公开(公告)号：US08097444B2

公开(公告)日：2012-01-17

申请号：US12519879

申请日：2007-12-06

Applicant: Neelam S Amin ,  Melodie Estabrook ,  Brian E Jones ,  Marc Kolkman ,  Casper Vroemen ,  Walter Weyler

Inventor： Neelam S Amin ,  Melodie Estabrook ,  Brian E Jones ,  Marc Kolkman ,  Casper Vroemen ,  Walter Weyler

IPC: C12N9/00 ,  C12N9/24 ,  C12N1/20 ,  C12N15/00 ,  C11D3/386 ,  C07H21/04

CPC classification number: C12N9/2417

Abstract: Disclosed herein are compositions comprising an alpha-amylase enzyme obtained from Bacillus sp. no. 195, and methods of using the enzyme to clean surfaces and textiles. Also disclosed are variants of the enzyme with different signal sequences.

Abstract translation: 本文公开了包含从芽孢杆菌属（Bacillus sp。）获得的α-淀粉酶的组合物。 没有。 195，以及使用酶清洁表面和纺织品的方法。 还公开了具有不同信号序列的酶的变体。

公开(公告)号：US20100330647A1

公开(公告)日：2010-12-30

申请号：US12785250

申请日：2010-05-21

Applicant: NEELAM S. AMIN ,  RICHARD R. BOTT ,  MARGUERITE A. CERVIN ,  AYROOKARAN J. POULOSE ,  WALTER WEYLER

Inventor： NEELAM S. AMIN ,  RICHARD R. BOTT ,  MARGUERITE A. CERVIN ,  AYROOKARAN J. POULOSE ,  WALTER WEYLER

IPC: C12N9/18 ,  C07H21/00 ,  C12N15/63 ,  C12N1/21 ,  C12N1/15

CPC classification number: C12N9/16 ,  C11D3/38636

Abstract: The present invention provides methods and compositions comprising at least one perhydrolase enzyme for cleaning and other applications. In some embodiments, the present invention provides methods and compositions for generation of long chain peracids. Certain embodiments of the present invention find particular use in applications involving cleaning, bleaching and disinfecting.

Abstract translation: 本发明提供包含至少一种用于清洁和其它应用的过水解酶的方法和组合物。 在一些实施方案中，本发明提供用于产生长链过酸的方法和组合物。 本发明的某些实施方案特别用于涉及清洁，漂白和消毒的应用中。

公开(公告)号：US20100093028A1

公开(公告)日：2010-04-15

申请号：US12252062

申请日：2008-10-15

Applicant: Neelam S. Amin ,  Katherine D. Collier ,  Melodie Estabrook ,  David A. Estell ,  Bryan P. Fox ,  Scott D. Power ,  Brian F. Schmidt ,  Gudrun Vogtentanz

Inventor： Neelam S. Amin ,  Katherine D. Collier ,  Melodie Estabrook ,  David A. Estell ,  Bryan P. Fox ,  Scott D. Power ,  Brian F. Schmidt ,  Gudrun Vogtentanz

IPC: C12P21/00 ,  C07K14/00 ,  C12N15/74 ,  C12N5/10

CPC classification number: C07K14/811 ,  A61K8/64 ,  A61Q7/02

Abstract: The present invention relates to modified variant Bowman Birk Protease Inhibitor proteins (BBPIs) that comprise peptides that bind target proteins, and that are further modified to have greater protease inhibitory activity and/or be produced at greater yields than the unmodified BBPIs. The invention encompasses polynucleotide constructs and expression vectors containing polynucleotide sequences that encode the modified variant BBPIs, the transformed host cells that express and produce the modified variant BBPIs, the modified variant BBPI proteins, the compositions comprising the modified variant BBPIs, and the methods for making and using the modified variant BBPIs in personal care.

