公开(公告)号：US20130224175A1

公开(公告)日：2013-08-29

申请号：US13818481

申请日：2011-08-24

Applicant: Lodewyk Jacobus Mienie ,  Alberdina Aike Van Dijk ,  Christoffel Petrus Stephanus Badenhorst ,  Rencia Van Der Sluis

Inventor： Lodewyk Jacobus Mienie ,  Alberdina Aike Van Dijk ,  Christoffel Petrus Stephanus Badenhorst ,  Rencia Van Der Sluis

IPC: C12N9/10

CPC classification number: C12N9/1029 ,  C12P21/02 ,  C12Y203/01013

Abstract: This invention relates to a method of producing a recombinant enzyme, more particularly, this invention relates to a method of producing water soluble enzymatically active recombinant glycine N-acyltransferase (GLYAT (E.G. 2.1.3.13)), including the steps of providing a suitable expression host; preparing a vector including a gene for expressing GLYAT in the expression host to form an expression piasmid; transforming the host with the expression piasmid to form an expression system; expressing the GLYAT gene in the expression system; and separating the expressed GLYAT from the expression system.

Abstract translation: 本发明涉及一种生产重组酶的方法，更具体地说，本发明涉及一种生产水溶性酶活性重组甘氨酸N-酰基转移酶（GLYAT（EG2.1.3.13））的方法，包括提供合适表达的步骤 主办; 制备包含用于在表达宿主中表达GLYAT的基因以形成表达piasmid的载体; 用表达式piasmid转换主机以形成表达系统; 在表达系统中表达GLYAT基因; 并将表达的GLYAT与表达系统分离。