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5 条记录 (耗时 0.015 秒)

    Vector
    1.
    发明授权
    Vector 失效
    向量

    公开(公告)号：US4784956A

    公开(公告)日：1988-11-15

    申请号：US786474

    申请日：1985-10-11

    申请人： Nigel J. Grinter

    发明人： Nigel J. Grinter

    IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N1/20 ,  C12N1/21 ,  C12N15/00 ,  C12R1/01 ,  C12R1/19 ,  C12N1/00

    CPC分类号： C12N15/00

    摘要： Novel transposable cloning vector and method of using it for the insertion into the chromosome of a recipient Gram-negative bacterium of DNA material foreign to that bacterium. The vector comprises first and second plasmids. The first plasmid carries a transposon (A) a part of whose DNA including the sequence which specifies its transposition function has been removed. The second plasmid carries a fragment of DNA from a transposon (A) including the sequence which specifies its transposition function. The DNA material to be inserted into the chromosome of the recipient bacterium is included in the DNA of transposon (A) carried by the first plasmid. Any suitable transposon may be used as transposon (A) but transposon Tn7 is preferred.

    A METHOD AND SYSTEM FOR CRYOPRESERVATION TO ACHIEVE UNIFORM VIABILITY AND BIOLOGICAL ACTIVITY
    2.
    发明申请
    A METHOD AND SYSTEM FOR CRYOPRESERVATION TO ACHIEVE UNIFORM VIABILITY AND BIOLOGICAL ACTIVITY 审中-公开
    用于实现均匀可行性和生物活性的方法和系统

    公开(公告)号：US20130111931A1

    公开(公告)日：2013-05-09

    申请号：US13457879

    申请日：2012-04-27

    申请人： Nigel J. Grinter ,  Alan T. Cheng ,  Ying Zhou

    发明人： Nigel J. Grinter ,  Alan T. Cheng ,  Ying Zhou

    IPC分类号： F25D13/00 ,  F25D3/10

    CPC分类号： F25D13/00 ,  A01N1/0252 ,  A01N1/0257 ,  B01L1/025 ,  B01L7/50 ,  B01L2300/0829 ,  B01L2300/14 ,  B01L2300/1838 ,  F25D3/10 ,  F25D3/102 ,  F25D29/001 ,  F25D2600/06

    摘要： A method and system for controlled rate freezing and nucleation of biological materials is provided. The presently disclosed system and method provides the ability to rapidly cool the materials contained in vials or other containers within a cooling unit via forced convective cooling and optionally simultaneous pressure drop using uniform and unidirectional flow of cryogen in proximity to the plurality of vials disposed within a cooling unit. The rapid cooling of the biological materials is achieved by precisely controlling and adjusting the temperature of the cryogen being introduced to the system as well as the chamber pressure as a function of time.

    摘要翻译： 提供了一种控制速率冷冻和生物材料成核的方法和系统。 本公开的系统和方法提供了通过强制对流冷却快速冷却冷却单元内的小瓶或其他容器中所含的材料的能力，以及使用均匀且单向的冷冻剂流在靠近设置在一个 冷却单元。 生物材料的快速冷却是通过精确控制和调节引入系统的冷冻剂的温度以及作为时间的函数的室压力来实现的。

    METHOD AND SYSTEM FOR NUCLEATION CONTROL IN A CONTROLLED RATE FREEZER (CRF)
    3.
    发明申请
    METHOD AND SYSTEM FOR NUCLEATION CONTROL IN A CONTROLLED RATE FREEZER (CRF) 有权
    控制速度冻结机（CRF）中核心控制的方法与系统

    公开(公告)号：US20120102982A1

    公开(公告)日：2012-05-03

    申请号：US13097724

    申请日：2011-04-29

    申请人： Ying Zhou ,  Theodore H. Gasteyer, III ,  Nigel J. Grinter ,  Alan T. Cheng ,  Yeu-Chuan Simon Ho ,  Robert R. Sever

    发明人： Ying Zhou ,  Theodore H. Gasteyer, III ,  Nigel J. Grinter ,  Alan T. Cheng ,  Yeu-Chuan Simon Ho ,  Robert R. Sever

    IPC分类号： F25D17/04 ,  F25D31/00

    CPC分类号： A01N1/0289 ,  A01N1/0257 ,  A01N1/0284 ,  F25D3/102 ,  F25D29/001

    摘要： A method and system for controlled rate freezing and nucleation of materials is provided. The presently disclosed system and method provides the ability to rapidly cool the materials contained in vials or other containers within a cooling unit via forced convective cooling using a laminar and uniform flow of cryogen in proximity to the plurality of vials disposed within the cooling unit. The rapid cooling of the biological materials is achieved by precisely controlling and adjusting the temperature of the cryogen being introduced to the system as a function of time. The presently disclosed methods to systems also provide nucleation control via temperature quench and depressurized nucleation control.

    摘要翻译： 提供了控制速率冷冻和成核的方法和系统。 本公开的系统和方法提供了通过强制对流冷却来快速冷却包含在冷却单元内的小瓶或其他容器中的材料的能力，其中使用层流和均匀流动的冷冻剂靠近设置在冷却单元内的多个小瓶。 生物材料的快速冷却是通过精确地控制和调节作为时间的函数引入到系统中的冷冻剂的温度来实现的。 目前公开的系统方法还通过温度骤冷和减压成核控制提供成核控制。

    Transposable cloning vector
    5.
    发明授权
    Transposable cloning vector 失效
    转座克隆载体

    公开(公告)号：US4590162A

    公开(公告)日：1986-05-20

    申请号：US466146

    申请日：1983-02-14

    申请人： Nigel J. Grinter

    发明人： Nigel J. Grinter

    IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N1/20 ,  C12N1/21 ,  C12N15/00 ,  C12R1/01 ,  C12R1/19 ,  C12N1/00

    CPC分类号： C12N15/00

    摘要： Novel transposable cloning vector and method of using it for the insertion into the chromosome of a recipient Gram-negative bacterium of DNA material foreign to that bacterium. The vector comprises first and second plasmids. The first plasmid carries a transposon (A), a part of whose DNA including the sequence which specifies its transposition function has been removed. The second plasmid carries a fragment of DNA from a transposon (A) including the sequence which specifies its transposition function. The DNA material to be inserted into the chromosome of the recipient bacterium is included in the DNA of transposon (A) carried by the first plasmid. Any suitable transposon may be used as transposon (A) but transposon Tn7 is preferred.

    摘要翻译： 新型转座克隆载体及其用于插入到该细菌外源的DNA材料的接受者革兰氏阴性细菌染色体的方法。 载体包含第一和第二质粒。 第一个质粒携带转座子（A），其部分DNA包括指定其转座功能的序列已被去除。 第二个质粒携带转座子（A）的DNA片段，其中包含指定其转座功能的序列。 插入受体细菌的染色体中的DNA材料被包含在由第一质粒携带的转座子（A）的DNA中。 任何合适的转座子可以用作转座子（A），但转座子Tn7是优选的。