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公开(公告)号：US07091014B1
公开(公告)日：2006-08-15
申请号：US09423554
申请日：1999-03-11
申请人： Aristos Aristidou , John Londesborough , Merja Penttilä , Peter Richard , Laura Ruohonen , Hans Söderlund , Anita Teleman , Mervi Toivari
发明人： Aristos Aristidou , John Londesborough , Merja Penttilä , Peter Richard , Laura Ruohonen , Hans Söderlund , Anita Teleman , Mervi Toivari
CPC分类号： C12N9/0016 , C12N9/0006 , C12N9/1025 , C12N9/1205 , C12P7/06 , C12P7/18 , C12P7/625 , C12P13/04 , C12P13/08 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to genetic engineering of production microorganisms used in biotechnology to improve their properties so that they produce useful products more efficiently. The microorganisms express at least one enzyme that causes the functional coupling of the oxidation and reduction of substrates by two pyridine nucleotide-linked dehydrogenase reactions with different specificities for the NAD/NADH and NADP/NADPH coenzyme couples and so facilitates the transfer of electrons between the two coenzyme couples through the said substrates. In particular the invention relates to increasing the yields of products such as ethanol or amino acids from carbon and nitrogen sources such as biomass comprising hexoes, pentoses or their polymers.
摘要翻译： 本发明涉及用于生物技术的生产微生物的基因工程，以改善其性质，使得它们更有效地生产有用的产品。 微生物表达至少一种酶，其通过与NAD / NADH和NADP / NADPH辅酶偶合的不同特异性的两个吡啶核苷酸连接的脱氢酶反应引起底物的氧化和还原的功能偶联，并且因此促进电子在 两种辅酶对通过所述底物。 特别地，本发明涉及从碳和氮源例如包含己糖，戊糖或其聚合物的生物质中提高产物如乙醇或氨基酸的产率。
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公开(公告)号：US20060110810A1
公开(公告)日：2006-05-25
申请号：US11263496
申请日：2005-10-31
CPC分类号： C12N9/0006 , C12N15/81 , C12P7/56
摘要： This invention provides biocatalysts that are recombinant yeast cells comprising recombinant expression vectors encoding heterologous lactate dehydrogenase genes for producing lactate.
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