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公开(公告)号：US20120178149A1
公开(公告)日：2012-07-12
申请号：US13497757
申请日：2010-09-21
IPC分类号： C12S3/14
摘要： Methods of preparing plant-derived virus like particles (VLPs) are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized VLPs, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived VLPs. Alternatively, VLPs may be obtained from plant or plant matter comprising plant-derived VLPs by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived VLPs are recovered from the filtered fraction.
摘要翻译： 提供了植物来源的病毒样颗粒（VLPs）的制备方法。 该方法可以包括获得植物或包含原生质体定位的VLP的植物物质，从植物或植物物质产生原生质体/原生质体级分和原生质体级分，以及回收载脂蛋白级分。 载脂蛋白级分包含植物来源的VLP。 或者，VLP可以通过使用细胞壁降解酶组合物消化植物物质从包含植物来源的VLP的植物或植物物质获得，以产生消化部分。 将消化的馏分过滤以产生过滤的馏分，从过滤的馏分中回收来自植物的VLP。
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公开(公告)号：US11833200B2
公开(公告)日：2023-12-05
申请号：US13497767
申请日：2010-09-21
IPC分类号： C12N7/00 , C12N15/82 , A61K39/145 , A61K39/12 , A61K39/00
CPC分类号： A61K39/145 , A61K39/12 , C12N7/00 , C12N15/8203 , C12N15/8257 , C12N15/8258 , A61K2039/517 , A61K2039/5258 , C12N2760/16123 , C12N2760/16134
摘要： Methods of preparing plant-derived proteins or suprastructure proteins, are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized proteins, or suprastructure proteins, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived proteins or suprastructure proteins. Alternatively, the proteins, or suprastructure proteins, may be obtained from plant or plant matter comprising plant-derived proteins or suprastructure proteins, by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived proteins or suprastructure proteins, are recovered from the filtered fraction.
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公开(公告)号：US11826419B2
公开(公告)日：2023-11-28
申请号：US13497757
申请日：2010-09-21
IPC分类号： C12N7/00 , C12N15/82 , A61K39/145 , A61K39/12 , A61K39/00
CPC分类号： A61K39/145 , A61K39/12 , C12N7/00 , C12N15/8203 , C12N15/8257 , C12N15/8258 , A61K2039/517 , A61K2039/5258 , C12N2760/16123 , C12N2760/16134
摘要： Methods of preparing plant-derived virus like particles (VLPs) are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized VLPs, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived VLPs. Alternatively, VLPs may be obtained from plant or plant matter comprising plant-derived VLPs by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived VLPs are recovered from the filtered fraction.
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公开(公告)号：US20130067807A1
公开(公告)日：2013-03-21
申请号：US13497767
申请日：2010-09-21
摘要： Methods of preparing plant-derived proteins or suprastructure proteins, are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized proteins, or suprastructure proteins, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived proteins or suprastructure proteins. Alternatively, the proteins, or suprastructure proteins, may be obtained from plant or plant matter comprising plant-derived proteins or suprastructure proteins, by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived proteins or suprastructure proteins, are recovered from the filtered fraction.
摘要翻译： 提供了制备植物衍生蛋白或超基质蛋白的方法。 该方法可以包括获得植物或包含质外体蛋白质定位蛋白质的植物物质或超基质蛋白，从植物或植物物质产生原生质体/原生质体级分和质外体级分，并回收载脂蛋白级分。 载脂蛋白级分包含植物来源的蛋白质或超基因蛋白质。 或者，可以通过使用细胞壁降解酶组合物消化植物物质以产生消化部分，从包含植物衍生的蛋白质或超基质蛋白质的植物或植物物质获得蛋白质或超基质蛋白质。 将消化的馏分过滤以产生过滤的馏分，从过滤的馏分中回收植物来源的蛋白质或超结构蛋白质。
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15.发明申请Bipartite System, Method and Composition for the Constitutive and Inducible Expression of High Levels of Foreign Proteins in Plants 有权用于植物中高水平外源蛋白的组成型和诱导表达的双系统，方法和组成公开(公告)号：US20090181460A1
公开(公告)日：2009-07-16
申请号：US12300922
申请日：2006-05-22
CPC分类号： C12N15/8203 , C12N15/8218 , C12N15/8257 , C12N15/8258
摘要： This invention comprises a combined transgene/virus vector system for the expression of heterologous proteins in plants. While exemplified with respect to the bipartite RNA plant virus, Cowpect mosaic virus (CPMV), other bipartite viral systems may be used to advantage according to the method, system and composition described in detail herein.
摘要翻译： 本发明包括用于在植物中表达异源蛋白质的组合的转基因/病毒载体系统。 尽管关于两部分RNA植物病毒的例证，根据本文详细描述的方法，系统和组合，可以使用考威花叶病毒（CPMV），其他二分之一病毒系统。
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