公开(公告)号：US5241048A

公开(公告)日：1993-08-31

申请号：US609946

申请日：1990-11-06

申请人： Leon E. Barstow ,  Glen D. Ward ,  Milan Bier

发明人： Leon E. Barstow ,  Glen D. Ward ,  Milan Bier

IPC分类号： C07K1/02 ,  C07K1/04

CPC分类号： C07K1/02 ,  C07K1/04 ,  C07K1/045 ,  Y02P20/544

摘要： Chemical reactions are readily carried out using supercritical carbon dioxide as the reaction medium. Supercritical carbon dioxide is of special value as a reaction medium in reactions for synthesizing polypeptides, for sequencing polypeptides, or for amino acid analysis.

摘要翻译： 使用超临界二氧化碳作为反应介质容易地进行化学反应。 超临界二氧化碳作为反应介质在合成多肽，多肽测序或氨基酸分析的反应中是特别有价值的。

公开(公告)号：US06793791B2

公开(公告)日：2004-09-21

申请号：US10374943

申请日：2003-02-26

申请人： Milan Bier

发明人： Milan Bier

IPC分类号： G01N2726

CPC分类号： G01N27/44795

摘要： An elastomeric cell is used as the disposable part of an apparatus for isoelectric focusing in free solution (without gels) in the 0.5 to 5 ml volume range. An inlet port is used for priming the cell and end-connectors for coupling with electrodes. A grid of parallel rods compresses the cell against a cold plate, thereby causing swelling of the skin of the cell between pairs of rods and forming contiguous fluid bubble-compartments for IEF separation. Before collection of separated fractions, the gap between the rods and the plate is further reduced so as to create distinct fluid compartments which now contain discrete products of separation. The separated fractions are collected by syringe-like devices by puncturing the elastomer skin. The plate, rods and deformable cell are capable of rotation or gentle rocking motion around the cell's main axis to avoid gravitational convection during the focusing process.

摘要翻译： 使用弹性体电池作为在0.5至5ml体积范围内的游离溶液（不含凝胶）中的等电聚焦装置的一次性部分。 入口用于引发电池和端接头，用于与电极耦合。 平行杆的格栅将细胞压在冷板上，从而导致细胞在成对的杆之间膨胀，并形成连续的液体气泡隔室以进行IEF分离。 在收集分离的馏分之前，进一步减少棒和板之间的间隙，以便产生不同的流体隔室，其现在包含离散的分离产物。 通过注射弹性体皮肤通过注射器状装置收集分离的级分。 板，杆和可变形单元能够围绕单元的主轴旋转或缓和摇摆运动，以避免聚焦过程中的重力对流。

公开(公告)号：US6096310A

公开(公告)日：2000-08-01

申请号：US839618

申请日：1997-04-15

申请人： Milan Bier

发明人： Milan Bier

IPC分类号： C07K16/06 ,  A61K39/395 ,  A61K39/40 ,  A61K39/42 ,  A61K39/44

CPC分类号： C07K16/06 ,  A61K2039/505

摘要： The object of the present invention is the oral administration of animal immunoglobulins for the treatment of gastrointestinal disorders caused by bacterial and/or yeast overgrowth. Immunoglobulins derived from the blood, plasma or serum of animals, such as cow, goats, sheep and pigs, contain a broad spectrum of antibodies to bacteria and yeast that afflict the gastrointestinal tract of human patients.

