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11.发明授权公开(公告)号：US09873746B2
公开(公告)日：2018-01-23
申请号：US13070583
申请日：2011-03-24
CPC分类号： C07K16/2896 , C07K16/00 , C07K16/18 , C07K16/2809 , C07K2317/24 , C12N15/81 , C12N15/815
摘要： Methods are provided for the synthesis and secretion of recombinant proteins preferably large mammalian proteins or hetero-multimeric proteins at high levels and for prolonged time in polyploid, preferably diploid yeast. These methods use various mating competent yeast, including Pichia. In a preferred embodiment, a first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.
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12.发明申请Methods of Synthesizing Heteromultimeric Polypeptides in Yeast Using a Haploid Mating Strategy 有权使用单倍体交配策略合成酵母中异源多聚体多肽的方法公开(公告)号：US20080003643A1
公开(公告)日：2008-01-03
申请号：US10577074
申请日：2004-10-22
IPC分类号： C12P21/00
CPC分类号： C07K16/2896 , C07K16/00 , C07K16/18 , C07K16/2809 , C07K2317/24 , C12N15/81 , C12N15/815
摘要： Methods are provided for the synthesis and secretion of recombinant hetero-multimeric proteins in mating competent yeast. A first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.
摘要翻译： 提供了用于在交配感受态酵母中合成和分泌重组异源多聚体蛋白的方法。 将第一表达载体转化为第一单倍体细胞; 并将第二表达载体转化成第二单倍体细胞。 鉴定转化的单倍体细胞，每个细胞各自合成不相同的多肽，然后遗传交叉或融合。 所得的二倍体菌株用于产生和分泌完全组装和生物功能的杂多聚体蛋白。
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13.发明申请Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy 有权使用单倍体交配策略在酵母中合成异源多聚体多肽的方法公开(公告)号：US20060270045A1
公开(公告)日：2006-11-30
申请号：US11429053
申请日：2006-05-08
申请人： James Cregg , John Latham , Mark Litton , Randall Schatzman , Ilya Tolstorukov
发明人： James Cregg , John Latham , Mark Litton , Randall Schatzman , Ilya Tolstorukov
CPC分类号： C07K16/2896 , C07K16/00 , C07K16/18 , C07K16/2809 , C07K2317/24 , C12N15/81 , C12N15/815
摘要： Methods are provided for the synthesis and secretion of recombinant proteins preferably large mammalian proteins or hetero-multimeric proteins at high levels and for prolonged time in polyploid, preferably diploid yeast. These methods use various mating competent yeast, including Pichia. In a preferred embodiment, a first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.
摘要翻译： 提供了用于在多倍体，优选二倍体酵母中以高水平合成和分泌重组蛋白，优选大量哺乳动物蛋白或杂多聚体蛋白并延长时间的方法。 这些方法使用各种交配的感受态酵母，包括毕赤酵母。 在优选的实施方案中，将第一表达载体转化为第一单倍体细胞; 并将第二表达载体转化成第二单倍体细胞。 鉴定转化的单倍体细胞，每个细胞各自合成不相同的多肽，然后遗传交叉或融合。 所得的二倍体菌株用于产生和分泌完全组装和生物功能的杂多聚体蛋白。
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公开(公告)号：US08337847B2
公开(公告)日：2012-12-25
申请号：US12624816
申请日：2009-11-24
IPC分类号： A61K38/20 , A61K39/395
CPC分类号： C07K16/248 , A61K39/3955 , A61K45/06 , A61K2039/505 , A61K2039/545 , C07K2317/34 , C07K2317/76 , C07K2317/92 , Y02A50/412
摘要： The invention provides methods of treating anemia in patients in need thereof, particularly in patients having anemia associated with chronic and/or inflammatory conditions such as cancers and arthritic conditions by administering antibodies or antibody fragments that bind IL-6. The treatment methods result in increased hemoglobin.
