公开(公告)号：US09657332B2

公开(公告)日：2017-05-23

申请号：US14456239

申请日：2014-08-11

Applicant: LUMINEX CORPORATION

Inventor： Doug Whitman ,  Nicolas Arab ,  Chuck Collins

IPC: C12Q1/68 ,  C07H21/00

CPC classification number: C12Q1/6818 ,  C12Q2525/186 ,  C12Q2527/107 ,  C12Q2561/109 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2533/101 ,  C12Q2537/143

Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of primers or probes that comprise a non-natural nucleotide linked to a reporter. Target nucleic acids are detected by the polymerization of a complementary probe or primer that incorporated a cognate non-natural nucleotide linked to a quencher.