Abstract translation: 本发明涉及包含结合靶蛋白的肽的修饰变体Bowman Birk蛋白酶抑制剂蛋白（BBPI），并且被进一步修饰以具有比未修饰的BBPI更大的蛋白酶抑制活性和/或以更高的产量产生。 本发明包括多核苷酸构建体和含有编码修饰变体BBPI的多核苷酸序列的表达载体，表达和产生修饰变体BBPI的转化宿主细胞，修饰变体BBPI蛋白，包含修饰变体BBPI的组合物和制备方法 并在个人护理中使用修改后的变体BBPI。

公开(公告)号：US20100035787A1

公开(公告)日：2010-02-11

申请号：US12519879

申请日：2007-12-06

Applicant: Neelam S. Amin ,  Melodie Estabrook ,  Brian E. Jones ,  Marc Kolkman ,  Casper Vroemen ,  Walter Weyler

Inventor： Neelam S. Amin ,  Melodie Estabrook ,  Brian E. Jones ,  Marc Kolkman ,  Casper Vroemen ,  Walter Weyler

IPC: C11D3/386 ,  C12N15/56 ,  C12N9/24 ,  C12N15/75 ,  C12N1/21 ,  C11D7/42 ,  C12S9/00 ,  C12N15/70 ,  C12N15/76

CPC classification number: C12N9/2417

Abstract: Disclosed herein are compositions comprising an alpha-amylase enzyme obtained from Bacillus sp. no. 195, and methods of using the enzyme to clean surfaces and textiles. Also disclosed are variants of the enzyme with different signal sequences.

Abstract translation: 本文公开了包含从芽孢杆菌属（Bacillus sp。）获得的α-淀粉酶的组合物。 没有。 195，以及使用酶清洁表面和纺织品的方法。 还公开了具有不同信号序列的酶的变体。

公开(公告)号：US20080145353A1

公开(公告)日：2008-06-19

申请号：US10581014

申请日：2004-12-03

Applicant: Neelam S. Amin ,  Matthew G. Boston ,  Richard R. Bott ,  Marguerite A. Cervin ,  Edward M. Concar ,  Marc E. Gustwiller ,  Brain E. Jones ,  Klaus Liebeton ,  Gregory S. Miracle ,  Hiroshi Oh ,  Ayrookaran J. Poulose ,  Sandra W. Ramer ,  Jeffrey J. Scheibel ,  Walter Weyler ,  Gregory M. Whited

Inventor： Neelam S. Amin ,  Matthew G. Boston ,  Richard R. Bott ,  Marguerite A. Cervin ,  Edward M. Concar ,  Marc E. Gustwiller ,  Brain E. Jones ,  Klaus Liebeton ,  Gregory S. Miracle ,  Hiroshi Oh ,  Ayrookaran J. Poulose ,  Sandra W. Ramer ,  Jeffrey J. Scheibel ,  Walter Weyler ,  Gregory M. Whited

IPC: A01N63/00 ,  C12N9/14 ,  C07K2/00 ,  C07H21/04 ,  C12Q1/68 ,  C12N15/63 ,  C12N1/21 ,  C11D3/20

CPC classification number: A01N63/00 ,  C11D3/38636 ,  C12N9/16 ,  C12N9/50 ,  C12N9/52

Abstract: The present invention provides methods and compositions comprising at least one perhydrolase enzyme for cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions for generation of peracids. The present invention finds particular use in applications involving cleaning, bleaching and disinfecting.

Abstract translation: 本发明提供包含至少一种用于清洁和其它应用的过水解酶的方法和组合物。 在一些特别优选的实施方案中，本发明提供了用于产生过酸的方法和组合物。 本发明特别用于涉及清洗，漂白和消毒的应用中。

公开(公告)号：US20120142074A1

公开(公告)日：2012-06-07

申请号：US13312555

申请日：2011-12-06

Applicant: Neelam S. Amin ,  Melodie Estabrook ,  Brian E. Jones ,  Marc Kolkman ,  Casper Vroemen ,  Walter Weyler

Inventor： Neelam S. Amin ,  Melodie Estabrook ,  Brian E. Jones ,  Marc Kolkman ,  Casper Vroemen ,  Walter Weyler

IPC: C12N9/28 ,  C12N15/63 ,  C11D3/60 ,  D06M16/00 ,  C12S9/00 ,  C11D3/386 ,  C12N15/56 ,  C12N1/21

CPC classification number: C12N9/2417

Abstract: Disclosed herein are compositions comprising an alpha-amylase enzyme obtained from Bacillus sp. no. 195, and methods of using the enzyme to clean surfaces and textiles. Also disclosed are variants of the enzyme with different signal sequences.

