公开(公告)号：US06399325B1

公开(公告)日：2002-06-04

申请号：US08540650

申请日：1995-10-11

申请人： Shuji Hinuma ,  Ryo Fujii ,  Shoji Fukusumi ,  Tetsuya Ohtaki ,  Masaki Hosoya ,  Kazuhiro Ohgi ,  Haruo Onda

发明人： Shuji Hinuma ,  Ryo Fujii ,  Shoji Fukusumi ,  Tetsuya Ohtaki ,  Masaki Hosoya ,  Kazuhiro Ohgi ,  Haruo Onda

IPC分类号： C12N1512

CPC分类号： C07K14/723

摘要： Galanin receptor proteins, production and use thereof including screening of galanin receptor agonists and antagonists are provided. Galanin receptor proteins, etc. or salts thereof, partial peptides thereof, DNAs coding for the above galanin receptor protein, processes for producing the above receptor protein, methods of screening for a galanin receptor agonist and/or antagonist or screening kits therefor, agonist and/or antagonist compounds or salts thereof obtained by the above screening method or the screening kit, pharmaceutical compositions containing the above compound or its salt, and antibodies against the above receptor protein are provided. It is allowable to efficiently screen a galanin receptor agonist or antagonist by using the galanin receptor protein, the partial peptide thereof, the galanin receptor protein-encoding DNA, the receptor protein-containing cell or its membrane fraction. The pharmaceuticals thus screened or characterized permits various applications including prophylactic and/or therapeutic treatments against a variety of diseases, e.g., stomach ulcer, diabetes, Alzheimer's disease, dementia, etc. and a sedative.

摘要翻译： 提供了Galanin受体蛋白质，其生产和使用，包括甘露糖素受体激动剂和拮抗剂的筛选。 加兰林素受体蛋白等或其盐，其部分肽，编码上述甘丙肽受体蛋白质的DNA，产生上述受体蛋白质的方法，筛选甘丙肽受体激动剂和/或拮抗剂的方法或其筛选试剂盒，激动剂和 /或通过上述筛选方法获得的拮抗剂化合物或其盐或筛选试剂盒，含有上述化合物或其盐的药物组合物和抗上述受体蛋白的抗体。 可以通过使用甘丙肽受体蛋白，其部分肽，编码甘露糖素受体蛋白的DNA，含受体蛋白的细胞或其膜部分来有效筛选甘丙肽受体激动剂或拮抗剂。 如此筛选或表征的药物允许各种应用，包括针对各种疾病（例如胃溃疡，糖尿病，阿尔茨海默病，痴呆等）和镇静剂的预防和/或治疗性治疗。

公开(公告)号：US20080160033A1

公开(公告)日：2008-07-03

申请号：US10542408

申请日：2004-01-15

申请人： Yasuaki Ito ,  Ryo Fujii ,  Shuji Hinuma ,  Shoji Fukusumi ,  Minoru Maruyama

发明人： Yasuaki Ito ,  Ryo Fujii ,  Shuji Hinuma ,  Shoji Fukusumi ,  Minoru Maruyama

IPC分类号： A61K39/395 ,  G01N33/68 ,  C07H21/04 ,  C07K14/705 ,  C12N5/06 ,  A61P5/00 ,  A61P35/00 ,  A61P31/12 ,  A61P3/00 ,  C12P21/02 ,  C12N15/63 ,  A61K31/711 ,  G01N33/566

CPC分类号： C07K14/723 ,  A61K31/711 ,  C07K14/705 ,  G01N2333/726 ,  G01N2500/02

摘要： By using a G protein-coupled receptor protein comprising the same or substantially the same as the amino acid sequence represented by SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 8, or a salt thereof, and a fatty acid, a compound or its salt that changes the binding property of the receptor protein or a salt thereof to the fatty acid can be efficiently screened.

摘要翻译： 通过使用包含与SEQ ID NO：1，SEQ ID NO：3或SEQ ID NO：8表示的氨基酸序列相同或基本相同的G蛋白偶联受体蛋白或其盐，和脂肪酸 可以有效地筛选改变受体蛋白质或其盐与脂肪酸的结合性的化合物或其盐。

公开(公告)号：US6048711A

公开(公告)日：2000-04-11

申请号：US959381

申请日：1997-10-28

申请人： Shuji Hinuma ,  Shoji Fukusumi ,  Yuji Kawamata

发明人： Shuji Hinuma ,  Shoji Fukusumi ,  Yuji Kawamata

IPC分类号： G01N33/53 ,  A61K38/00 ,  A61K39/395 ,  A61K48/00 ,  A61P43/00 ,  C07H21/04 ,  C07K14/705 ,  C07K16/28 ,  C12N1/21 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02 ,  C12P21/08 ,  C12Q1/68 ,  C12R1/19 ,  G01N33/566

CPC分类号： C07K14/705 ,  A61K38/00

摘要： A novel G-protein coupled receptor protein, a partial peptide and their salts are disclosed. DNA encoding the receptor protein, production of the receptor protein, determination of a ligand to the receptor protein, a method for screening for compounds which inhibit ligand binding to the receptor protein, a kit for screening for such compounds are also disclosed. The receptor protein, its partial peptide and their salts are used for screening for candidate compounds of drugs and the like.