摘要翻译： 本发明提供了在有需要的患者中治疗贫血的方法，特别是通过施用结合IL-6的抗体或抗体片段，与慢性和/或炎性病症如癌症和关节炎病症相关的贫血患者。 治疗方法导致血红蛋白升高。
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15.发明授权公开(公告)号：US07927863B2
公开(公告)日：2011-04-19
申请号：US11429053
申请日：2006-05-08
CPC分类号： C07K16/2896 , C07K16/00 , C07K16/18 , C07K16/2809 , C07K2317/24 , C12N15/81 , C12N15/815
摘要： Methods are provided for the synthesis and secretion of recombinant proteins preferably large mammalian proteins or hetero-multimeric proteins at high levels and for prolonged time in polyploid, preferably diploid yeast. These methods use various mating competent yeast, including Pichia. In a preferred embodiment, a first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.
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公开(公告)号：US08992904B2
公开(公告)日：2015-03-31
申请号：US13511385
申请日：2010-11-24
IPC分类号： A61K39/395 , C07K16/24 , A61K38/20 , A61K31/454 , A61K39/00
摘要： The present invention is directed to therapeutic methods and compositions, especially subcutaneous and intravenous composition using antibodies and fragments thereof having binding specificity for IL-6 to prevent or treat cachexia, fever, weakness and/or fatigue in a patient in need thereof. In preferred embodiments, the anti-IL-6 antibodies will be humanized and/or will be aglycosylated. Also, in preferred embodiments these patients will comprise those exhibiting (or at risk of developing) an elevated serum C-reactive protein level. In another preferred embodiment, the patient's survivability or quality of life will preferably be improved.
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公开(公告)号：US09724410B2
公开(公告)日：2017-08-08
申请号：US13511385
申请日：2010-11-24
IPC分类号： A61K39/395 , C07K16/24 , A61K38/20 , A61K31/454 , A61K45/06 , C07K16/46 , A61K39/00
CPC分类号： A61K39/3955 , A61K31/454 , A61K38/2013 , A61K38/208 , A61K45/06 , A61K49/0004 , A61K49/0058 , A61K51/1033 , A61K2039/505 , A61K2039/545 , C07K16/248 , C07K16/462 , C07K2317/24 , C07K2317/33 , C07K2317/34 , C07K2317/41 , C07K2317/56 , C07K2317/565 , C07K2317/76 , C07K2317/90 , C07K2317/92 , C07K2317/94 , G01N33/6863 , Y02A50/412 , Y02A50/58
摘要： The present invention is directed to therapeutic methods and compositions, especially subcutaneous and intravenous composition using antibodies and fragments thereof having binding specificity for IL-6 to prevent or treat cachexia, fever, weakness and/or fatigue in a patient in need thereof. In preferred embodiments, the anti-IL-6 antibodies will be humanized and/or will be aglycosylated. Also, in preferred embodiments these patients will comprise those exhibiting (or at risk of developing) an elevated serum C-reactive protein level. In another preferred embodiment, the patient's survivability or quality of life will preferably be improved.
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公开(公告)号：US20050282754A1
公开(公告)日：2005-12-22
申请号：US11184459
申请日：2005-07-18
申请人： John Feder , Randall Schatzman , Pamela Bjorkman , Melanie Bennett , Jose Lebron
发明人： John Feder , Randall Schatzman , Pamela Bjorkman , Melanie Bennett , Jose Lebron
CPC分类号： C07K14/70539 , A61K38/00
摘要： The present invention relates to peptides and peptide analogues designed from HFE protein. In particular, it relates to peptides and peptide analogues designed from an alpha-1 region of HFE protein which lowers the binding affinity of transferrin receptor for transferrin. Such compounds mimic HFE protein function, and reduce iron uptake and/or accumulation by a cell.
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公开(公告)号：US20050090430A1
公开(公告)日：2005-04-28
申请号：US10956250
申请日：2004-10-01
申请人： John Feder , Randall Schatzman , Zenta Tsuchihashi
发明人： John Feder , Randall Schatzman , Zenta Tsuchihashi
摘要： Methods and compositions are provided for the diagnosis and treatment of iron misregulation diseases, including HFE polypeptides, agonists, and antagonists, and transferrin receptor agonists and antagonists.
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