公开(公告)号：US10403000B2

公开(公告)日：2019-09-03

申请号：US15899308

申请日：2018-02-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yong Chu ,  Jeffrey Marks

IPC: G06K9/00 ,  G06T5/00 ,  G06T7/00 ,  G06T7/11 ,  G06T7/73 ,  C12Q1/686 ,  G06T7/136 ,  G06T7/194

Abstract: A method for analyzing biological reaction systems is provided. The method includes receiving an image of a substrate including a plurality of reaction sites after a biological reaction has taken place. Next, the method includes removing a noise background from the first image. The method includes determining an initial position of each reaction site based on an intensity threshold to generate a initial position set, then refining the initial position set of each reaction site based on an expected pattern of locations of the plurality of reaction sites to generate a first refined position set. The method further includes determining a presence or absence of a fluorescent emission from each reaction site based on the first refined position set and the first image.

公开(公告)号：US20180292320A1

公开(公告)日：2018-10-11

申请号：US16010359

申请日：2018-06-15

Applicant: Life Technologies Corporation

Inventor： Yong Chu ,  Jeffrey Marks ,  Jacob Freudenthal ,  Thomas Wessel ,  David Woo

IPC: G01N21/64 ,  C12Q1/686 ,  G01N21/27 ,  C12Q1/6851

Abstract: In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging fluorescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration to determine reaction site positions in an image. The method further includes performing a pure dye calibration to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration to determine a filter normalization factor. The method includes performing an RNase P validation to validate the instrument is capable of distinguishing between two different quantities of sample.

公开(公告)号：US09898820B2

公开(公告)日：2018-02-20

申请号：US14778415

申请日：2014-03-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yong Chu ,  Jeffrey Marks

IPC: G06K9/00 ,  G06T7/00 ,  G06T5/00 ,  C12Q1/68 ,  G06T7/73 ,  G06T7/11 ,  G06T7/194 ,  G06T7/136

CPC classification number: G06T7/74 ,  C12Q1/686 ,  G06K9/00 ,  G06K2209/07 ,  G06T5/002 ,  G06T7/0012 ,  G06T7/11 ,  G06T7/136 ,  G06T7/194 ,  G06T2207/10064 ,  G06T2207/20024 ,  G06T2207/20208 ,  G06T2207/30072

Abstract: A method for analyzing biological reaction systems is provided. The method includes receiving an image of a substrate including a plurality of reaction sites after a biological reaction has taken place. Next, the method includes removing a noise background from the first image. The method includes determining an initial position of each reaction site based on an intensity threshold to generate a initial position set, then refining the initial position set of each reaction site based on an expected pattern of locations of the plurality of reaction sites to generate a first refined position set. The method further includes determining a presence or absence of a fluorescent emission from each reaction site based on the first refined position set and the first image.

公开(公告)号：US11836614B2

公开(公告)日：2023-12-05

申请号：US16555803

申请日：2019-08-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yong Chu ,  Nivedita Majumbar ,  Manjula Aliminati ,  Yongzhi Chen ,  Kevin Papenfuss

IPC: G06N3/08 ,  C12Q1/686 ,  G06V10/82 ,  G06N3/044 ,  G06N3/045 ,  G06N3/02

CPC classification number: G06N3/08 ,  C12Q1/686 ,  G06N3/044 ,  G06N3/045 ,  G06V10/82 ,  G06N3/02

Abstract: A system and methods are provided for image driven quality control for array based PCR. The system comprises a PCR unit, a reaction array plate, a convolutional neural network (CNN) configured to receive a sequence of images of the reaction array plate in the PCR system, and an output of the CNN coupled to a control for the reaction array plate. The method comprises applying a sequence of images from a plurality of subarrays of the reaction array plate to a plurality of CNNs during operation of the PCR system on the reaction plate array, operating the CNNs to generate failure mode predictions for the reaction plate based on the sequence of images, and coupling an output of the CNNs to one or more of a setting for manufacture of the reaction array plate or to control the PCR system.

公开(公告)号：US20160230209A1

公开(公告)日：2016-08-11

申请号：US15017034

申请日：2016-02-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yong Chu ,  Jacob Freudenthal ,  Jeffrey Marks

IPC: C12Q1/68 ,  G01N21/64

CPC classification number: C12Q1/686 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2201/127

Abstract: Some embodiments describe a computer-implemented method for calibrating a fluorescent dye. The method can comprise imaging a sample holder, loaded into an instrument, at more than one channel. The sample holder can comprise a plurality of reaction sites and more than one dye type, with each dye occupying more than one reaction site. The method can further comprise identifying a peak channel for each dye on the sample holder, normalizing each channel to the peak channel for each dye, and producing a dye matrix that can comprise a set of dye reference values.

Abstract translation: 一些实施例描述了用于校准荧光染料的计算机实现的方法。 该方法可以包括在多个通道上成像加载到仪器中的样品架。 样品架可以包括多个反应位点和多于一种染料类型，每种染料占据多于一个反应位点。 该方法还可以包括鉴定样品架上每种染料的峰通道，将每个通道标准化为每种染料的峰通道，以及产生可包含一组染料参考值的染料基质。