses. For example, the model may be a phase-state model. The output from the model is used to adjust the signal correction parameter(s). For example, the model may be fitted to the signal data. This fitting results in a more accurate estimate of the signal correction parameter(s). In another embodiment, the signal droop rate is modeled as a decaying function and this decaying function is fitted to the signal data to obtain an improved estimate of the signal droop rate.

    Abstract translation: 一种在逐个合成操作中获得信号校正参数的更精确估计的方法，例如不完全扩展速率，结转率和/或信号下降率。 排序操作产生信号数据。 构建模型以模拟模板链的群体，因为其经历了测序过程，并且随着逐个序列合成的进行而分为不同的相态。 例如，模型可以是阶段状态模型。 模型的输出用于调整信号校正参数。 例如，该模型可以适合于信号数据。 该拟合导致信号校正参数的更精确的估计。 在另一个实施例中，信号下降率被建模为衰减函数，并且该衰减函数适合于信号数据以获得信号下降率的改进估计。

    Methods and apparatus for measuring analytes
    4.
    发明授权
    Methods and apparatus for measuring analytes 有权

    公开(公告)号：US11768171B2

    公开(公告)日：2023-09-26

    申请号：US17063621

    申请日：2020-10-05

    Applicant: Life Technologies Corporation

    Inventor： Todd Rearick ,  Melville Davey ,  Mark Beauchemin

    IPC: G01N27/416 ,  G16B30/00 ,  G01N27/414

    CPC classification number: G01N27/4165 ,  G01N27/4145 ,  G16B30/00

    Abstract: A method, computer program product, and system are provided to calibrate a sensor array with a plurality of sensors. The method can include sweeping a voltage of a reference electrode from a first voltage to a second voltage, where the reference electrode is in fluid communication with the sensor array. The output voltage of each of the plurality of sensors can be monitored at one or more voltages within the first and second voltages. An overall average gain of the plurality of sensors can be calculated at each of the one or more voltages. Further, an acquisition window for the sensor array can be determined. The acquisition window can include a maximum distribution of sensors that provides a maximal overall average gain at a particular reference electrode voltage.

    METHODS, SYSTEMS, AND COMPUTER READABLE MEDIA FOR MAKING BASE CALLS IN NUCLEIC ACID SEQUENCING
    5.
    发明申请
    METHODS, SYSTEMS, AND COMPUTER READABLE MEDIA FOR MAKING BASE CALLS IN NUCLEIC ACID SEQUENCING 审中-公开

    公开(公告)号：US20190170680A1

    公开(公告)日：2019-06-06

    申请号：US16196502

    申请日：2018-11-20

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Marcin Sikora ,  Melville Davey ,  Christian Koller ,  Simon Cawley ,  Alan Williams ,  David Kulp

    IPC: G01N27/414 ,  G16B30/00

    Abstract: A method for nucleic acid sequencing includes receiving a plurality of observed or measured signals indicative of a parameter observed or measured for a plurality of defined spaces; determining, for at least some of the defined spaces, whether the defined space comprises one or more sample nucleic acids; processing, for at least some of the defined spaces, the observed or measured signal to improve a quality of the observed or measured signal; generating, for at least some of the defined spaces, a set of candidate sequences of bases for the defined space using one or more metrics adapted to associate a score or penalty to the candidate sequences of bases; and selecting the candidate sequence leading to a highest score or a lowest penalty as corresponding to the correct sequence for the one or more sample nucleic acids in the defined space.

    Methods and apparatus for measuring analytes
    6.
    发明授权
    Methods and apparatus for measuring analytes 有权

    公开(公告)号：US10809226B2

    公开(公告)日：2020-10-20

    申请号：US15920387

    申请日：2018-03-13

    Applicant: Life Technologies Corporation

    Inventor： Todd Rearick ,  Melville Davey ,  Mark Beauchemin

    IPC: G01N27/416 ,  G16B30/00 ,  G01N27/414

    Abstract: A method, computer program product, and system are provided to calibrate a sensor array with a plurality of sensors. The method can include sweeping a voltage of a reference electrode from a first voltage to a second voltage, where the reference electrode is in fluid communication with the sensor array. The output voltage of each of the plurality of sensors can be monitored at one or more voltages within the first and second voltages. An overall average gain of the plurality of sensors can be calculated at each of the one or more voltages. Further, an acquisition window for the sensor array can be determined. The acquisition window can include a maximum distribution of sensors that provides a maximal overall average gain at a particular reference electrode voltage.

    Methods and apparatus for measuring analytes
    7.
    发明授权
    Methods and apparatus for measuring analytes 有权

    公开(公告)号：US09927393B2

    公开(公告)日：2018-03-27

    申请号：US14330756

    申请日：2014-07-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Todd Rearick ,  Melville Davey ,  Mark Beauchemin

    IPC: G01N27/416 ,  G01N27/414 ,  G06F19/22

    CPC classification number: G01N27/4165 ,  G01N27/4145 ,  G06F19/22

    Abstract: A method, computer program product, and system are provided to calibrate a sensor array with a plurality of sensors. The method can include sweeping a voltage of a reference electrode from a first voltage to a second voltage, where the reference electrode is in fluid communication with the sensor array. The output voltage of each of the plurality of sensors can be monitored at one or more voltages within the first and second voltages. An overall average gain of the plurality of sensors can be calculated at each of the one or more voltages. Further, an acquisition window for the sensor may can be determined. The acquisition window can include a maximum distribution of sensors that provides a maximal overall average gain at a particular reference electrode voltage.

    METHODS, SYSTEMS, AND COMPUTER READABLE MEDIA FOR NUCLEIC ACID SEQUENCING
    10.
    发明申请
    METHODS, SYSTEMS, AND COMPUTER READABLE MEDIA FOR NUCLEIC ACID SEQUENCING 有权

    公开(公告)号：US20220283117A1

    公开(公告)日：2022-09-08

    申请号：US17650941

    申请日：2022-02-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Melville Davey ,  Simon Cawley ,  Alan Williams

    IPC: G01N27/414 ,  G16B30/00 ,  G16B30/10 ,  G16B30/20

    Abstract: A method for nucleic acid sequencing includes receiving a plurality of signals indicative of a parameter measured for a plurality of defined spaces, at least some of the defined spaces including one or more sample nucleic acids, the signals being responsive to a plurality of nucleotide flows introducing nucleotides to the defined spaces; determining, for at least some of the defined spaces, whether the defined space includes a sample nucleic acid; processing, for at least some of the defined spaces determined to include a sample nucleic acid, the received signals to improve a quality of the received signals; and predicting a plurality of nucleotide sequences corresponding to respective sample nucleic acids for the defined spaces based on the processed signals and the nucleotide flows.

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