Abstract translation: 本文公开了包含从芽孢杆菌属（Bacillus sp。）获得的α-淀粉酶的组合物。 没有。 195，以及使用酶清洁表面和纺织品的方法。 还公开了具有不同信号序列的酶的变体。

公开(公告)号：US07803902B2

公开(公告)日：2010-09-28

申请号：US12252062

申请日：2008-10-15

Applicant: Neelam S. Amin ,  Katherine D. Collier ,  Melodie Estabrook ,  David A. Estell ,  Bryan P. Fox ,  Scott D. Power ,  Brian F. Schmidt ,  Gudrun Vogtentanz

Inventor： Neelam S. Amin ,  Katherine D. Collier ,  Melodie Estabrook ,  David A. Estell ,  Bryan P. Fox ,  Scott D. Power ,  Brian F. Schmidt ,  Gudrun Vogtentanz

IPC: C07K14/00

CPC classification number: C07K14/811 ,  A61K8/64 ,  A61Q7/02

Abstract: The present invention relates to modified variant Bowman Birk Protease Inhibitor proteins (BBPIs) that comprise peptides that bind target proteins, and that are further modified to have greater protease inhibitory activity and/or be produced at greater yields than the unmodified BBPIs. The invention encompasses polynucleotide constructs and expression vectors containing polynucleotide sequences that encode the modified variant BBPIs, the transformed host cells that express and produce the modified variant BBPIs, the modified variant BBPI proteins, the compositions comprising the modified variant BBPIs, and the methods for making and using the modified variant BBPIs in personal care.

Abstract translation: 本发明涉及包含结合靶蛋白的肽的修饰变体Bowman Birk蛋白酶抑制剂蛋白（BBPI），并且被进一步修饰以具有比未修饰的BBPI更大的蛋白酶抑制活性和/或以更高的产量产生。 本发明包括多核苷酸构建体和含有编码修饰变体BBPI的多核苷酸序列的表达载体，表达和产生修饰变体BBPI的转化宿主细胞，修饰变体BBPI蛋白，包含修饰变体BBPI的组合物和制备方法 并在个人护理中使用修改后的变体BBPI。

公开(公告)号：US20100092409A1

公开(公告)日：2010-04-15

申请号：US12252032

申请日：2008-10-15

Applicant: Neelam S. Amin ,  Katherine D. Collier ,  Melodie Estabrook ,  David A. Estell ,  Bryan P. Fox ,  Scott D. Power ,  Brian F. Schmidt ,  Gudrun Vogtentanz

Inventor： Neelam S. Amin ,  Katherine D. Collier ,  Melodie Estabrook ,  David A. Estell ,  Bryan P. Fox ,  Scott D. Power ,  Brian F. Schmidt ,  Gudrun Vogtentanz

IPC: A61Q17/04 ,  C07K14/00 ,  A61K38/10 ,  A61K38/08

CPC classification number: A61K8/64 ,  A61K38/55 ,  A61Q1/02 ,  A61Q1/04 ,  A61Q1/06 ,  A61Q1/10 ,  A61Q3/00 ,  A61Q3/02 ,  A61Q5/006 ,  A61Q5/02 ,  A61Q5/06 ,  A61Q5/12 ,  A61Q17/04 ,  A61Q19/00 ,  A61Q19/002 ,  A61Q19/04 ,  A61Q19/10

Abstract: The present invention relates to compositions that comprise modified variant Bowman Birk Protease Inhibitor proteins (BBPIs). The modified variant BBPIs comprise peptides that bind target proteins, and that are further modified to have greater protease inhibitory activity and/or be produced at greater yields than the unmodified BBPIs. The invention encompasses the compositions comprising the modified variant BBPIs, and the methods for making and using the compositions in personal care.

