公开(公告)号：US07332645B2

公开(公告)日：2008-02-19

申请号：US11027868

申请日：2004-12-30

申请人： Warren J. Leonard ,  Katsutoshi Ozaki ,  Rosanne Spolski

发明人： Warren J. Leonard ,  Katsutoshi Ozaki ,  Rosanne Spolski

IPC分类号： A01K67/00 ,  A01K67/033 ,  A01K67/027 ,  C07H21/02 ,  C07H21/04 ,  C12N15/00

CPC分类号： C07K14/7155 ,  A01K67/0276 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/0381 ,  C07K14/5406 ,  C12N15/8509

摘要： A transgenic mouse is disclosed herein whose somatic and germ cells comprise a disrupted IL-21 receptor gene, the disruption being sufficient to inhibit the binding of IL-21 to an IL-21 receptor, and a disrupted IL-4 gene, the disruption being sufficient to inhibit the production of IL-4 or the binding of IL-4 to the IL-4 receptor. A mouse homozygous for the disrupted IL-21 receptor gene and homozygous for the disrupted IL-4 gene has diminished B cell function. A method is disclosed for altering a B cell activity. The method includes administering a therapeutically effective amount of an agent that interferes with the interaction of IL-21 with an IL-21 receptor, thereby altering the B cell activity. A method is also disclosed for of treating a subject with Job's disorder or atopic disease. A method is also disclosed for treating or preventing an allergic reaction in a subject. A method is also disclosed for treating a subject with an autoimmune or antibody mediated disorder.

摘要翻译： 本文公开了一种转基因小鼠，其体细胞和生殖细胞包含破坏的IL-21受体基因，所述破坏足以抑制IL-21与IL-21受体的结合以及破坏的IL-4基因，所述破坏是 足以抑制IL-4的产生或IL-4与IL-4受体的结合。 对于破坏的IL-21受体基因纯合的小鼠和破坏的IL-4基因是纯合的，具有减少的B细胞功能。 公开了用于改变B细胞活性的方法。 该方法包括施用治疗有效量的干扰IL-21与IL-21受体相互作用的试剂，由此改变B细胞活性。 还公开了用于治疗患有约瑟病或特应性疾病的受试者的方法。 还公开了用于治疗或预防受试者的过敏反应的方法。 还公开了用自身免疫或抗体介导的病症治疗受试者的方法。

公开(公告)号：US08247228B2

公开(公告)日：2012-08-21

申请号：US12126166

申请日：2008-05-23

申请人： Catherine Rachel Ettinger ,  Peter E. Lipsky ,  Warren J. Leonard ,  Rosanne Spolski ,  Herbert C. Morse, III

发明人： Catherine Rachel Ettinger ,  Peter E. Lipsky ,  Warren J. Leonard ,  Rosanne Spolski ,  Herbert C. Morse, III

IPC分类号： C12N5/00 ,  C12N5/07

CPC分类号： G01N33/5052 ,  A61K35/17 ,  A61K38/20 ,  A61K2035/124 ,  A61K2039/5158 ,  C12N5/0635 ,  C12N2501/052 ,  C12N2501/23 ,  C12N2501/50 ,  C12N2501/52 ,  G01N2333/54

摘要： The invention provides a method for promoting differentiation of a mature naïve B cell or a B cell progenitor into a memory B cell or a plasma cell. The method comprises (a) contacting a population of cells comprising a mature naïve B cell or a B cell progenitor with an agent that activates at least one of JAK1, JAK3, STAT3, STAT5A or STAT5B; wherein the population of cells optionally is contacted with an antigen, and (b) isolating the memory B cell or plasma cell.

摘要翻译： 本发明提供了促进成熟初始B细胞或B细胞祖细胞分化成记忆B细胞或浆细胞的方法。 该方法包括（a）使包含成熟初始B细胞或B细胞祖细胞的细胞群与活化JAK1，JAK3，STAT3，STAT5A或STAT5B中的至少一种的药剂接触; 其中细胞群任选地与抗原接触，和（b）分离记忆B细胞或浆细胞。

公开(公告)号：US20080166338A1

公开(公告)日：2008-07-10

申请号：US11958540

申请日：2007-12-18

申请人： Warren J. Leonard ,  Katsutoshi Ozaki ,  Rosanne Spolski ,  Herbert C. Morse ,  Peter E. Lipsky ,  Derry Roopenian

发明人： Warren J. Leonard ,  Katsutoshi Ozaki ,  Rosanne Spolski ,  Herbert C. Morse ,  Peter E. Lipsky ,  Derry Roopenian

IPC分类号： A61K39/395 ,  A61P43/00

CPC分类号： C07K14/7155 ,  A01K67/0276 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/0381 ,  C07K14/5406 ,  C12N15/8509

摘要： A transgenic mouse is disclosed herein whose somatic and germ cells comprise a disrupted IL-21 receptor gene, the disruption being sufficient to inhibit the binding of IL-21 to an IL-21 receptor, and a disrupted IL-4 gene, the disruption being sufficient to inhibit the production of IL-4 or the binding of IL-4 to the IL-4 receptor. A mouse homozygous for the disrupted IL-21 receptor gene and homozygous for the disrupted IL-4 gene has diminished B cell function. A method is disclosed for altering a B cell activity. The method includes administering a therapeutically effective amount of an agent that interferes with the interaction of IL-21 with an IL-21 receptor, thereby altering the B cell activity. A method is also disclosed for of treating a subject with Job's disorder or atopic disease. A method is also disclosed for treating or preventing an allergic reaction in a subject. A method is also disclosed for treating a subject with an autoimmune or antibody mediated disorder.

摘要翻译： 本文公开了一种转基因小鼠，其体细胞和生殖细胞包含破坏的IL-21受体基因，所述破坏足以抑制IL-21与IL-21受体的结合以及破坏的IL-4基因，所述破坏是 足以抑制IL-4的产生或IL-4与IL-4受体的结合。 对于破坏的IL-21受体基因纯合的小鼠和破坏的IL-4基因是纯合的，具有减少的B细胞功能。 公开了用于改变B细胞活性的方法。 该方法包括施用治疗有效量的干扰IL-21与IL-21受体相互作用的试剂，由此改变B细胞活性。 还公开了用于治疗患有约瑟病或特应性疾病的受试者的方法。 还公开了用于治疗或预防受试者的过敏反应的方法。 还公开了用自身免疫或抗体介导的病症治疗受试者的方法。