摘要翻译： 公开了一种新型的G蛋白偶联受体蛋白，部分肽及其盐。 编码受体蛋白质的DNA，受体蛋白质的产生，受体蛋白质的配体的测定，抑制配体与受体蛋白质结合的化合物的筛选方法，用于筛选这些化合物的试剂盒。 受体蛋白质，其部分肽及其盐用于筛选候选药物化合物等。

公开(公告)号：US6111076A

公开(公告)日：2000-08-29

申请号：US163669

申请日：1998-09-30

申请人： Shoji Fukusumi ,  Shuji Hinuma ,  Ryo Fujii

发明人： Shoji Fukusumi ,  Shuji Hinuma ,  Ryo Fujii

IPC分类号： G01N33/566 ,  A61K38/00 ,  A61K48/00 ,  A61P1/04 ,  A61P3/06 ,  A61P9/08 ,  A61P9/10 ,  A61P13/02 ,  A61P15/00 ,  A61P19/10 ,  A61P25/00 ,  A61P29/00 ,  C07H21/04 ,  C07K14/705 ,  C07K16/28 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02

CPC分类号： C07K14/705 ,  A61K38/00

摘要： Human G-protein coupled receptor polypeptides and DNA (RNA) encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptides for identifying antagonists and agonists to such polypeptides and methods of using the agonists and antagonists therapeutically to treat conditions related to the underexpression and overexpression of the G-protein coupled receptor polypeptides, respectively. Also disclosed are diagnostic methods for detecting a mutation in the G-protein coupled receptor nucleic acid sequences and an altered level of the soluble form of the receptors.

摘要翻译： 公开了人G-蛋白偶联受体多肽和编码此类多肽的DNA（RNA）以及通过重组技术产生此类多肽的方法。 还公开了利用这些多肽来鉴定此类多肽的拮抗剂和激动剂的方法，以及分别在治疗上使用激动剂和拮抗剂治疗与G蛋白偶联受体多肽的低表达和过表达相关的病症的方法。 还公开了用于检测G蛋白偶联受体核酸序列中的突变和受体的可溶形式的改变水平的诊断方法。

公开(公告)号：US5874245A

公开(公告)日：1999-02-23

申请号：US852806

申请日：1997-05-07

申请人： Shoji Fukusumi ,  Shuji Hinuma ,  Ryo Fujii

发明人： Shoji Fukusumi ,  Shuji Hinuma ,  Ryo Fujii

IPC分类号： G01N33/566 ,  A61K38/00 ,  A61K48/00 ,  A61P1/04 ,  A61P3/06 ,  A61P9/08 ,  A61P9/10 ,  A61P13/02 ,  A61P15/00 ,  A61P19/10 ,  A61P25/00 ,  A61P29/00 ,  C07H21/04 ,  C07K14/705 ,  C07K16/28 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02

CPC分类号： C07K14/705 ,  A61K38/00

摘要： Human G-protein coupled receptor polypeptides and DNA (RNA) encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptides for identifying antagonists and agonists to such polypeptides and methods of using the agonists and antagonists therapeutically to treat conditions related to the underexpression and overexpression of the G-protein coupled receptor polypeptides, respectively. Also disclosed are diagnostic methods for detecting a mutation in the G-protein coupled receptor nucleic acid sequences and an altered level of the soluble form of the receptors.

公开(公告)号：US07268212B2

公开(公告)日：2007-09-11

申请号：US10480739

申请日：2002-06-13

申请人： Shuji Hinuma ,  Ryo Fujii ,  Shoji Fukusumi ,  Masaaki Mori ,  Hiromi Yoshida

发明人： Shuji Hinuma ,  Ryo Fujii ,  Shoji Fukusumi ,  Masaaki Mori ,  Hiromi Yoshida

IPC分类号： C07K14/47

CPC分类号： G01N33/566 ,  C07K14/47 ,  C07K14/705 ,  C07K14/723 ,  G01N33/92

摘要： A GPR7 ligand containing the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO:1 wherein the N-terminal amino acid residue may optionally be brominated, is useful in developing a receptor-binding assay system with the use of the GPR7 expression system, in screening a candidate compound for a drug such as an antiobestic, etc.

