公开(公告)号：US20050042714A1

公开(公告)日：2005-02-24

申请号：US10487023

申请日：2002-08-17

申请人： Michael Pawlita ,  Cornelia Oetke ,  Oliver Keppler ,  Reinhard Brossmer ,  Stephan Hinderlich ,  Werner Reutter

发明人： Michael Pawlita ,  Cornelia Oetke ,  Oliver Keppler ,  Reinhard Brossmer ,  Stephan Hinderlich ,  Werner Reutter

IPC分类号： A61K38/00 ,  C07K14/47 ,  A61K38/16 ,  C07K9/00 ,  C12P21/06

CPC分类号： C07K14/4702 ,  A61K38/00

摘要： The present invention relates to glycoconjugates containing a sialic acid derivate of general formula (I) and wherein the sialic acid derivate of general formula (I) is conjugated to a mono-, di- or oligosaccharide with up to 40 glycosidically linked, optionally branched sugar residues representing furanose and/or pyranose rings, which are linked N- or O-glycosidically to the polypeptide. The sialic derivatives of general formula (I) are useful for producing pharmaceutical compositions for immunosuppression, cell protection, stimulation of hematopoesis regulation of hormonal secretion and hormonal activation.

摘要翻译： 本发明涉及含有通式（I）的唾液酸衍生物的糖缀合物，其中通式（I）的唾液酸衍生物与单糖，二糖或寡糖与多达40个糖苷连接的任选支链糖 表示呋喃糖和/或吡喃糖环的残基，其与多肽连接在N-或O-糖苷上。 通式（I）的唾液酸衍生物可用于制备用于免疫抑制，细胞保护，刺激造血调节激素分泌和激素激活的药物组合物。

公开(公告)号：US20130149696A1

公开(公告)日：2013-06-13

申请号：US13700672

申请日：2011-05-31

申请人： Michael Pawlita ,  Ivonne Rubio ,  Martin Müller ,  Peter Sehr

发明人： Michael Pawlita ,  Ivonne Rubio ,  Martin Müller ,  Peter Sehr

IPC分类号： G01N33/68

CPC分类号： G01N33/6854 ,  C12Q1/66 ,  C12Q1/6813 ,  C12Q1/70

摘要： The present invention relates to a method for the determination of the presence of PV-neutralizing antibodies in a sample, comprising a) contacting said sample with infectious PV particles comprising a reporter gene, wherein the gene product of said reporter gene is secreted into the growth medium, b) contacting the PV particles from a) with host cells, and c) determining PV-neutralizing antibodies based on the amount of gene product from said reporter gene, wherein, preferably, a lower amount of said gene product as compared to a reference amount is indicative of the presence of PV-neutralizing antibodies. It further relates to a host cell strongly adhering to multi-cluster plates for use in a method for diagnosing anti-PV immunity comprising the method of the present invention.

摘要翻译： 本发明涉及一种用于测定样品中PV中和抗体的存在的方法，包括a）使所述样品与包含报道基因的感染性PV颗粒接触，其中所述报告基因的基因产物分泌到生长中 培养基，b）使来自a）的PV颗粒与宿主细胞接触，以及c）基于来自所述报道基因的基因产物的量测定PV中和抗体，其中优选地，较低量的所述基因产物与 参考量表明PV中和抗体的存在。 本发明还涉及一种用于诊断抗PV免疫的方法的多簇板的宿主细胞，其包括本发明的方法。

公开(公告)号：US06214541B1

公开(公告)日：2001-04-10

申请号：US08981870

申请日：1998-03-09

申请人： Hanswalter Zentgraf ,  Manfred Frey ,  Iris Velhagen ,  Regina Martens ,  Wolfgang Meschede ,  Michael Pawlita ,  Joris Braspenning ,  Masimo Tommasino

发明人： Hanswalter Zentgraf ,  Manfred Frey ,  Iris Velhagen ,  Regina Martens ,  Wolfgang Meschede ,  Michael Pawlita ,  Joris Braspenning ,  Masimo Tommasino

IPC分类号： G01N3368

CPC分类号： C07K14/005 ,  C07K2319/00 ,  C12N2710/20022 ,  G01N33/54306 ,  G01N33/56983 ,  G01N33/6854 ,  G01N2333/025 ,  Y10S436/815 ,  Y10S530/826

摘要： The present invention relates to a method of detecting specific antibodies directed against HPV proteins in body fluids, comprising the following steps: (I) incubating a native carrier material-bound HPV protein with body fluids, and (II) reacting specific antibodies (a) bound to the HPV protein with labeled antibodies (b) directed against antibodies (a) or with unlabeled antibodies (b) and the latter with labeled antibodies (c) directed against antibodies (b). Furthermore, this invention concerns a kit usable for this purpose.

摘要翻译： 本发明涉及一种检测针对体液中HPV蛋白的特异性抗体的方法，包括以下步骤：（I）将天然载体材料结合的HPV蛋白质与体液孵育，和（II）使特异性抗体（a） （b）针对抗体（a）或未标记抗体（b）的HPV蛋白与后者与针对抗体的标记抗体（c）结合（b）。此外，本发明涉及可用于该目的的试剂盒。