公开(公告)号：US11255813B2

公开(公告)日：2022-02-22

申请号：US16271979

申请日：2019-02-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Melville Davey ,  Simon Cawley ,  Alan Williams

IPC: G01N27/414 ,  G16B30/00 ,  G16B30/10 ,  G16B30/20 ,  G01N27/27

Abstract: A method for nucleic acid sequencing includes receiving a plurality of signals indicative of a parameter measured for a plurality of defined spaces, at least some of the defined spaces including one or more sample nucleic acids, the signals being responsive to a plurality of nucleotide flows introducing nucleotides to the defined spaces; determining, for at least some of the defined spaces, whether the defined space includes a sample nucleic acid; processing, for at least some of the defined spaces determined to include a sample nucleic acid, the received signals to improve a quality of the received signals; and predicting a plurality of nucleotide sequences corresponding to respective sample nucleic acids for the defined spaces based on the processed signals and the nucleotide flows.

公开(公告)号：US10273540B2

公开(公告)日：2019-04-30

申请号：US13967665

申请日：2013-08-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Melville Davey ,  Michael Meyer ,  Marcin Sikora ,  Simon Cawley ,  Kirk Pastorian

IPC: G06F19/10 ,  C12Q1/6874

Abstract: A method of estimating a parameter related to sequencing of a sample nucleic acid template includes: receiving signal data relating to nucleotide incorporation events resulting from a series of flows of nucleotides onto an array of wells including (i) a first well containing the sample nucleic acid template and (ii) a plurality of other sample-containing wells; determining sequence information for the sample nucleic acid template using signal data from the first well; and constructing a phase-state model for a set of nucleotide flows that contributed at least in part to the sequence information, wherein the model includes a signal correction parameter that is determined using signal data from the plurality of other sample-containing wells.

公开(公告)号：US20140093881A1

公开(公告)日：2014-04-03

申请号：US14018069

申请日：2013-09-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Charles Sugnet ,  Simon Cawley ,  Mohit Gupta ,  Iztok Marjanovic

IPC: G06F19/00

CPC classification number: G06F19/702 ,  G06F19/709 ,  H03M7/30

Abstract: A compression method includes: measuring a waveform associated with a chemical event occurring on a sensor array, wherein the waveform comprises a plurality of measured values and the chemical event is indicative of a number of nucleotide incorporations in a genetic sequencing reaction; applying a first compression process to the waveform, the first compression process including a truncating of data corresponding to a portion of the waveform that is not related to nucleotide incorporations in the genetic sequencing reaction; and applying a second compression process to the waveform, the second compression process including a data substitution process that replaces at least a portion of the waveform with a plurality of coefficients representative of the portion of the waveform.

Abstract translation: 压缩方法包括：测量与传感器阵列上发生的化学事件相关联的波形，其中所述波形包括多个测量值，并且所述化学事件指示遗传测序反应中的核苷酸结合数量; 对波形应用第一压缩过程，第一压缩过程包括对应于与遗传测序反应中的核苷酸掺入无关的一部分波形的数据截断; 以及对所述波形应用第二压缩处理，所述第二压缩处理包括用代表所述波形部分的多个系数代替所述波形的至少一部分的数据替换处理。