公开(公告)号：US20140356852A1

公开(公告)日：2014-12-04

申请号：US14096609

申请日：2013-12-04

Applicant: Alan T. Cheng ,  Ying Zhou

Inventor： Alan T. Cheng ,  Ying Zhou

IPC: C12M1/36

CPC classification number: C12M41/48 ,  C12M21/12 ,  C12M27/00 ,  C12M29/06 ,  C12M29/24 ,  C12M41/32 ,  C12M41/34

Abstract: A method and apparatus for micro-aeration of large scale fermentation systems is provided. The micro-aeration system includes a fermentation reactor, a sparging apparatus, and a micro-aeration gas mixture delivered to the fermentation reactor via the sparging apparatus. The micro-aeration gas mixture is a very low oxygen concentration mixture comprising: (i) an oxygen containing gas; and (ii) an inert carrier gas or reactive carrier gas that is recycled through the fermentation reactor. The gas mixture is introduced to the fermentation reactor at a minimum superficial velocity of about 0.04 m/sec to produce a uniform dispersion of the oxygen throughout the fermentation broth while concurrently mixing the entire fermentation broth. For a reactive gas consuming fermentation process, the amount of reactive carrier gas included as part of the gas mixture exceeds the reaction stoichiometric requirements in order to provide sufficient dispersion and agitation of the broth.

Abstract translation: 提供了一种用于大规模发酵系统微通气的方法和装置。 微曝气系统包括通过喷射装置输送到发酵反应器的发酵反应器，喷射装置和微通气气体混合物。 微通气气体混合物是非常低的氧浓度混合物，其包含：（i）含氧气体; 和（ii）通过发酵反应器再循环的惰性载气或反应性载气。 将气体混合物以约0.04m / sec的最小表观速度引入发酵反应器，以在整个发酵液中同时混合氧气，以均匀分散氧气。 对于反应性气体消耗发酵方法，作为气体混合物的一部分包含的反应性载体气体的量超过反应化学计量要求，以便提供肉汤的足够的分散和搅拌。

公开(公告)号：US20130111931A1

公开(公告)日：2013-05-09

申请号：US13457879

申请日：2012-04-27

Applicant: Nigel J. Grinter ,  Alan T. Cheng ,  Ying Zhou

Inventor： Nigel J. Grinter ,  Alan T. Cheng ,  Ying Zhou

IPC: F25D13/00 ,  F25D3/10

CPC classification number: F25D13/00 ,  A01N1/0252 ,  A01N1/0257 ,  B01L1/025 ,  B01L7/50 ,  B01L2300/0829 ,  B01L2300/14 ,  B01L2300/1838 ,  F25D3/10 ,  F25D3/102 ,  F25D29/001 ,  F25D2600/06

Abstract: A method and system for controlled rate freezing and nucleation of biological materials is provided. The presently disclosed system and method provides the ability to rapidly cool the materials contained in vials or other containers within a cooling unit via forced convective cooling and optionally simultaneous pressure drop using uniform and unidirectional flow of cryogen in proximity to the plurality of vials disposed within a cooling unit. The rapid cooling of the biological materials is achieved by precisely controlling and adjusting the temperature of the cryogen being introduced to the system as well as the chamber pressure as a function of time.

Abstract translation: 提供了一种控制速率冷冻和生物材料成核的方法和系统。 本公开的系统和方法提供了通过强制对流冷却快速冷却冷却单元内的小瓶或其他容器中所含的材料的能力，以及使用均匀且单向的冷冻剂流在靠近设置在一个 冷却单元。 生物材料的快速冷却是通过精确控制和调节引入系统的冷冻剂的温度以及作为时间的函数的室压力来实现的。

公开(公告)号：US20120102982A1

公开(公告)日：2012-05-03

申请号：US13097724

申请日：2011-04-29

Applicant: Ying Zhou ,  Theodore H. Gasteyer, III ,  Nigel J. Grinter ,  Alan T. Cheng ,  Yeu-Chuan Simon Ho ,  Robert R. Sever

Inventor： Ying Zhou ,  Theodore H. Gasteyer, III ,  Nigel J. Grinter ,  Alan T. Cheng ,  Yeu-Chuan Simon Ho ,  Robert R. Sever

IPC: F25D17/04 ,  F25D31/00

CPC classification number: A01N1/0289 ,  A01N1/0257 ,  A01N1/0284 ,  F25D3/102 ,  F25D29/001

Abstract: A method and system for controlled rate freezing and nucleation of materials is provided. The presently disclosed system and method provides the ability to rapidly cool the materials contained in vials or other containers within a cooling unit via forced convective cooling using a laminar and uniform flow of cryogen in proximity to the plurality of vials disposed within the cooling unit. The rapid cooling of the biological materials is achieved by precisely controlling and adjusting the temperature of the cryogen being introduced to the system as a function of time. The presently disclosed methods to systems also provide nucleation control via temperature quench and depressurized nucleation control.

Abstract translation: 提供了控制速率冷冻和成核的方法和系统。 本公开的系统和方法提供了通过强制对流冷却来快速冷却包含在冷却单元内的小瓶或其他容器中的材料的能力，其中使用层流和均匀流动的冷冻剂靠近设置在冷却单元内的多个小瓶。 生物材料的快速冷却是通过精确地控制和调节作为时间的函数引入到系统中的冷冻剂的温度来实现的。 目前公开的系统方法还通过温度骤冷和减压成核控制提供成核控制。

公开(公告)号：US4861352A

公开(公告)日：1989-08-29

申请号：US139462

申请日：1987-12-30

Applicant: Alan T. Cheng

Inventor： Alan T. Cheng

IPC: B01D19/00 ,  B01F3/04 ,  B01F5/04 ,  B01J10/00 ,  B01J19/26 ,  B03D1/00 ,  C02F1/20

CPC classification number: B01F5/045 ,  B01D19/0005 ,  B01J10/002 ,  B01J19/26 ,  C02F1/20 ,  B01F3/04099 ,  B01J2219/00162 ,  B01J2219/00164 ,  Y10S261/75

Abstract: The present invention relates to a method and apparatus for separating a gas, vapor or particulate matter or combinations thereof from a liquid.The liquid is contacted with a stripping gas or vapor which is immiscible in or which has low solubility in the liquid under operating conditions, which stripping gas or vapor is different from the gas or vapor to be separated from the liquid. The contact is achieved in an in-line stripping device which is capable of accelerating at least a portion of the stripping gas or vapor/liquid composition to a linear velocity which is supersonic for the composition.