公开(公告)号：US08828707B2

公开(公告)日：2014-09-09

申请号：US13078128

申请日：2011-04-01

申请人： Pim Van Hoek ,  Aristos Aristidou ,  Brian Rush

发明人： Pim Van Hoek ,  Aristos Aristidou ,  Brian Rush

IPC分类号： C12P21/00 ,  C12N15/81 ,  C12N9/04 ,  C12N1/16 ,  C12P7/56 ,  C12P7/62 ,  C12N1/15

CPC分类号： C12P7/56 ,  C12N1/16 ,  C12N9/0006 ,  C12N15/815 ,  C12P7/625 ,  C12Q1/02 ,  G01N2333/39 ,  Y02P20/52

摘要： Specific oxygen uptake (OUR) is used as a process control parameter in fermentation processes. OUR is determined during at least the production phase of a fermentation process, and process parameters are adjusted to maintain the OUR within desired ranges. The invention is particularly applicable when the fermentation is conducted using a microorganism having a natural PDC pathway that has been disrupted so that it no longer functions. Microorganisms of this sort often produce poorly under strictly anaerobic conditions. Microaeration controlled by monitoring OUR allows the performance of the microorganism to be optimized.

摘要翻译： 比氧吸收（OUR）用作发酵过程中的过程控制参数。 在至少在发酵过程的生产阶段确定OUR，并调整工艺参数以将OUR保持在所需范围内。 当使用已经被破坏的天然PDC途径的微生物进行发酵以使其不再起作用时，本发明特别适用。 这种微生物在严格的无氧条件下往往产生不良。 通过监测OUR控制的微量曝气允许优化微生物的性能。

公开(公告)号：US08623633B2

公开(公告)日：2014-01-07

申请号：US13107882

申请日：2011-05-14

申请人： Vineet Rajgarhia ,  Kari Koivuranta ,  Merja Penttilä ,  Marja Ilmen ,  Pirkko Suominen ,  Aristos Aristidou ,  Christopher Kenneth Miller ,  Stacey Olson ,  Laura Ruohonen

发明人： Vineet Rajgarhia ,  Kari Koivuranta ,  Merja Penttilä ,  Marja Ilmen ,  Pirkko Suominen ,  Aristos Aristidou ,  Christopher Kenneth Miller ,  Stacey Olson ,  Laura Ruohonen

IPC分类号： C12N1/00

CPC分类号： C12P7/14 ,  C12N1/16 ,  C12N9/0006 ,  C12N9/1205 ,  C12N9/1217 ,  C12N9/248 ,  C12N9/88 ,  C12N9/92 ,  C12N15/81 ,  C12N15/815 ,  C12P7/06 ,  C12P7/56 ,  C12Y101/01009 ,  C12Y207/01017 ,  C12Y207/02003 ,  C12Y302/01037 ,  C12Y401/01001 ,  C12Y501/03002 ,  C12Y503/01005 ,  Y02E50/17 ,  Y02P20/52

摘要： Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.

摘要翻译： 酵母细胞用外源木糖异构酶基因转化。 另外的遗传修饰增强转化细胞将木糖发酵成乙醇或其它所需发酵产物的能力。 这些修饰包括非特异性或特异性醛糖还原酶基因的缺失，木糖醇脱氢酶基因的缺失和/或木酮糖激酶的过表达。

公开(公告)号：US20120129230A1

公开(公告)日：2012-05-24

申请号：US13316044

申请日：2011-12-09

申请人： Pirkko Suominen ,  Aristos Aristidou ,  Merja Penttila ,  Marja Ilmen ,  Laura Ruohonen ,  Kari Koivuranta ,  Kevin Roberg-Perez

发明人： Pirkko Suominen ,  Aristos Aristidou ,  Merja Penttila ,  Marja Ilmen ,  Laura Ruohonen ,  Kari Koivuranta ,  Kevin Roberg-Perez

IPC分类号： C12P7/56 ,  C12P17/06 ,  C12P7/62 ,  C12N1/19

CPC分类号： C12P7/56 ,  C12N9/0006

摘要： Cells of the species Issatchenkia orientalis and closely related yeast species are transformed with a vector to introduce an exogenous lactate dehydrogenase gene. The cells produce lactic acid efficiently and are resistant at low pH, high lactate titer conditions.

