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    HIGH-THROUGHPUT METHOD FOR DETERMINING THE PRESENCE OF PAPILLOMAVIRUS-NEUTRALIZING ANTIBODIES IN A SAMPLE
    1.
    发明申请
    HIGH-THROUGHPUT METHOD FOR DETERMINING THE PRESENCE OF PAPILLOMAVIRUS-NEUTRALIZING ANTIBODIES IN A SAMPLE 审中-公开
    用于确定样品中斑马鱼中和抗体存在的高通量方法

    公开(公告)号：US20130149696A1

    公开(公告)日：2013-06-13

    申请号：US13700672

    申请日：2011-05-31

    申请人： Michael Pawlita ,  Ivonne Rubio ,  Martin Müller ,  Peter Sehr

    发明人： Michael Pawlita ,  Ivonne Rubio ,  Martin Müller ,  Peter Sehr

    IPC分类号： G01N33/68

    CPC分类号： G01N33/6854 ,  C12Q1/66 ,  C12Q1/6813 ,  C12Q1/70

    摘要： The present invention relates to a method for the determination of the presence of PV-neutralizing antibodies in a sample, comprising a) contacting said sample with infectious PV particles comprising a reporter gene, wherein the gene product of said reporter gene is secreted into the growth medium, b) contacting the PV particles from a) with host cells, and c) determining PV-neutralizing antibodies based on the amount of gene product from said reporter gene, wherein, preferably, a lower amount of said gene product as compared to a reference amount is indicative of the presence of PV-neutralizing antibodies. It further relates to a host cell strongly adhering to multi-cluster plates for use in a method for diagnosing anti-PV immunity comprising the method of the present invention.

    摘要翻译： 本发明涉及一种用于测定样品中PV中和抗体的存在的方法，包括a）使所述样品与包含报道基因的感染性PV颗粒接触，其中所述报告基因的基因产物分泌到生长中 培养基，b）使来自a）的PV颗粒与宿主细胞接触，以及c）基于来自所述报道基因的基因产物的量测定PV中和抗体，其中优选地，较低量的所述基因产物与 参考量表明PV中和抗体的存在。 本发明还涉及一种用于诊断抗PV免疫的方法的多簇板的宿主细胞，其包括本发明的方法。

    PROBES FOR GENOTYPING LOW-RISK-HPV
    4.
    发明申请
    PROBES FOR GENOTYPING LOW-RISK-HPV 审中-公开
    探索低风险HPV的基因研究

    公开(公告)号：US20130116136A1

    公开(公告)日：2013-05-09

    申请号：US13515757

    申请日：2010-12-14

    申请人： Markus Schmitt ,  Michael Pawlita

    发明人： Markus Schmitt ,  Michael Pawlita

    IPC分类号： C12Q1/70

    CPC分类号： C12Q1/708

    摘要： The current invention is concerned with a composition comprising at least one probe oligonucleotide each for the nucleotide sequences of the invention, said probe oligonucleotides specifically hybridizing to the sense strand or the antisense strand of said nucleotide sequences. Moreover, the present invention relates to a method for the identification of low-risk HPV types in a sample comprising the steps of a) contacting a sample with an amplification composition allowing amplification of at least one region of the HPV genome specifically hybridizing to at least one of the probe oligonucleotides of the current invention under conditions which allow for the amplification of polynucleotides and b) identifying low-risk HPV genotypes in said sample based on the amplified polynucleotides obtained in step a) by hybridizing the amplified polynucleotides with at least one labelled probe oligonucleotide of the current invention while said amplified polynucleotides are present in the same reaction container. The invention also relates to the use of a composition of the current invention for identifying low-risk HPV hi a sample and to a kit comprising the composition of the invention and/or adopted for carrying out the method of the invention and an instruction manual.

    摘要翻译： 本发明涉及包含至少一种针对本发明的核苷酸序列的探针寡核苷酸的组合物，所述探针寡核苷酸与所述核苷酸序列的有义链或反义链特异性杂交。 此外，本发明涉及一种用于鉴定样品中低风险HPV类型的方法，包括以下步骤：a）使样品与扩增组合物接触，所述扩增组合物允许扩增HPV基因组的至少一个区域，特异性杂交至少 在允许扩增多核苷酸的条件下，本发明的探针寡核苷酸之一，以及b）基于步骤a）中获得的扩增多核苷酸鉴定所述样品中的低风险HPV基因型，通过将扩增的多核苷酸与至少一个标记的 而所述扩增的多核苷酸存在于同一反应容器中的本发明的探针寡核苷酸。 本发明还涉及本发明的组合物在样品中鉴定低风险HPV的用途，以及包含本发明组合物和/或用于实施本发明方法和使用说明书的试剂盒的用途。

    Diagnostic Transcript and Splice Patterns of HPV16 in Different Cervical Lesions
    5.
    发明申请
    Diagnostic Transcript and Splice Patterns of HPV16 in Different Cervical Lesions 有权
    HPV16在不同宫颈病变中的诊断睾丸和融合模式

    公开(公告)号：US20120040334A1

    公开(公告)日：2012-02-16

    申请号：US13128201

    申请日：2009-11-09

    申请人： Markus Schmitt ,  Lutz Gissmann ,  Michael Pawlita

    发明人： Markus Schmitt ,  Lutz Gissmann ,  Michael Pawlita

    IPC分类号： C12Q1/70 ,  C12M1/34

    CPC分类号： C12Q1/708 ,  C12Q2545/113

    摘要： The present invention relates to a method for differentiating in a subject with HPV16 between (i) a severe form of HPV16 infection and (ii) a mild form of HPV16 infection based on determining the amount of a first gene product and a second gene product in a sample of a subject and calculating a ratio of the amount of said first gene product and the amount of said second gene product. Further envisaged by the present invention is a composition comprising an oligonucleotide mixture. Also envisaged by the present invention are a kit and a device adapted to carry out the method of the present invention.

