公开(公告)号：US20180237950A1

公开(公告)日：2018-08-23

申请号：US15553495

申请日：2016-02-25

Applicant: JumpCode Genomics, Inc. ,  The Scripps Research Institute

Inventor： Keith Brown ,  Daniel R. Salomon ,  Steven Head ,  Azeem Siddique ,  Phillip Ordoukhanian

IPC: C40B40/08

CPC classification number: C40B40/08 ,  C12N15/1093

Abstract: Methods, compositions and kits are provided herein for insertional modification of nucleic acids by, for example transposase-mediated covalent insertion of insertion sequence into a sample nucleic acid molecule. Using sequence of the insertion to direct amplification of adjacent nucleic acid sequence, and using bar codes to map amplified sequence to partitions, one can map sample nucleic acid sequence to single molecules of the nucleic acid sample that are derived directly from the sample nucleic acid molecule.