摘要翻译： 用载体转化东方蓟马和密切相关酵母菌种的细胞，引入外源性乳酸脱氢酶基因。 细胞有效地产生乳酸并且在低pH，高乳酸盐滴度条件下具有抗性。

公开(公告)号：US08097448B2

公开(公告)日：2012-01-17

申请号：US11921161

申请日：2006-05-30

申请人： Pirkko Suominen ,  Aristos Aristidou ,  Merja Pentilla ,  Marja Ilmen ,  Laura Ruohonen ,  Kari Koivuranta ,  Kevin Roberg-Perez

发明人： Pirkko Suominen ,  Aristos Aristidou ,  Merja Pentilla ,  Marja Ilmen ,  Laura Ruohonen ,  Kari Koivuranta ,  Kevin Roberg-Perez

IPC分类号： C12N1/00 ,  C12N15/00 ,  C12N15/09

CPC分类号： C12P7/56 ,  C12N9/0006

摘要： Cells of the species Issatchenkia orientalis and closely related yeast species are transformed with a vector to introduce an exogenous lactate dehydrogenase gene. The cells produce lactic acid efficiently and are resistant at low pH, high lactate titer conditions.

摘要翻译： 用载体转化东方蓟马和密切相关酵母菌种的细胞，引入外源性乳酸脱氢酶基因。 细胞有效地产生乳酸并且在低pH，高乳酸盐滴度条件下具有抗性。

公开(公告)号：US20070148748A1

公开(公告)日：2007-06-28

申请号：US11707188

申请日：2007-02-14

申请人： Pim Hoek ,  Aristos Aristidou ,  Brian Rush

发明人： Pim Hoek ,  Aristos Aristidou ,  Brian Rush

IPC分类号： C12P7/56 ,  C12N1/18

CPC分类号： C12P7/56 ,  C12N1/16 ,  C12N9/0006 ,  C12N15/815 ,  C12P7/625 ,  C12Q1/02 ,  G01N2333/39 ,  Y02P20/52

摘要： Specific oxygen uptake (OUR) is used as a process control parameter in fermentation processes. OUR is determined during at least the production phase of a fermentation process, and process parameters are adjusted to maintain the OUR within desired ranges. The invention is particularly applicable when the fermentation is conducted using a microorganism having a natural PDC pathway that has been disrupted so that it no longer functions. Microorganisms of this sort often produce poorly under strictly anaerobic conditions. Microaeration controlled by monitoring OUR allows the performance of the microorganism to be optimized.

摘要翻译： 比氧吸收（OUR）用作发酵过程中的过程控制参数。 在至少在发酵过程的生产阶段确定OUR，并调整工艺参数以将OUR保持在所需范围内。 当使用已经被破坏的天然PDC途径的微生物进行发酵以使其不再起作用时，本发明特别适用。 这种微生物在严格的无氧条件下往往产生不良。 通过监测OUR控制的微量曝气允许优化微生物的性能。

公开(公告)号：US20060234364A1

公开(公告)日：2006-10-19

申请号：US10554887

申请日：2004-05-03

申请人： Vineet Rajgarhia ,  Kari Koivuranta ,  Merja Penttilä ,  Marja Ilmen ,  Pirkko Suominen ,  Aristos Aristidou ,  Christopher Miller ,  Stacey Olson ,  Laura Ruohonen

发明人： Vineet Rajgarhia ,  Kari Koivuranta ,  Merja Penttilä ,  Marja Ilmen ,  Pirkko Suominen ,  Aristos Aristidou ,  Christopher Miller ,  Stacey Olson ,  Laura Ruohonen