    摘要翻译： 本发明涉及一种用于在（i）严重形式的HPV16感染和（ii）轻度形式的HPV16感染之间的HPV16差异化的方法，其基于确定第一基因产物和第二基因产物的量 计算受试者的样品并计算所述第一基因产物的量与所述第二基因产物的量的比率。 本发明进一步设想的是包含寡核苷酸混合物的组合物。 本发明还设想了适用于实施本发明方法的试剂盒和装置。

    Composition comprising an oligonucleotide mixture for the detection of contaminations in cell cultures
    7.
    发明授权
    Composition comprising an oligonucleotide mixture for the detection of contaminations in cell cultures 有权
    组合物，其包含用于检测细胞培养物中污染物的寡核苷酸混合物

    公开(公告)号：US09034604B2

    公开(公告)日：2015-05-19

    申请号：US12920095

    申请日：2009-03-05

    申请人： Markus Schmitt ,  Michael Pawlita

    发明人： Markus Schmitt ,  Michael Pawlita

    IPC分类号： C12Q1/68 ,  C12Q1/70

    CPC分类号： C12Q1/689 ,  C12Q1/686 ,  C12Q1/6881 ,  C12Q1/701 ,  C12Q1/702 ,  C12Q1/705 ,  C12Q2600/16

    摘要： The present invention relates to a method for determining contaminations in a cell culture sample comprising the steps of: a) contacting a sample of a cell culture suspected to comprise contaminations with a composition comprising oligonucleotides under conditions which allow for amplification of polynucleotides, wherein said oligonucleotides comprise oligonucleotides of at least three different groups of oligonucleotides, and b) determining the contaminations based on the amplified polynucleotides obtained by using the oligonucleotide groups of step (a). Moreover, the invention relates to a composition comprising an oligonucleotide mixture. Further encompassed by the present invention is a composition comprising a probe oligonucleotide mixture. Finally, the present invention also relates to kits comprising said oligonucleotide mixtures.

    摘要翻译： 本发明涉及一种用于确定细胞培养物样品中污染物的方法，包括以下步骤：a）在允许扩增多核苷酸的条件下，将怀疑含有污染物的细胞培养物样品与包含寡核苷酸的组合物接触，其中所述寡核苷酸 包含至少三种不同组寡核苷酸的寡核苷酸，和b）基于通过使用步骤（a）的寡核苷酸组获得的扩增多核苷酸来测定污染物。 此外，本发明涉及包含寡核苷酸混合物的组合物。 本发明进一步包括的是包含探针寡核苷酸混合物的组合物。 最后，本发明还涉及包含所述寡核苷酸混合物的试剂盒。

    MEANS AND METHODS FOR PREDICTING THE RISK OF MORTALITY OF PATIENTS WITH HPV POSITIVE OROPHARYNGEAL SQUAMOUS CELL CANCER
    10.
    发明申请
    MEANS AND METHODS FOR PREDICTING THE RISK OF MORTALITY OF PATIENTS WITH HPV POSITIVE OROPHARYNGEAL SQUAMOUS CELL CANCER 审中-公开
    用于预测HPV阳性OROPHARYNGEAL SQUAMOUS细胞癌患者的死亡风险的手段和方法

    公开(公告)号：US20130029319A1

    公开(公告)日：2013-01-31

    申请号：US13556588

    申请日：2012-07-24

    申请人： Markus SCHMITT ,  Michael Pawlita ,  Dana Holzinger ,  Franz Bosch

    发明人： Markus SCHMITT ,  Michael Pawlita ,  Dana Holzinger ,  Franz Bosch

    IPC分类号： C12Q1/70

    CPC分类号： C12Q1/6886 ,  C12Q1/708 ,  C12Q2600/118 ,  C12Q2600/158

    摘要： The present invention relates to the field of diagnostic measures. In particular, the present invention relates to a method for predicting the risk of mortality in a subject suffering from low viral load HPV (human papillomavirus) positive oropharyngeal squamous cell cancer. The method is based on the determination of the amount of an E6* gene product and the amount an E1̂E4 gene product of HPV in a sample from said subject. Moreover, the method is based on determining the presence of absence of an E1C gene product of HPV. The present invention further relates to a method for predicting the risk of mortality in a subject suffering from HPV (human papillomavirus) positive oropharyngeal squamous cell cancer based on the determination of copy number of HPV per cancer cell.

    摘要翻译： 本发明涉及诊断措施领域。 特别地，本发明涉及用于预测患有低病毒载量HPV（人乳头瘤病毒）阳性口咽鳞状细胞癌的受试者的死亡风险的方法。 该方法基于来自所述受试者的样品中E6 *基因产物的量的确定和HPV的E1E4基因产物的量的量。 此外，该方法基于确定HPV的E1C基因产物的存在。 本发明还涉及基于每个癌细胞的HPV拷贝数的确定来预测患有HPV（人乳头瘤病毒）阳性口咽鳞状细胞癌的受试者的死亡风险的方法。