摘要翻译： 本发明的目的是口服给予动物免疫球蛋白用于治疗由细菌和/或酵母过度生长引起的胃肠道疾病。 衍生自动物（如牛，山羊，绵羊和猪）的血液，血浆或血清的免疫球蛋白含有广泛的细菌和酵母抗体，可以折磨人类患者的胃肠道。

公开(公告)号：US4486282A

公开(公告)日：1984-12-04

申请号：US468251

申请日：1983-02-22

申请人： Milan Bier

发明人： Milan Bier

IPC分类号： A23C9/144 ,  A23J1/06 ,  A23J1/20 ,  A61M1/34 ,  C07K1/30 ,  C07K1/36 ,  C07K14/76 ,  C07K16/06 ,  B01D13/02 ,  B01D13/00

CPC分类号： B01D61/44 ,  A23C9/144 ,  A23J1/06 ,  A23J1/205 ,  A61M1/3472 ,  A61M1/3482 ,  A61M1/3486 ,  B01D61/46 ,  C07K1/30 ,  C07K14/76 ,  C07K16/065 ,  A23V2002/00 ,  B01D2311/04 ,  B01D2311/2642

摘要： Total or fractional precipitation of proteins from salt-containing proteinaceous solution or suspension mixtures is induced or enhanced by subjecting the proteinaceous mixture to a desalting treatment, for example, by electrodialysis. In one aspect of the invention, the desalting treatment is used in conjunction with the addition of heavy metal ions to the proteinaceous mixture to provide a synergistic effect on the precipitation of the protein fraction. In another aspect of the invention, the desalting treatment, either with or without the heavy metal ion addition, is employed as an integral part of a selective plasmapheresis procedure for the removal or recovery of one or more selected plasma proteins from blood plasma.

摘要翻译： 通过例如通过电渗析对蛋白质混合物进行脱盐处理，诱导或增强来自含盐蛋白质溶液或悬浮混合物的蛋白质的总分或分数沉淀。 在本发明的一个方面，脱盐处理与向蛋白质混合物中加入重金属离子一起使用以对蛋白质级分的沉淀提供协同效应。 在本发明的另一方面，使用或不加入重金属离子添加剂的脱盐处理作为用于从血浆中去除或回收一种或多种选择的血浆蛋白质的选择性血浆置换方法的组成部分。

公开(公告)号：US5447612A

公开(公告)日：1995-09-05

申请号：US363640

申请日：1994-12-22

申请人： Milan Bier ,  Terry D. Long ,  Rodolfo B. Marguez ,  James A. Ostrem

发明人： Milan Bier ,  Terry D. Long ,  Rodolfo B. Marguez ,  James A. Ostrem

IPC分类号： G01N27/447 ,  C25B7/00

CPC分类号： G01N27/44795 ,  G01N27/44747

摘要： A novel pH buffering system which is functionally stable under electrophoretic conditions employs buffering components in complementary buffer pairs. The buffering components are selected from among simple chemically defined ampholytes, weak acids and weak bases, and are paired together on the basis of their dissociation characteristics to define narrow overlapping buffering zones in which the buffering components exhibit low net electrophoretic mobility combined with desired pH buffering capacity. The narrow buffering zones cover from 0.4 to 1.25 pH units within a broad range of pH values of from about pH 3 to about pH 10. Specific buffering pH values within the narrow buffering zones are obtainable by varying the molar ratios of the two buffering components of the selected buffer pair. The buffering system may be used to form functionally stable precast narrow pH zone gradients in free solution, and has particular utility in isoelectric focusing and other electrophoretic processes.

摘要翻译： 在电泳条件下功能稳定的新型pH缓冲系统使用互补缓冲液对中的缓冲组分。 缓冲组分选自简单的化学定义的两性电解质，弱酸和弱碱，并且基于它们的解离特性配对在一起，以限定窄的重叠缓冲区，其中缓冲组分表现出低的电泳迁移率与期望的pH缓冲相结合 容量。 窄的缓冲区域在pH范围为约pH 3至约pH 10的范围内，覆盖0.4至1.25个pH单位。窄缓冲区域内的特定缓冲pH值可通过改变两个缓冲组分的摩尔比来获得 所选择的缓冲区对。 缓冲系统可用于在游离溶液中形成功能稳定的预制窄pH区梯度，并且在等电聚焦和其他电泳过程中具有特殊的用途。