摘要翻译： 含有与SEQ ID NO：1所示的氨基酸序列相同或基本上相同的氨基酸序列的GPR7配体，其中N-末端氨基酸残基可任选被溴化，可用于开发受体结合测定系统，其中 使用GPR7表达系统，筛选候选化合物用于药物如抗生物等。

公开(公告)号：US07074761B1

公开(公告)日：2006-07-11

申请号：US09207168

申请日：1998-12-07

申请人： Shuji Hinuma ,  Shoji Fukusumi ,  Chieko Kitada

发明人： Shuji Hinuma ,  Shoji Fukusumi ,  Chieko Kitada

IPC分类号： A61K38/10 ,  A61K38/31 ,  C07K14/655 ,  C07K4/12 ,  C07K14/46

CPC分类号： C07K14/575 ,  A61K38/00 ,  C07K14/655 ,  Y10S514/806 ,  Y10S930/16

摘要： The peptides and precursors thereof, inclusive salts thereof, of the present invention are useful as a pharmaceutical composition, for example as therapeutic or prophylactic agents for hormone-producing tumors, acromegaly, gigantism, dementia, gastric ulcer and the like, hormone secretion inhibitors, tumor growth inhibitors, neural activity or sleep modulators, etc. The DNAs coding for the peptides or precursors of the invention are useful as a pharmaceutical composition, for example as agents for the gene therapy or prevention of hormone-producing tumors, acromegaly, gigantism, dementia, gastric ulcer and the like, hormone secretion inhibitors, tumor growth inhibitors, neural activity or sleep modulators, etc. Furthermore, the DNAs coding for the peptides or precursors of the invention are useful as agents for the gene diagnosis of various diseases, for example, hormone-producing tumors, acromegaly, gigantism, dementia, gastric ulcer, etc. The antibodies against the peptides, precursors or salts of the invention can be used for assaying the peptides, precursors or salts of the invention in test solutions. The peptides, precursors or salts of the invention are useful as reagents for screening for compounds, or salts thereof, capable of modifying the binding of the peptides, precursors or salts of the invention to certain receptors.

摘要翻译： 本发明的肽及其前体，包括其的盐可用作药物组合物，例如用于激素产生的肿瘤，肢端肥大症，巨人血症，痴呆，胃溃疡等的治疗或预防剂，激素分泌抑制剂， 肿瘤生长抑制剂，神经活性或睡眠调节剂等。编码本发明的肽或前体的DNA可用作药物组合物，例如作为用于基因治疗或预防激素产生性肿瘤，肢端肥大症，巨人症， 痴呆，胃溃疡等，激素分泌抑制剂，肿瘤生长抑制剂，神经活性或睡眠调节剂等。此外，编码本发明的肽或前体的DNA可用作各种疾病的基因诊断的试剂，用于 例如，产生激素的肿瘤，肢端肥大症，巨人症，痴呆，胃溃疡等。针对肽的抗体 本发明的rs或盐可用于在测试溶液中测定本发明的肽，前体或盐。 本发明的肽，前体或盐可用作筛选能够改变本发明的肽，前体或盐与某些受体的结合的化合物或其盐的试剂。

公开(公告)号：US20100029561A1

公开(公告)日：2010-02-04

申请号：US12449671

申请日：2008-02-19

申请人： Hideki Kizawa ,  Shoji Fukusumi

发明人： Hideki Kizawa ,  Shoji Fukusumi

IPC分类号： A61K38/17 ,  C12Q1/02

CPC分类号： A61K31/7105 ,  A61K31/00 ,  A61K45/06 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/111 ,  C12N2310/14 ,  G01N2800/042

摘要： The present invention provides an insulin sensitizer and a prophylactic/therapeutic agent for diseases involved by sugar metabolic abnormality, comprising a substance that inhibits the expression or activity of CPSF5 protein and/or a substance that inhibits the expression or activity of CPSF6 protein. Provided as the substances are (a) an antisense nucleic acid against a nucleic acid that encodes CPSF5 (or CPSF6), (b) an siRNA against an RNA that encodes CPSF5 (or CPSF6), (c) a nucleic acid capable of producing an siRNA against an RNA that encodes CPSF5 (or CPSF6), and the like. Also provided is a screening method for an insulin resistance ameliorating substance using a cell that produces CPSF5 and/or CPSF6.

摘要翻译： 本发明提供胰岛素敏化剂和糖代谢异常所涉及的疾病的预防/治疗剂，其包含抑制CPSF5蛋白的表达或活性的物质和/或抑制CPSF6蛋白的表达或活性的物质。 提供的物质是（a）针对编码CPSF5（或CPSF6）的核酸的反义核酸，（b）针对编码CPSF5（或CPSF6）的RNA的siRNA，（c）能够产生 针对编码CPSF5（或CPSF6）的RNA的siRNA等。 还提供了使用产生CPSF5和/或CPSF6的细胞的胰岛素抵抗改善物质的筛选方法。