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    METHODS AND COMPOSITIONS FOR THE SELECTIVE LYSIS OF BLOOD CELLS AND SEPARATION OF MICROBIAL CELLS
    3.
    发明申请
    METHODS AND COMPOSITIONS FOR THE SELECTIVE LYSIS OF BLOOD CELLS AND SEPARATION OF MICROBIAL CELLS 有权

    公开(公告)号：US20210208128A1

    公开(公告)日：2021-07-08

    申请号：US17057088

    申请日：2019-05-24

    申请人： QVELLA CORPORATION

    发明人： Samad TALEBPOUR ,  Aye Aye KHINE ,  Vilcy PARMAR ,  Sukhdev MANKU ,  Alaleh SAMIEI

    IPC分类号： G01N33/49 ,  G01N35/00

    摘要： Methods and compositions are provided for the selective lysis of eukaryotic cells and the separation of microbial cells. Blood cells and/or other eukaryotic cells in a sample, may be selectively lysed by adding, to the sample, a blood lysis reagent including saponin and an alkaline buffer, and optionally sodium polyanethole sulfonate and a non-ionic surfactant, thereby forming a mixture, and agitating the mixture. Microbial cells in the mixture may then be separated, for example, using a separation method such as centrifugation or filtration, and optionally detected or cultured in growth media. Blood lysis reagent compositions are provided that are suitable for preserving the intactness of microbial cells upon mixing with the sample. In example embodiments in which the sample is a blood sample, the blood lysis reagent composition may be selected to avoid or reduce the presence of visible blood debris upon centrifugation or filtration.

    METHODS AND DEVICES FOR ELECTRICAL SAMPLE PREPARATION
    5.
    发明申请
    METHODS AND DEVICES FOR ELECTRICAL SAMPLE PREPARATION 有权
    用于电气样品制备的方法和装置

    公开(公告)号：US20140004501A1

    公开(公告)日：2014-01-02

    申请号：US13750723

    申请日：2013-01-25

    申请人： Qvella Corporation

    发明人： Samad TALEBPOUR ,  Aye Aye KHINE ,  Robert MAASKANT ,  Tino ALAVIE

    IPC分类号： C12N13/00 ,  C12Q1/68 ,  C12M1/42 ,  C12Q3/00

    CPC分类号： C12Q1/6806 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/502753 ,  B01L7/52 ,  B01L2200/0631 ,  B01L2200/143 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/0858 ,  B01L2300/087 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/14 ,  B01L2300/1833 ,  B01L2400/0694 ,  C12M35/02 ,  C12M47/06 ,  C12N1/066 ,  C12N1/08 ,  C12N13/00 ,  C12Q1/686 ,  C12Q3/00

    摘要： Devices and methods are provided for electrically lysing cells and releasing macromolecules from the cells. A microfluidic device is provided that includes a planar channel having a thickness on a submillimeter scale, and including electrodes on its upper and lower inner surfaces. After filling the channel with a liquid, such that the channel contains cells within the liquid, a series of voltage pulses of alternating polarity are applied between the channel electrodes, where the amplitude of the voltage pulses and a pulsewidth of the voltage pulses are effective for causing irreversible electroporation of the cells. The channel is configured to possess thermal properties such that the application of the voltage produces a rapid temperature rise as a result of Joule heating for releasing the macromolecules from the electroplated cells. The channel may also include an internal filter for capturing and concentrating the cells prior to electrical processing.

    摘要翻译： 提供了用于电解裂解细胞并从细胞释放大分子的装置和方法。 提供一种微流体装置，其包括具有亚毫米级厚度的平面通道，并且在其上下表面上包括电极。 在用液体填充通道之后，使得通道在液体内包含单元，在通道电极之间施加一系列交替极性的电压脉冲，其中电压脉冲的幅度和电压脉冲的脉冲宽度对于 引起细胞的不可逆电穿孔。 该通道被配置为具有热性质，使得施加电压由于焦耳加热而导致快速升温，以从电镀细胞释放大分子。 通道还可以包括内部滤波器，用于在电处理之前捕获和集中单元。

    Method for pretreatment of microbial cells
    7.
    发明授权
    Method for pretreatment of microbial cells 有权

