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公开(公告)号：US07091014B1
公开(公告)日：2006-08-15
申请号：US09423554
申请日：1999-03-11
申请人： Aristos Aristidou , John Londesborough , Merja Penttilä , Peter Richard , Laura Ruohonen , Hans Söderlund , Anita Teleman , Mervi Toivari
发明人： Aristos Aristidou , John Londesborough , Merja Penttilä , Peter Richard , Laura Ruohonen , Hans Söderlund , Anita Teleman , Mervi Toivari
CPC分类号： C12N9/0016 , C12N9/0006 , C12N9/1025 , C12N9/1205 , C12P7/06 , C12P7/18 , C12P7/625 , C12P13/04 , C12P13/08 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to genetic engineering of production microorganisms used in biotechnology to improve their properties so that they produce useful products more efficiently. The microorganisms express at least one enzyme that causes the functional coupling of the oxidation and reduction of substrates by two pyridine nucleotide-linked dehydrogenase reactions with different specificities for the NAD/NADH and NADP/NADPH coenzyme couples and so facilitates the transfer of electrons between the two coenzyme couples through the said substrates. In particular the invention relates to increasing the yields of products such as ethanol or amino acids from carbon and nitrogen sources such as biomass comprising hexoes, pentoses or their polymers.
摘要翻译： 本发明涉及用于生物技术的生产微生物的基因工程，以改善其性质，使得它们更有效地生产有用的产品。 微生物表达至少一种酶，其通过与NAD / NADH和NADP / NADPH辅酶偶合的不同特异性的两个吡啶核苷酸连接的脱氢酶反应引起底物的氧化和还原的功能偶联，并且因此促进电子在 两种辅酶对通过所述底物。 特别地，本发明涉及从碳和氮源例如包含己糖，戊糖或其聚合物的生物质中提高产物如乙醇或氨基酸的产率。
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公开(公告)号：US20080038779A1
公开(公告)日：2008-02-14
申请号：US11751855
申请日：2007-05-22
申请人： Andrei Miasnikov , Heikki Ojamo , Mira Povelainen , Hakan Gros , Mervi Toivari , Peter Richard , Laura Ruohonen , Kari Koivuranta , John Londesborough , Aristos Aristidou , Merja Penttila , Claire Plazanet-Menut , Josef Deutscher
发明人： Andrei Miasnikov , Heikki Ojamo , Mira Povelainen , Hakan Gros , Mervi Toivari , Peter Richard , Laura Ruohonen , Kari Koivuranta , John Londesborough , Aristos Aristidou , Merja Penttila , Claire Plazanet-Menut , Josef Deutscher
IPC分类号： C12P19/00
CPC分类号： C12P7/18
摘要： The invention relates to the methods of manufacturing five-carbon sugars and sugar alcohols as well as other compounds derived from pentose-phosphate pathway from readily available substrates such a hexoses using metabolically engineered microbial hosts.
摘要翻译： 本发明涉及制备五碳糖和糖醇的方法以及衍生自可利用底物的戊糖磷酸途径的其它化合物，例如使用代谢工程的微生物宿主的己糖。
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公开(公告)号：US07226761B2
公开(公告)日：2007-06-05
申请号：US09908744
申请日：2001-07-20
申请人： Andrei Miasnikov , Heikki Ojamo , Mira Povelainen , Hakan Gros , Mervi Toivari , Peter Richard , Laura Ruohonen , Kari Koivuranta , John Londesborough , Aristos Aristidou , Merja Penttila , Claire Plazanet-Menut , Josef Deutscher
发明人： Andrei Miasnikov , Heikki Ojamo , Mira Povelainen , Hakan Gros , Mervi Toivari , Peter Richard , Laura Ruohonen , Kari Koivuranta , John Londesborough , Aristos Aristidou , Merja Penttila , Claire Plazanet-Menut , Josef Deutscher
CPC分类号： C12N9/0006 , C07H1/00 , C07H3/02 , C12N9/1022 , C12N9/1241 , C12N9/90 , C12P7/18
摘要： The invention relates to the methods of manufacturing five-carbon sugars and sugar alcohols as well as other compounds derived from pentose-phosphate pathway from readily available substrates such a hexoses using metabolically engineered microbial hosts.
摘要翻译： 本发明涉及制备五碳糖和糖醇的方法以及衍生自可利用底物的戊糖磷酸途径的其它化合物，例如使用代谢工程的微生物宿主的己糖。
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公开(公告)号：US09315834B2
公开(公告)日：2016-04-19
申请号：US13256559
申请日：2010-03-17
申请人： Peter Richard , Marilyn Wiebe , Mervi Toivari , Dominik Mojzita , Laura Ruohonen , Merja Penttilä
发明人： Peter Richard , Marilyn Wiebe , Mervi Toivari , Dominik Mojzita , Laura Ruohonen , Merja Penttilä
CPC分类号： C12P7/58 , C12N9/0006
摘要： Provided is a method for producing xylonic acid from xylose with a recombinant fungal strain that is genetically modified to express a xylose dehydrogenase gene, which is able to convert xylose to xylonolactone, which is spontaneously or enzymatically hydrolyzed to xylonic acid. The xylonic acid is excreted outside the host cell. Xylonate production may be coupled with xylitol production. Alternatively, if xylitol production is not desired, its production is reduced by removing the aldose reductase (or specific xylose reductase) enzyme, which converts xylose to xylitol. Expression of a heterologous lactonase encoding gene may result in higher acid concentrations. The method is suitable for producing xylonic acid from a hemicellulose hydrolysate such as hydrolyzed lignocellulosic plant biomass.