公开(公告)号：US5001224A

公开(公告)日：1991-03-19

申请号：US167201

申请日：1988-03-11

申请人： Leon E. Barstow ,  Glen D. Ward ,  Milan Bier

发明人： Leon E. Barstow ,  Glen D. Ward ,  Milan Bier

IPC分类号： C07D233/64 ,  C07B41/12 ,  C07B43/06 ,  C07C69/14 ,  C07C237/22 ,  C07K1/02 ,  C07K1/04

CPC分类号： C07K1/045 ,  C07K1/02 ,  C07K1/04 ,  Y02P20/544

摘要： Chemical reactions are readily carried out using supercritical carbon dioxide as the reaction medium. Supercritical carbon dioxide is of special value as a reaction medium in reactions for synthesizing polypeptides, for sequencing polypeptides, or for amino acid analysis.

摘要翻译： 使用超临界二氧化碳作为反应介质容易地进行化学反应。 超临界二氧化碳作为合成多肽的反应，多肽测序或氨基酸分析的反应介质是特别有价值的。

公开(公告)号：US4040940A

公开(公告)日：1977-08-09

申请号：US753103

申请日：1976-12-21

申请人： Milan Bier

发明人： Milan Bier

IPC分类号： G01N27/447 ,  G01N27/26 ,  G01N27/28

CPC分类号： G01N27/44717 ,  G01N27/44704

摘要： Electrophoretic fractional elution apparatus has a column with a rotating seal joint at which a thin jet of eluting buffer is directed across the lumen of the electrophoretic column in a direction perpendicular to that of electrophoretic migration. Either the content of the column is rotated with respect to the stationary jet, or the jet is rotated with respect to the column. The system may employ electrophoresis either in free solution or in packed columns.

摘要翻译： 电泳分级洗脱装置具有带有旋转密封接头的柱，在该柱上，洗脱缓冲液的薄射流沿垂直于电泳迁移的方向穿过电泳柱的内腔。 柱的内容物相对于静止射流旋转，或者射流相对于柱旋转。 该系统可以在游离溶液或填充柱中使用电泳。

公开(公告)号：US5980839A

公开(公告)日：1999-11-09

申请号：US22047

申请日：1998-02-11

申请人： Milan Bier ,  German Eduardo Fuentes ,  Rodolfo Bacheleir Marquez ,  Anthony Ralph Ford

发明人： Milan Bier ,  German Eduardo Fuentes ,  Rodolfo Bacheleir Marquez ,  Anthony Ralph Ford

IPC分类号： B01J19/00 ,  C07B61/00 ,  C07K1/04 ,  C40B40/10 ,  C40B60/14 ,  G01N35/00 ,  B01J8/08

CPC分类号： C07K1/045 ,  B01J19/0046 ,  C07K1/047 ,  B01J2219/00286 ,  B01J2219/00306 ,  B01J2219/00416 ,  B01J2219/00423 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00481 ,  B01J2219/00488 ,  B01J2219/00495 ,  B01J2219/005 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00689 ,  B01J2219/00725 ,  C40B40/10 ,  C40B60/14 ,  G01N2035/00564 ,  G01N2035/00574

摘要： The objective of combinatorial chemical synthesis is the preparation of a very large number of different molecular entities by a randomized process of sequential chemical syntheses. The present invention provides a combinatorial vessel comprising a reaction region and a mixing region, adjacent to each other, which can be used to practice combinatorial chemical synthesis. The reaction region comprises a plurality of reaction cavities, in which a plurality of different chemical reactions may be performed simultaneously on resin beads. The resin beads are then transferred to the mixing region and mixed into a single group. Then, the resin beads are transferred into the reaction cavities. The process of performing reactions, transferring to the mixing region, mixing, and transferring to the reaction region can be repeated multiple times to obtain a large number of resin beads, each of which has been exposed to a series of reactions different from most of the other resin beads.

