公开(公告)号：US12040048B2

公开(公告)日：2024-07-16

申请号：US18170797

申请日：2023-02-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Fiona Hyland ,  Asha Kamat ,  Timothy Looney

IPC: G16B20/20 ,  G06F16/2457 ,  G06F16/248 ,  G06N3/126

CPC classification number: G16B20/20 ,  G06F16/24575 ,  G06F16/248 ,  G06N3/126

Abstract: The method includes compressing numbers of reads data for targeted genes of a gene expression assay performed on a test sample. The targeted genes are organized into categories. Each category represents a functional context associated with the targeted genes in that category. The numbers of reads corresponding to targeted genes each category is compressed to form a compressed value for the category. The compressed value is compared to a baseline value for the category to determine an enrichment or a loss of a signature corresponding to the functional context of the category. The method may include analyzing information from multiple assays performed on the test sample, assigning a score value to each assay result and predicting a response to immune-oncology treatment based on the assigned scores.

公开(公告)号：US20230340602A1

公开(公告)日：2023-10-26

申请号：US18318402

申请日：2023-05-16

Applicant: Life Technologies Corporation

Inventor： Geoffrey Lowman ,  Chenchen Yang ,  Timothy Looney

IPC: C12Q1/6883 ,  C12Q1/6869 ,  G16B50/30 ,  G16B40/10

CPC classification number: C12Q1/6883 ,  C12Q1/6869 ,  G16B50/30 ,  G16B40/10 ,  C12Q2600/112

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, methods provide for determining convergence of T cell receptor beta and T cell receptor gamma repertoires in samples prior to a treatment and predicting a subject's response to the treatment. In another aspect, methods provide predicting a subject's potential or predisposition to be protected from or vulnerable to an adverse event following a treatment.

公开(公告)号：US20230055712A1

公开(公告)日：2023-02-23

申请号：US17871235

申请日：2022-07-22

Applicant: Life Technologies Corporation

Inventor： Timothy Looney

IPC: C12Q1/6881

Abstract: The present disclosure provides methods for predicting clinical response to therapy of a subject with an autoimmune disease or disorder and/or methods for predicting prognosis of a subject with immunodeficiency (e.g., leukemia) based on characterizing the B cell immune repertoire of the subject. In one aspect, methods provide for determining frequency of class switch recombination and/or somatic hypermutation of B cell receptor repertoires in samples prior to a treatment and predicting a subject's prognosis and/or response to treatment based on the measured class switching and/or somatic hypermutation frequency. In another aspect, methods provide for determination of immune receptor class switching and/or somatic hypermutation frequency and treating a subject based on the resulting prediction of prognosis and/or response to treatment based on the measured class switching and/or somatic hypermutation frequency.

公开(公告)号：US11008609B2

公开(公告)日：2021-05-18

申请号：US16120045

申请日：2018-08-31

Applicant: Life Technologies Corporation

Inventor： Geoffrey Lowman ,  Timothy Looney ,  Lifeng Lin ,  Elizabeth Linch ,  Lauren Miller

IPC: C40B50/06 ,  C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6869 ,  C40B50/00

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire using genomic DNA from a biological sample. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Nucleic acid sequences of variable regions associated with the immune cell receptor are determined to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.