公开(公告)号：US20160068897A1

公开(公告)日：2016-03-10

申请号：US14648161

申请日：2013-11-26

Applicant: QVELLA CORPORATION

Inventor： Samad TALEBPOUR ,  Aye Aye KHINE ,  Robert MAASKANT ,  Tino ALAVIE

IPC: C12Q1/68

CPC classification number: B01L3/5021 ,  B01L3/50215 ,  B01L3/5027 ,  B01L7/52 ,  B01L2200/026 ,  B01L2300/046 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/1833 ,  C12Q1/6806 ,  C12Q2523/307 ,  C12Q2527/125 ,  C12Q2527/137

Abstract: Methods and devices are provided for pretreatment of a sample containing microbial cells. In some embodiments, the pretreatment of the sample is performed via the initial selective lysis, within a sample pretreatment vessel, of non-microbial cells (such as blood cells) and the subsequent centrifugal separation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included for collecting the microbial cells adjacent to the liquid interface formed by the cushioning liquid upon centrifugation of the pretreatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the cushioning liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed prior to extraction of the remaining suspension, which provides a “pretreated” sample.

Abstract translation: 提供了用于预处理含有微生物细胞的样品的方法和装置。 在一些实施方案中，样品的预处理通过在样品预处理容器内，非微生物细胞（例如血细胞）中的初始选择性裂解进行，随后离心分离样品以除去所得碎片并将其浓缩 微生物细胞。 可以包括不混溶和致密的缓冲液体，用于在预处理容器离心时收集由缓冲液体形成的液体界面附近的微生物细胞。 在除去大量的上清液后，重新悬浮收集的微生物细胞并重新建立缓冲液体界面，可以除去至少一部分剩余的悬浮液，而基本不除去缓冲液体。 在提取剩余悬浮液之前可以进行一个或多个中间洗涤循环，其提供“预处理”样品。