公开(公告)号：US11092607B2

公开(公告)日：2021-08-17

申请号：US15771987

申请日：2016-10-27

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Jellert Gaublomme ,  Aviv Regev

IPC分类号： G01N33/68 ,  G01N33/58 ,  C12Q1/6869 ,  C12Q1/6811 ,  C12Q1/6823 ,  C12Q1/683

摘要： The present invention relates to methods for high multiplex protein or cellular constituent analysis in single cells or single isolated aggregations of cellular constituents. The methods provide for embedding cells or isolated aggregations of cellular constituents in a hydrogel mesh and labeling of cellular constituents with labeling ligands linked to a nucleic acid tag. Cellular constituents can be determined using sequencing methods.

公开(公告)号：US11332736B2

公开(公告)日：2022-05-17

申请号：US16770380

申请日：2018-12-07

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Aviv Regev ,  Orit Rozenblatt-Rosen ,  Jellert Gaublomme ,  Bo Li ,  Kathryn Geiger-Schuller ,  Pratiksha Thakore

IPC分类号： C12N15/10

摘要： The present invention provides methods and tools for analyzing genetic interactions. The subject matter disclosed herein is generally directed to single cell genomics and proteomics. In one embodiment provided is a method of cell and nuclei hashing using sample barcodes. In another embodiment are method of performing genomewide CRISPR perturbation screens.

公开(公告)号：US11841371B2

公开(公告)日：2023-12-12

申请号：US16352355

申请日：2019-03-13

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Aviv Regev ,  Jellert Gaublomme

IPC分类号： G01N33/68 ,  C12N15/10 ,  C12N15/11 ,  C40B40/06

CPC分类号： G01N33/6842 ,  C12N15/1065 ,  C12N15/1093 ,  C12N15/11 ,  C40B40/06 ,  G01N33/6818 ,  C12N15/1093 ,  C12Q2563/179

摘要： The application relates to methods and systems for proteomics and spatial mapping of biomolecules using a next generation sequencing readout to decipher biomolecular and cellular interaction networks. Specifically, disclosed are antenna networks generated by conjugating DNA antennas to proteins. The antennas carry a unique antenna identifier (UAI) sequence that can provide spatial location of the network, as well as biomolecules by transfer of the UAI to reporter oligonucleotides associated with other antennas and biomolecules. The methods and systems are also applicable to single cells.

公开(公告)号：US20210171938A1

公开(公告)日：2021-06-10

申请号：US16770380

申请日：2018-12-07

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Aviv Regev ,  Orit Rozenblatt-Rosen ,  Jellert Gaublomme ,  Bo Li ,  Kathryn Geiger-Schuller

IPC分类号： C12N15/10

摘要： The present invention provides methods and tools for analyzing genetic interactions. The subject matter disclosed herein is generally directed to single cell genomics and proteomics. In one embodiment provided is a method of cell and nuclei hashing using sample barcodes. In another embodiment are method of performing genomewide CRISPR perturbation screens.