公开(公告)号：US4588492A

公开(公告)日：1986-05-13

申请号：US609404

申请日：1984-05-07

申请人： Milan Bier

发明人： Milan Bier

IPC分类号： B01D57/02 ,  G01N27/447 ,  G01N27/28

CPC分类号： G01N27/44795 ,  B01D57/02

摘要： This disclosure is directed to an isoelectric focusing apparatus, wherein stabilization of the fluid containing the isolated proteins is achieved by carrying out the separation in a rotating cylinder with the separation cavity of the cylinder being segmented by means of filter elements. The filter elements are constituted of a material offering some degree of resistance to fluid convection, but allowing relatively free and unhindered passage of current and transport of proteins. The combined effect of segmentation and rotation has been found to be superior to either segmentation or rotation alone in maintaining the stability of the migrated fractions.

摘要翻译： 本公开涉及一种等电聚焦装置，其中包含分离的蛋白质的流体的稳定性通过在旋转圆筒中进行分离来实现，其中圆筒的分离腔通过过滤元件分段。 过滤元件由对流体对流提供一定程度的抵抗力的材料构成，但允许蛋白质相对自由和不受限制地通过电流和转运。 已经发现分割和旋转的组合效应在维持迁移的级分的稳定性方面优于单独的分割或旋转。

公开(公告)号：US4406886A

公开(公告)日：1983-09-27

申请号：US458128

申请日：1983-01-14

申请人： Milan Bier ,  Peter R. Foster

发明人： Milan Bier ,  Peter R. Foster

IPC分类号： A61K38/00 ,  C07K14/755 ,  A61K35/14

CPC分类号： C07K14/755 ,  A61K38/00

摘要： The present invention relates to the reduction of the fibrinogen concentration in cryoprecipitate fraction formed during the fractionation of blood. This zinc-mediated decrease of fibrinogen increases the ratio of Factor VIII to total protein content.

摘要翻译： 本发明涉及在分离血液期间形成的冷沉淀物级分中纤维蛋白原浓度的降低。 这种锌介导的纤维蛋白原减少增加了因子VIII与总蛋白质含量的比例。

公开(公告)号：US4362612A

公开(公告)日：1982-12-07

申请号：US99499

申请日：1979-12-03

申请人： Milan Bier

发明人： Milan Bier

IPC分类号： B01D57/02 ,  G01N27/447 ,  B01D13/02 ,  G01N27/26

CPC分类号： G01N27/44795 ,  B01D57/02 ,  G01N27/44773

摘要： The disclosure is directed to an apparatus for isoelectric focusing. A fluid is employed which contains buffering compounds capable of establishing a pH gradient in an electric field. A plurality of ion non-selective permeable membranes are used to streamline the flow of fluid while allowing interchange of fluid constituents therebetween. Electrodes establish an electrical potential transverse the flow of fluid to establish a gradient of pH steps as between successive channels defined by the membranes.

摘要翻译： 本公开涉及用于等电聚焦的装置。 使用含有能够在电场中建立pH梯度的缓冲化合物的流体。 使用多个离子非选择性渗透膜来简化流体流动，同时允许流体组分之间的互换。 电极建立横向于流体流动的电势，以建立由膜限定的连续通道之间的pH步长梯度。

公开(公告)号：US4204929A

公开(公告)日：1980-05-27

申请号：US897261

申请日：1978-04-18

申请人： Milan Bier

发明人： Milan Bier

IPC分类号： B01D57/02 ,  B01D61/42 ,  G01N27/447 ,  B01D13/02 ,  G01N27/26

CPC分类号： G01N27/44795 ,  B01D57/02

摘要： The disclosure is directed to a method and apparatus for isoelectric focusing of fluids. In accordance with the disclosed method, the flow of fluids to be processed is established in a first direction. This flow of fluids is streamlined by providing a plurality of permeable microporous membranes which define generally parallel channels oriented in the first direction. An electrical potential is applied across the streamlined channels of flowing fluid, and isoelectric focusing is achieved on the fluids during the flow thereof since the membranes allow interchange of fluid constituents therebetween while providing the desired streamlining. An approximation of "plug" type flow is achieved within the streamlined channels; i.e., flow having an approximately uniform cross-sectional characteristic. In the preferred embodiment of the method of the invention, a recirculation path is established for each of the streamlined channels, such that the fluid flowing out of each channel is recirculated back to the beginning of the channel. Preferably, this is achieved by pumping the fluids in each of the recirculation paths, and also providing cooling for the fluids during the recirculation thereof. In this manner, a number of "passes" are effected to obtain the desired degree of isoelectric focusing. The cooling, which is performed during the recirculation, serves to minimize problems with dissipation of Joule heat during the isoelectric focusing of the fluids in the streamlined channels. Also, in the preferred embodiment of the invention, the first direction is downward such that the streamlined fluids flow under the influence of gravity to permit gravity equilibrium of fluid levels in the channels across the streamlining membranes.

摘要翻译： 本公开涉及一种用于流体等离子体聚焦的方法和装置。 根据所公开的方法，在第一方向建立待处理流体的流动。 这种流体流动通过提供多个可渗透的微孔膜来流线，该多孔膜限定了沿第一方向定向的大致平行的通道。 在流动流体的流线型通道之间施加电势，并且在流体流动期间在流体上实现等电聚焦，因为膜允许在其间交换流体成分，同时提供所需的精简。 在流线型通道内实现了“插头”型流量的近似; 即具有大致均匀横截面特性的流动。 在本发明方法的优选实施例中，为每个流线型通道建立再循环路径，使得流出每个通道的流体再循环回通道的开头。 优选地，这通过在每个再循环路径中泵送流体来实现，并且还在其再循环期间为流体提供冷却。 以这种方式，进行许多“通过”以获得期望的等电聚焦程度。 在再循环期间进行的冷却用于在流线型通道中的流体的等电聚焦期间最小化焦耳热耗散的问题。 此外，在本发明的优选实施例中，第一方向是向下的，使得流线型流体在重力的作用下流动，以允许跨越流化膜的通道中的液位的重力平衡。