    公开(公告)号：US11702647B2

    公开(公告)日：2023-07-18

    申请号：US16881479

    申请日：2020-05-22

    申请人： QVELLA CORPORATION

    发明人： Samad Talebpour ,  Aye Aye Khine ,  Robert Maaskant ,  Tino Alavie

    IPC分类号： C12N15/10 ,  B01L3/00 ,  C12Q1/6806 ,  C12Q1/24 ,  C08F220/28

    CPC分类号： C12N15/1003 ,  B01L3/5021 ,  B01L3/5027 ,  C08F220/286 ,  C12Q1/24 ,  C12Q1/6806 ,  C08G2261/1452

    摘要： Methods and devices are provided for pretreatment of a sample containing microbial cells. In some embodiments, the pretreatment of the sample is performed via the initial selective lysis, within a sample pretreatment vessel, of non-microbial cells (such as blood cells) and the subsequent centrifugal separation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included for collecting the microbial cells adjacent to the liquid interface formed by the cushioning liquid upon centrifugation of the pretreatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the cushioning liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed prior to extraction of the remaining suspension, which provides a “pretreated” sample.

    Apparatus and method for extracting microbial cells
    8.
    发明授权
    Apparatus and method for extracting microbial cells 有权

    公开(公告)号：US10662420B2

    公开(公告)日：2020-05-26

    申请号：US15612091

    申请日：2017-06-02

    申请人： QVELLA CORPORATION

    发明人： Samad Talebpour ,  Aye Aye Khine ,  Robert Maaskant ,  Tino Alavie

    IPC分类号： C12Q1/68 ,  B01L3/00 ,  B01L7/00 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/24

    摘要： Methods and devices are provided for pre-treatment of a sample containing microbial cells. In some embodiments, the pre-treatment of the sample is performed via the initial selective lysis, within a sample pre-treatment vessel, of non-microbial cells, such as blood cells, and the subsequent centrifugal separation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included for collecting the microbial cells adjacent to the liquid interface formed by the cushioning liquid upon centrifugation of the pre-treatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the cushioning liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed prior to extraction of the remaining suspension, which provides a “pretreated” sample.

    Methods of targeted antibiotic susceptibility testing
    9.
    发明授权
    Methods of targeted antibiotic susceptibility testing 有权

    公开(公告)号：US10233483B2

    公开(公告)日：2019-03-19

    申请号：US14901200

    申请日：2014-07-03

    申请人： Qvella Corporation

    发明人： Samad Talebpour ,  Aye Aye Khine ,  Tino Alavie ,  Stephen Wesley Leonard

    IPC分类号： C12Q1/18 ,  C12Q1/68 ,  C12Q1/689

    摘要： Methods are provided for performing antibiotic susceptibility testing based on the detection of RNA, such as tmRNA, from microbial cells after exposure to antibiotics. In some embodiments, aliquots are obtained from a sample, one of which contains a selected antibiotic. The aliquots, which include growth media, are incubated under conditions suitable for microbial growth, and the microbial cells in each aliquot are removed and lysed, and the lysate is subjected to reverse transcription and amplification in infer the effect of the selected antibiotic on the microbial cells. In one embodiment, a sample containing microbial cells is incubated in the presence of a selected antibiotic and a stimulus is provided to induce the production on m RNA within the microbial cells. The microbial cells are subsequently lysed without substantial degradation of the m RNA within the lysate, and the m RNA is detected to determine the effect of the antibiotic on the microbial cells.

    APPARATUS AND METHOD FOR EXTRACTING MICROBIAL CELLS
    10.
    发明申请
    APPARATUS AND METHOD FOR EXTRACTING MICROBIAL CELLS 审中-公开

    公开(公告)号：US20170275612A1

    公开(公告)日：2017-09-28

    申请号：US15612091

    申请日：2017-06-02

    申请人： QVELLA CORPORATION

    发明人： Samad TALEBPOUR ,  Aye Aye KHINE ,  Robert MAASKANT ,  Tino ALAVIE

    IPC分类号： C12N15/10 ,  C12Q1/68

    摘要： Methods and devices are provided for pre-treatment of a sample containing microbial cells. In some embodiments, the pre-treatment of the sample is performed via the initial selective lysis, within a sample pre-treatment vessel, of non-microbial cells, such as blood cells, and the subsequent centrifugal separation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included for collecting the microbial cells adjacent to the liquid interface formed by the cushioning liquid upon centrifugation of the pre-treatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the cushioning liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed prior to extraction of the remaining suspension, which provides a “pretreated” sample.