IPC分类号： C12P7/06 ,  C12N9/12 ,  C12N15/74 ,  C12N1/18

CPC分类号： C12P7/14 ,  C12N1/16 ,  C12N9/0006 ,  C12N9/1205 ,  C12N9/1217 ,  C12N9/248 ,  C12N9/88 ,  C12N9/92 ,  C12N15/81 ,  C12N15/815 ,  C12P7/06 ,  C12P7/56 ,  C12Y101/01009 ,  C12Y207/01017 ,  C12Y207/02003 ,  C12Y302/01037 ,  C12Y401/01001 ,  C12Y501/03002 ,  C12Y503/01005 ,  Y02E50/17 ,  Y02P20/52

摘要： Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications', include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.

摘要翻译： 酵母细胞用外源木糖异构酶基因转化。 另外的遗传修饰增强转化细胞将木糖发酵成乙醇或其它所需发酵产物的能力。 这些修饰包括非特异性或特异性醛糖还原酶基因的缺失，木糖醇脱氢酶基因的缺失和/或木酮糖激酶的过表达。

公开(公告)号：US07091014B1

公开(公告)日：2006-08-15

申请号：US09423554

申请日：1999-03-11

申请人： Aristos Aristidou ,  John Londesborough ,  Merja Penttilä ,  Peter Richard ,  Laura Ruohonen ,  Hans Söderlund ,  Anita Teleman ,  Mervi Toivari

发明人： Aristos Aristidou ,  John Londesborough ,  Merja Penttilä ,  Peter Richard ,  Laura Ruohonen ,  Hans Söderlund ,  Anita Teleman ,  Mervi Toivari

IPC分类号： C12P7/06 ,  C12N1/14

CPC分类号： C12N9/0016 ,  C12N9/0006 ,  C12N9/1025 ,  C12N9/1205 ,  C12P7/06 ,  C12P7/18 ,  C12P7/625 ,  C12P13/04 ,  C12P13/08 ,  Y02E50/16 ,  Y02E50/17

摘要： The present invention relates to genetic engineering of production microorganisms used in biotechnology to improve their properties so that they produce useful products more efficiently. The microorganisms express at least one enzyme that causes the functional coupling of the oxidation and reduction of substrates by two pyridine nucleotide-linked dehydrogenase reactions with different specificities for the NAD/NADH and NADP/NADPH coenzyme couples and so facilitates the transfer of electrons between the two coenzyme couples through the said substrates. In particular the invention relates to increasing the yields of products such as ethanol or amino acids from carbon and nitrogen sources such as biomass comprising hexoes, pentoses or their polymers.

摘要翻译： 本发明涉及用于生物技术的生产微生物的基因工程，以改善其性质，使得它们更有效地生产有用的产品。 微生物表达至少一种酶，其通过与NAD / NADH和NADP / NADPH辅酶偶合的不同特异性的两个吡啶核苷酸连接的脱氢酶反应引起底物的氧化和还原的功能偶联，并且因此促进电子在 两种辅酶对通过所述底物。 特别地，本发明涉及从碳和氮源例如包含己糖，戊糖或其聚合物的生物质中提高产物如乙醇或氨基酸的产率。

公开(公告)号：US20120028323A1

公开(公告)日：2012-02-02

申请号：US13267771

申请日：2011-10-06

申请人： Reid M. Renny Feldman ,  Uvini Gunawardena ,  Jun Urano ,  Peter Meinhold ,  Aristos Aristidou ,  Catherine Asleson Dundon ,  Christopher Smith

发明人： Reid M. Renny Feldman ,  Uvini Gunawardena ,  Jun Urano ,  Peter Meinhold ,  Aristos Aristidou ,  Catherine Asleson Dundon ,  Christopher Smith

