公开(公告)号：US11150216B2

公开(公告)日：2021-10-19

申请号：US16238451

申请日：2019-01-02

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Roger J. A. Chen ,  Hui Tian ,  J. William Maney, Jr.

IPC: G01N27/447 ,  G01N33/487 ,  B01L3/00 ,  C12Q1/6869

Abstract: A method of analyzing a molecule in a nanopore is disclosed. A voltage is applied across a nanopore that is inserted in a membrane by coupling the nanopore to a voltage source. The nanopore is decoupled from the voltage source. After the decoupling, a rate of decay of the voltage across the nanopore is determined. A molecule in the nanopore is distinguished from other possible molecules based on the determined rate of decay of the voltage across the nanopore.

公开(公告)号：US10670549B2

公开(公告)日：2020-06-02

申请号：US16141658

申请日：2018-09-25

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Bill Maney ,  Hui Tian ,  Santiago Fernandez-Gomez

IPC: G01N27/26 ,  G01N33/487 ,  B82Y35/00

Abstract: A nanopore measurement circuit includes a first analog memory configured to store a first electrical value corresponding to a first measurement sample of a nanopore and a second analog memory configured to store a second electrical value corresponding to a second measurement sample of the nanopore. The nanopore measurement circuit also includes a measurement circuitry configured to provide an output indicating a difference between the first electrical value of the first analog memory and the second electrical value of the second analog memory.

公开(公告)号：US10429375B2

公开(公告)日：2019-10-01

申请号：US16172633

申请日：2018-10-26

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Roger J. A. Chen ,  Hui Tian ,  Santiago Fernandez-Gomez

IPC: G01N27/447 ,  G01N27/22 ,  G01N33/487 ,  C12Q1/6869 ,  B82Y15/00

Abstract: A method of exporting measurements of a nanopore sensor on a nanopore based sequencing chip is disclosed. An electrical characteristic associated with the nanopore sensor is measured. The electrical characteristic associated with the nanopore sensor is processed. A summary for the electrical characteristic and one or more previous electrical characteristics is determined. The summary for the electrical characteristic and the one or more previous electrical characteristics are exported. Determining the summary includes determining that the electrical characteristic and at least a portion of the one or more previous electrical characteristics correspond to a base call event at the nanopore sensor. The summary represents the electrical characteristic and the at least a portion of the one or more previous electrical characteristics.

公开(公告)号：US20220310205A1

公开(公告)日：2022-09-29

申请号：US17806930

申请日：2022-06-14

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Santiago Fernandez-Gomez ,  Hui Tian ,  J. William Maney, JR. ,  Jayalakshmi Rajaraman

IPC: G16B30/00 ,  G01N33/487

Abstract: A system includes a plurality of nanopore cells. Data corresponding to nanopore states of the plurality of nanopore cells is received. The data is analyzed to determine a compressed output size of the data given at least one compression technique. It is determined whether the compressed output size exceeds a data budget. In the event it is determined that the compressed output size exceeds the data budget, the data is modified. The modified data is outputted.

公开(公告)号：US20210396701A1

公开(公告)日：2021-12-23

申请号：US17447048

申请日：2021-09-07

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Roger J.A. Chen ,  J. William Maney, JR. ,  Hui Tian

IPC: G01N27/22 ,  C12Q1/6869 ,  G01N27/447 ,  G01N33/487

Abstract: A method of analyzing a molecule is disclosed. A voltage source is selectively connected to or disconnected from a capacitor using a switch controlled by a reset signal. A charge is stored in a capacitor when the voltage source is connected to the capacitor. The capacitor is discharged through a nanopore in a membrane when the voltage source is disconnected from the capacitor. A duty cycle of the reset signal is determined such that the voltage source and the capacitor is connected for at least a one tenth portion of a reset signal period and disconnected for a remaining portion of the reset signal period, such that a voltage across the nanopore is maintained at a higher level during the portion of the reset signal period in which the connection is maintained than during the remaining portion of the reset signal period in which the connection is not maintained.

公开(公告)号：US10976302B2

公开(公告)日：2021-04-13

申请号：US16868457

申请日：2020-05-06

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Roger J. A. Chen ,  Jennifer Hovis ,  Hui Tian

IPC: G01N33/487

Abstract: A nanopore sequencing device is disclosed. The nanopore sequencing device includes a working electrode. It further includes a dielectric layer, wherein a portion of the dielectric layer is disposed horizontally adjacent to the working electrode and a portion of the dielectric layer is disposed above and covering a portion of the working electrode, and wherein the dielectric layer forms a well having an opening above an uncovered portion of the working electrode. A base surface area of the working electrode is greater than a base surface area of the opening above the uncovered portion of the working electrode.

公开(公告)号：US10935512B2

公开(公告)日：2021-03-02

申请号：US14864400

申请日：2015-09-24

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Santiago Fernandez-Gomez ,  Hui Tian ,  Bill Maney ,  Seung Shin

IPC: G01N27/40 ,  G01N33/487

Abstract: A system includes a circuit configured to detect a voltage corresponding to an electrical measurement of a nanopore. The system also includes a component configured to compare the voltage to another voltage. Based at least in part on the comparison, a one bit indicator is determined. The one bit indicator indicates whether the voltage indicates a change in a state of the nanopore. In the event it is determined that the voltage indicates the change in the state of the nanopore, a multiple bit signal is provided for output.

公开(公告)号：US20200292522A1

公开(公告)日：2020-09-17

申请号：US16892214

申请日：2020-06-03

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Roger J.A. Chen ,  Santiago Fernandez-Gomez ,  Hui Tian

IPC: G01N33/487 ,  C12Q1/6869

Abstract: A method of exporting measurements of a nanopore sensor on a nanopore based sequencing chip is disclosed. An electrical characteristic associated with the nanopore sensor is measured. The electrical characteristic associated with the nanopore sensor is processed. A summary for the electrical characteristic and one or more previous electrical characteristics is determined. The summary for the electrical characteristic and the one or more previous electrical characteristics are exported. Determining the summary includes determining that the electrical characteristic and at least a portion of the one or more previous electrical characteristics correspond to a base call event at the nanopore sensor. The summary represents the electrical characteristic and the at least a portion of the one or more previous electrical characteristics.

公开(公告)号：US20200256818A1

公开(公告)日：2020-08-13

申请号：US16861092

申请日：2020-04-28

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Santiago Fernandez-Gomez ,  Bill Maney ,  Hui Tian

IPC: G01N27/26 ,  B82Y35/00 ,  G01N33/487

Abstract: A nanopore measurement circuit includes a first analog memory configured to store a first electrical value corresponding to a first measurement sample of a nanopore and a second analog memory configured to store a second electrical value corresponding to a second measurement sample of the nanopore. The nanopore measurement circuit also includes a measurement circuitry configured to provide an output indicating a difference between the first electrical value of the first analog memory and the second electrical value of the second analog memory.

公开(公告)号：US20190293624A1

公开(公告)日：2019-09-26

申请号：US16424114

申请日：2019-05-28

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Roger J.A. Chen ,  Jennifer Hovis ,  Hui Tian

IPC: G01N33/487

Abstract: A nanopore sequencing device is disclosed. The nanopore sequencing device includes a working electrode. It further includes a dielectric layer, wherein a portion of the dielectric layer is disposed horizontally adjacent to the working electrode and a portion of the dielectric layer is disposed above and covering a portion of the working electrode, and wherein the dielectric layer forms a well having an opening above an uncovered portion of the working electrode. A base surface area of the working electrode is greater than a base surface area of the opening above the uncovered portion of the working electrode.