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21.发明申请Compositions and Uses for an Alpha-Amylase Polypeptide of Bacillus Species 195 失效芽孢杆菌物种的α-淀粉酶多肽的组成和用途195公开(公告)号：US20120142074A1
公开(公告)日：2012-06-07
申请号：US13312555
申请日：2011-12-06
Applicant: Neelam S. Amin , Melodie Estabrook , Brian E. Jones , Marc Kolkman , Casper Vroemen , Walter Weyler
Inventor： Neelam S. Amin , Melodie Estabrook , Brian E. Jones , Marc Kolkman , Casper Vroemen , Walter Weyler
CPC classification number: C12N9/2417
Abstract: Disclosed herein are compositions comprising an alpha-amylase enzyme obtained from Bacillus sp. no. 195, and methods of using the enzyme to clean surfaces and textiles. Also disclosed are variants of the enzyme with different signal sequences.
Abstract translation: 本文公开了包含从芽孢杆菌属（Bacillus sp。）获得的α-淀粉酶的组合物。 没有。 195，以及使用酶清洁表面和纺织品的方法。 还公开了具有不同信号序列的酶的变体。
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公开(公告)号：US20110281326A1
公开(公告)日：2011-11-17
申请号：US13036749
申请日：2011-02-28
Applicant: George England , Marc Kolkman , Brian Miller , Casper Vroemen
Inventor： George England , Marc Kolkman , Brian Miller , Casper Vroemen
IPC: C12N9/44
CPC classification number: C12Y302/01041 , C12N9/2457
Abstract: The invention relates to novel variants of the enzymatic peptide pullulanase, the gene sequences encoding said novel peptides, expression vectors comprising those gene sequences as well as organisms expressing the novel pullulanase variants. The novel pullulanase variants of the present invention were made empirically by the use of codon-optimization procedures, selective truncation of “wild-type” molecules and through the replacement of selected amino acid residues. Furthermore, the invention relates to the use of these novel pullulanase peptides in the textile, fermentation, food and other industries.
Abstract translation: 本发明涉及酶促支链淀粉酶的新变体，编码所述新肽的基因序列，包含那些基因序列的表达载体以及表达新型支链淀粉酶变体的生物。 通过使用密码子优化程序，选择性截短“野生型”分子和通过置换所选择的氨基酸残基，凭经验提供本发明的新型支链淀粉酶变体。 此外，本发明涉及这些新型支链淀粉酶肽在纺织，发酵，食品和其它工业中的用途。
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公开(公告)号：US07968691B2
公开(公告)日：2011-06-28
申请号：US11841526
申请日：2007-08-20
Applicant: George England , Marc Kolkman , Brian Miller , Casper Vroemen
Inventor： George England , Marc Kolkman , Brian Miller , Casper Vroemen
CPC classification number: C12Y302/01041 , C12N9/2457
Abstract: The invention relates to novel variants of the enzymatic peptide pullulanase, the gene sequences encoding said novel peptides, expression vectors comprising those gene sequences as well as organisms expressing the novel pullulanase variants. The novel pullulanase variants of the present invention were made empirically by the use of codon-optimization procedures, selective truncation of “wild-type” molecules and through the replacement of selected amino acid residues. Furthermore, the invention relates to the use of these novel pullulanase peptides in the textile, fermentation, food and other industries.
Abstract translation: 本发明涉及酶促支链淀粉酶的新变体，编码所述新肽的基因序列，包含那些基因序列的表达载体以及表达新型支链淀粉酶变体的生物。 通过使用密码子优化程序，选择性截短“野生型”分子和通过置换所选择的氨基酸残基，凭经验提供本发明的新型支链淀粉酶变体。 此外，本发明涉及这些新型支链淀粉酶肽在纺织，发酵，食品和其它工业中的用途。
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公开(公告)号：US20110020899A1
公开(公告)日：2011-01-27
申请号：US12681612
申请日：2008-04-08
Applicant: Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
Inventor： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
CPC classification number: C12N5/10
Abstract: The present invention relates to variant glucoamylases wherein the variant has altered properties (e.g., improved thermostability and/or specific activity) compared to a corresponding parent glucoamylase. The present invention also relates to enzyme compositions comprising a variant glucoamylase (e.g., starch hydrolyzing compositions); DNA constructs comprising polynucleotides encoding the variants and methods of producing the variant glucoamylases in host cells
Abstract translation: 本发明涉及变体葡糖淀粉酶，其中与相应的母体葡糖淀粉酶相比，变体具有改变的性质（例如改善的热稳定性和/或比活性）。 本发明还涉及包含变体葡糖淀粉酶（例如淀粉水解组合物）的酶组合物; 包含编码变体的多核苷酸的DNA构建体和在宿主细胞中产生变体葡糖淀粉酶的方法
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