IPC分类号： C12P7/16 ,  C12N1/19

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1022 ,  C12N9/88 ,  C12N9/90 ,  C12N15/81 ,  C12Y101/01001 ,  C12Y101/01008 ,  C12Y101/01086 ,  C12Y101/05003 ,  C12Y202/01006 ,  C12Y401/01001 ,  C12Y401/01074 ,  C12Y402/01009 ,  Y02E50/10

摘要： The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

摘要翻译： 本发明提供包含产生异丁醇的代谢途径的重组微生物和使用所述重组微生物产生异丁醇的方法。 在本发明的各个方面，重组微生物可以包含导致丙酮酸脱羧酶和/或甘油-3-磷酸脱氢酶活性降低的修饰。 在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物 ，和非发酵酵母微生物。

公开(公告)号：US20110201090A1

公开(公告)日：2011-08-18

申请号：US13025801

申请日：2011-02-11

申请人： Thomas Buelter ,  Andrew Hawkins ,  Stephanie Porter-Scheinman ,  Peter Meinhold ,  Catherine Asleson Dundon ,  Aristos Aristidou ,  Jun Urano ,  Doug Lies ,  Matthew Peters ,  Melissa Dey ,  Justas Jancauskas ,  Kent Evans ,  Julie Kelly ,  Ruth Berry

发明人： Thomas Buelter ,  Andrew Hawkins ,  Stephanie Porter-Scheinman ,  Peter Meinhold ,  Catherine Asleson Dundon ,  Aristos Aristidou ,  Jun Urano ,  Doug Lies ,  Matthew Peters ,  Melissa Dey ,  Justas Jancauskas ,  Kent Evans ,  Julie Kelly ,  Ruth Berry

IPC分类号： C12N1/00

CPC分类号： C12N15/81 ,  C12N9/0006 ,  C12P7/16 ,  C12Y101/01 ,  C12Y102/01005 ,  Y02E50/10 ,  Y02P20/52

摘要： The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

摘要翻译： 本发明涉及包含生物合成途径的重组微生物和使用所述重组微生物产生各种有益代谢物的方法。 在本发明的各个方面，重组微生物可以进一步包含一种或多种修饰，导致还原或消除3个酮酸（例如乙酰乳酸和2-乙酰-2-羟基丁酸酯）和/或醛衍生的副产物 。 在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物 ，和非发酵酵母微生物。

公开(公告)号：US20110076733A1

公开(公告)日：2011-03-31

申请号：US12855276

申请日：2010-08-12

申请人： Jun Urano ,  Catherine Asleson Dundon ,  Peter Meinhold ,  Reid M. Renny Feldman ,  Aristos Aristidou ,  Andrew Hawkins ,  Thomas Buelter ,  Matthew Peters ,  Doug Lies ,  Stephanie Porter-Scheinman ,  Ruth Berry ,  Ishmeet Kalra

发明人： Jun Urano ,  Catherine Asleson Dundon ,  Peter Meinhold ,  Reid M. Renny Feldman ,  Aristos Aristidou ,  Andrew Hawkins ,  Thomas Buelter ,  Matthew Peters ,  Doug Lies ,  Stephanie Porter-Scheinman ,  Ruth Berry ,  Ishmeet Kalra

IPC分类号： C12P7/16 ,  C12N1/00 ,  C12N1/21 ,  C12N1/19

CPC分类号： C12N15/81 ,  C12N9/0006 ,  C12N9/1022 ,  C12N9/88 ,  C12P7/16 ,  Y02E50/10

摘要： The present invention provides recombinant microorganisms comprising isobutanol producing metabolic pathway with at least one isobutanol pathway enzyme localized in the cytosol, wherein said recombinant microorganism is selected to produce isobutanol from a carbon source. Methods of using said recombinant microorganisms to produce isobutanol are also provided. In various aspects of the invention, the recombinant microorganisms may comprise a cytosolically active isobutanol pathway enzymes. In some embodiments, the invention provides mutated, modified, and/or chimeric isobutanol pathway enzymes with cytosolic activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.