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    METHODS FOR DETECTION OF BOTULINUM NEUROTOXIN
    12.
    发明申请
    METHODS FOR DETECTION OF BOTULINUM NEUROTOXIN 有权
    检测嗜碱性粒细胞毒素的方法

    公开(公告)号：US20090176259A1

    公开(公告)日：2009-07-09

    申请号：US12134092

    申请日：2008-06-05

    申请人： Markus Kalkum ,  Karine Bagramyan

    发明人： Markus Kalkum ,  Karine Bagramyan

    IPC分类号： G01N33/573 ,  G01N33/569

    CPC分类号： G01N33/573 ,  C07K7/08 ,  C07K14/705 ,  G01N33/5308 ,  G01N33/542 ,  G01N33/569 ,  G01N2333/32 ,  G01N2333/33 ,  G01N2333/38

    摘要： Provided herein is a large immuno-sorbent surface area assay (ALISSA) for rapid and sensitive detection of toxin or enzyme activity. This assay is designed to capture a low number of toxin or enzyme molecules and to measure their intrinsic protease activity via conversion of a fluorigenic or luminescent substrate. The ALISSA is significantly faster and more sensitive than methods routinely utilized in the art. This assay is applicable for use for detection of a variety of toxins or enzymes having proteolytic activity, such as botulinum neurotoxin, bacillus anthracis lethal factor, human chitinases, and aspergillus fumigatus proteases. Also provided are methods for constructing and identifying novel luminescent or fluorescent substrates suitable for use with the ALISSA method.

    摘要翻译： 本文提供了用于快速和灵敏检测毒素或酶活性的大型免疫吸附剂表面积测定（ALISSA）。 该测定被设计为捕获少量的毒素或酶分子，并通过转化荧光或发光底物来测量它们的内在蛋白酶活性。 ALISSA比本领域常规使用的方法显着更快和更灵敏。 该测定适用于检测各种具有蛋白水解活性的毒素或酶，如肉毒杆菌神经毒素，炭疽杆菌致死因子，人几丁质酶和烟曲霉蛋白酶。 还提供了用于构建和鉴定适用于ALISSA方法的新型发光或荧光底物的方法。

    METHODS FOR DETECTION OF BOTULINUM NEUROTOXIN
    14.
    发明申请
    METHODS FOR DETECTION OF BOTULINUM NEUROTOXIN 审中-公开
    检测嗜碱性粒细胞毒素的方法

    公开(公告)号：US20120322081A1

    公开(公告)日：2012-12-20

    申请号：US13306769

    申请日：2011-11-29

    申请人： Markus Kalkum ,  Karine Bagramyan

    发明人： Markus Kalkum ,  Karine Bagramyan

    IPC分类号： G01N21/76

    CPC分类号： G01N33/573 ,  C07K7/08 ,  C07K14/705 ,  G01N33/5308 ,  G01N33/542 ,  G01N33/569 ,  G01N2333/32 ,  G01N2333/33 ,  G01N2333/38

    摘要： Provided herein is a large immuno-sorbent surface area assay (ALISSA) for rapid and sensitive detection of toxin or enzyme activity. This assay is designed to capture a low number of toxin or enzyme molecules and to measure their intrinsic protease activity via conversion of a fluorigenic or luminescent substrate. The ALISSA is significantly faster and more sensitive than methods routinely utilized in the art. This assay is applicable for use for detection of a variety of toxins or enzymes having proteolytic activity, such as botulinum neurotoxin, bacillus anthracis lethal factor, human chitinases, and aspergillus fumigatus proteases. Also provided are methods for constructing and identifying novel luminescent or fluorescent substrates suitable for use with the ALISSA method.

    摘要翻译： 本文提供了用于快速和灵敏检测毒素或酶活性的大型免疫吸附剂表面积测定（ALISSA）。 该测定被设计为捕获少量的毒素或酶分子，并通过转化荧光或发光底物来测量它们的内在蛋白酶活性。 ALISSA比本领域常规使用的方法显着更快和更灵敏。 该测定适用于检测各种具有蛋白水解活性的毒素或酶，如肉毒神经毒素，炭疽杆菌致死因子，人几丁质酶和烟曲霉蛋白酶。 还提供了用于构建和鉴定适用于ALISSA方法的新型发光或荧光底物的方法。

    Method and system for mass spectroscopy
    15.
    发明申请
    Method and system for mass spectroscopy 有权
    质谱方法和系统

    公开(公告)号：US20050092912A1

    公开(公告)日：2005-05-05

    申请号：US10952529

    申请日：2004-09-28

    申请人： Andrew Krutchinsky ,  Herbert Cohen ,  Markus Kalkum ,  Vadim Sherman ,  Brian Chait

    发明人： Andrew Krutchinsky ,  Herbert Cohen ,  Markus Kalkum ,  Vadim Sherman ,  Brian Chait

    IPC分类号： H01J49/16 ,  H01J49/42 ,  H01J49/00 ,  B01D59/44 ,  H01J49/40

    CPC分类号： H01J49/063 ,  H01J49/0481 ,  H01J49/161 ,  H01J49/164

    摘要： A system for determining the ratio of mass to charge of an ion including a pulsed ionizer, a high pressure co-linear ion guide/accelerator, and a mass analyzer. The pulsed ionizer generates intact analyte ions from a sample of matter to be analyzed. The high pressure co-linear ion guide/accelerator is interfaced with the ion source for receipt of the intact ions of the sample. The ion guide/accelerator simultaneously dampens and linearly accelerates the intact ions in the substantial absence of fragmentation of the ions to provide a substantially continuous beam of the intact ions for mass analysis. The mass analyzer is connected to the ion guide/accelerator for receipt of the beam of ions and determines the mass to charge ratio of the intact ions.

    摘要翻译： 用于确定包括脉冲离子发生器，高压共线性离子导向器/加速器和质量分析器的离子的质量与电荷的比率的系统。 脉冲电离器从待分析的物质样品中产生完整的分析物离子。 高压共线离子导向器/加速器与离子源接口以接收样品的完整离子。 离子导向器/加速器在基本上不存在离子碎裂的同时抑制和线性加速完整离子，以提供基本上连续的完整离子束用于质量分析。 质量分析器连接到离子导向器/加速器以接收离子束，并确定完整离子的质荷比。