Abstract translation: 本发明涉及包含修饰的变体Bowman Birk蛋白酶抑制蛋白（BBPI）的组合物。 修饰的变体BBPI包含结合靶蛋白的肽，并且被进一步修饰以具有比未修饰的BBPI更大的蛋白酶抑制活性和/或以更高的产量产生。 本发明包括包含修饰变体BBPI的组合物，以及在个人护理中制备和使用组合物的方法。

公开(公告)号：US09856466B2

公开(公告)日：2018-01-02

申请号：US14115801

申请日：2012-05-04

Applicant: Neelam S. Amin ,  Katherine Augustyn ,  Joshua R. Basler ,  Luis G. Cascao-Pereira ,  Katherine D. Collier ,  Edward M. Concar ,  David A. Estell ,  James T. Kellis, Jr. ,  Euan John Magennis ,  Alexander Pisarchik ,  Ayrookaran J. Poulose ,  Philip Frank Souter ,  Glenn Steven Ward ,  Jian Yao

Inventor： Neelam S. Amin ,  Katherine Augustyn ,  Joshua R. Basler ,  Luis G. Cascao-Pereira ,  Katherine D. Collier ,  Edward M. Concar ,  David A. Estell ,  James T. Kellis, Jr. ,  Euan John Magennis ,  Alexander Pisarchik ,  Ayrookaran J. Poulose ,  Philip Frank Souter ,  Glenn Steven Ward ,  Jian Yao

IPC: C12N9/54 ,  C11D3/386

CPC classification number: C12N9/54 ,  C11D3/386

Abstract: The present invention provides serine protease variants, more specifically subtilisin variants produced there from. Specifically, the present invention provides serine protease variants, more specifically subtilisin variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants, more specifically subtilisin variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants, more specifically subtilisin variants.

公开(公告)号：US08772007B2

公开(公告)日：2014-07-08

申请号：US10581014

申请日：2004-12-03

Applicant: Neelam S. Amin ,  Matthew G. Boston ,  Richard R. Bott ,  Marguerite A. Cervin ,  Edward M. Concar ,  Marc E. Gustwiller ,  Brian E. Jones ,  Klaus Liebeton ,  Gregory S. Miracle ,  Hiroshi Oh ,  Ayrookaran J. Poulose ,  Sandra W. Ramer ,  Jeffrey J. Scheibel ,  Walter Weyler ,  Gregory M. Whited

Inventor： Neelam S. Amin ,  Matthew G. Boston ,  Richard R. Bott ,  Marguerite A. Cervin ,  Edward M. Concar ,  Marc E. Gustwiller ,  Brian E. Jones ,  Klaus Liebeton ,  Gregory S. Miracle ,  Hiroshi Oh ,  Ayrookaran J. Poulose ,  Sandra W. Ramer ,  Jeffrey J. Scheibel ,  Walter Weyler ,  Gregory M. Whited

IPC: C12N9/14 ,  C12N9/18 ,  C12P21/06 ,  C12N1/20 ,  C12N15/00 ,  C11D3/386 ,  C11D3/00 ,  C07H21/04

CPC classification number: A01N63/00 ,  C11D3/38636 ,  C12N9/16 ,  C12N9/50 ,  C12N9/52

Abstract: The present invention provides methods and compositions comprising at least one perhydrolase enzyme for cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions for generation of peracids. The present invention finds particular use in applications involving cleaning, bleaching and disinfecting.

Abstract translation: 本发明提供包含至少一种用于清洁和其它应用的过水解酶的方法和组合物。 在一些特别优选的实施方案中，本发明提供了用于产生过酸的方法和组合物。 本发明特别用于涉及清洗，漂白和消毒的应用中。