摘要翻译： 组合化学合成的目的是通过连续化学合成的随机化方法制备大量不同的分子实体。 本发明提供了一种组合容器，其包括彼此相邻的反应区域和混合区域，其可用于实施组合化学合成。 反应区域包括多个反应腔，其中多个不同的化学反应可以在树脂珠上同时进行。 然后将树脂珠转移到混合区域并混合成单组。 然后，将树脂珠转移到反应腔中。 进行反应，转移到混合区域，混合和转移到反应区域的过程可以重复多次，以获得大量的树脂珠粒，其中每一种已经暴露于与大部分 其他树脂珠。

公开(公告)号：US5540826A

公开(公告)日：1996-07-30

申请号：US403982

申请日：1995-03-15

申请人： Milan Bier ,  Terry D. Long ,  Rodolfo Marquez ,  Anthony R. Ford

发明人： Milan Bier ,  Terry D. Long ,  Rodolfo Marquez ,  Anthony R. Ford

IPC分类号： B01D35/06 ,  B01D61/46 ,  B01D63/08 ,  G01N27/447 ,  B01D61/42

CPC分类号： G01N27/44795 ,  B01D35/06 ,  B01D61/46 ,  B01D63/087

摘要： A multi-channel separation device is provided for use in separation technology, particularly in electrophoresis and in isoelectric focusing. The device includes two mating plates, at least one of which contains an array of at least three cavities arranged to define a serpentine fluid pathway through the cavities when the plates are mated. Each of the cavities includes fluid input and output ports to facilitate transfer of process fluid through the cavities. A septum is disposed between the plates for streamlining fluid flow between the input and output ports by restricting but not preventing free fluid flow between adjacent cavities. The device also includes anodic and cathodic compartments containing electrodes for establishing an electric field across the cavities.

摘要翻译： 提供用于分离技术的多通道分离装置，特别是在电泳和等电聚焦中。 该装置包括两个配合板，其中至少一个包含至少三个空腔的阵列，其布置成当板配合时限定通过空腔的蛇形流体路径。 每个空腔包括流体输入和输出端口，以便于工艺流体通过空腔的传送。 隔板设置在板之间，用于通过限制但不防止相邻空腔之间的自由流体流动来简化输入和输出端口之间的流体流动。 该装置还包括阳极和阴极室，其包含用于在空腔之间建立电场的电极。

公开(公告)号：US4897169A

公开(公告)日：1990-01-30

申请号：US333867

申请日：1989-04-03

申请人： Milan Bier ,  Garland E. Twitty

发明人： Milan Bier ,  Garland E. Twitty

IPC分类号： B01D57/02 ,  G01N27/447

CPC分类号： G01N27/44795 ,  B01D57/02 ,  G01N27/44769

摘要： The disclosure is directed to a process and apparatus for isoelectric focusing and isotachophoresis of fluids. Recycling of the process fluid is established in a first direction through an electrophoretic chamber having parallel walls, the electric field being applied in a second direction. Externally of the chamber, the flow of fluid is subdivided into a plurality of closed loops, cooled to dissipate the Joule heat. While maintaining conditions of laminar flow within the chamber, unusual fluid stabilization against convective, electroosmotic and other fluid flow disturbances is obtained if the gap between the parallel walls of the chamber is kept narrow and the fluid flow is rapid, to maximize shear stress at the walls of the chamber and minimize the fluid residence time in the chamber.

摘要翻译： 本公开涉及流体的等电聚焦和等速电泳的方法和装置。 通过具有平行壁的电泳室在第一方向建立工艺流体的再循环，电场沿第二方向施加。 在室的外部，流体流被细分为多个闭合回路，冷却以消散焦耳热。 在保持室内的层流条件的同时，如果室的平行壁之间的间隙保持较窄并且流体流动快速，则可获得不对流，电渗和其它流体流动干扰的异常流体稳定性，以最大化剪切应力 室的壁，并最小化室中的流体停留时间。