摘要翻译： 本发明提供了一种从木糖中生产木糖的方法，该重组真菌菌株被遗传修饰以表达木糖脱氢酶基因，其能够将木糖转化为己内酰胺，该木糖自发或酶促水解成木糖酸。 木糖酸在宿主细胞外排出。 木糖苷生产可能与木糖醇生产相结合。 或者，如果不需要木糖醇生产，则通过除去将木糖转化为木糖醇的醛糖还原酶（或特异性木糖还原酶）酶来降低其产生。 异源内含子编码基因的表达可能导致较高的酸浓度。 该方法适用于从半纤维素水解产物如水解木质纤维素植物生物质生产木糖酸。
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公开(公告)号：US20120005788A1
公开(公告)日：2012-01-05
申请号：US13256559
申请日：2010-03-17
申请人： Peter Richard , Marilyn Wiebe , Mervi Toivari , Dominik Mojzita , Laura Ruohonen , Merja Penttilä
发明人： Peter Richard , Marilyn Wiebe , Mervi Toivari , Dominik Mojzita , Laura Ruohonen , Merja Penttilä
CPC分类号： C12P7/58 , C12N9/0006
摘要： Provided is a method for producing xylonic acid from xylose with a recombinant fungal strain that is genetically modified to express a xylose dehydrogenase gene, which is able to convert xylose to xylonolactone, which is spontaneously or enzymatically hydrolysed to xylonic acid. The xylonic acid is excreted outside the host cell. Xylonate production may be coupled with xylitol production. Alternatively, if xylitol production is not desired, its production is reduced by removing the aldose reductase (or specific xylose reductase) enzyme, which converts xylose to xylitol. Expression of a heterologous lactonase encoding gene may result in higher acid concentrations. The method is suitable for producing xylonic acid from a hemicellulose hydrolysate such as hydrolysed lignocellulosic plant biomass.
摘要翻译： 本发明提供了一种从木糖中生产木糖的方法，该重组真菌菌株被遗传修饰以表达木糖脱氢酶基因，其能够将木糖转化为己内酰胺，该木糖自发或酶促水解成木糖酸。 木糖酸在宿主细胞外排出。 木糖苷生产可能与木糖醇生产相结合。 或者，如果不需要木糖醇生产，则通过除去将木糖转化为木糖醇的醛糖还原酶（或特异性木糖还原酶）酶来降低其产生。 异源内含子编码基因的表达可能导致较高的酸浓度。 该方法适用于从半纤维素水解产物如水解木质纤维素植物生物质中生产木糖酸。
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公开(公告)号：US09238826B2
公开(公告)日：2016-01-19
申请号：US13883995
申请日：2011-11-14
摘要： The present invention relates to a method for producing terpenes in fungi, wherein a terpene biosynthetic gene cluster having terpene biosynthetic genes and regulatory regions operably linked to said genes is activated. The invention relates also to a terpene biosynthetic gene duster and regulatory regions of such terpene biosynthetic gene cluster usable is production of terpenes, use of regulator for regulating the terpene production and use of Aspergillus nidulans FGSC A4 for producing terpenes. The method of invention provides higher yields of enriched terpene product without essential amount of side-products.
摘要翻译： 本发明涉及一种在真菌中生产萜烯的方法，其中具有萜烯生物合成基因的萜烯生物合成基因簇和与所述基因可操作地连接的调节区被激活。 本发明还涉及萜烯生物合成基因除尘器和这种萜烯生物合成基因簇的调节区域，可用于生产萜烯，使用调节剂来调节萜烯生产和使用构巢曲霉FGSC A4来生产萜烯。 本发明的方法提供了较高收率的富含萜烯产物，而没有必需量的副产物。
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公开(公告)号：US20140045238A1
公开(公告)日：2014-02-13
申请号：US13883995
申请日：2011-11-14
IPC分类号： C12P5/00
摘要： The present invention relates to a method for producing terpenes in fungi, wherein a terpene biosynthetic gene cluster having terpene biosynthetic genes and regulatory regions operably linked to said genes is activated. The invention relates also to a terpene biosynthetic gene cluster and regulatory regions of such terpene biosynthetic gene cluster usable is production of terpenes, use of regulator for regulating the terpene production and use of Aspergillus nidulans FGSC A4 for producing terpenes. The method of invention provides higher yields of enriched terpene product without essential amount of side-products.
摘要翻译： 本发明涉及一种在真菌中生产萜烯的方法，其中具有萜烯生物合成基因的萜烯生物合成基因簇和与所述基因可操作地连接的调节区被激活。 本发明还涉及萜烯生物合成基因簇和这种萜烯生物合成基因簇的调节区域，可用于生产萜烯，使用调节剂调节萜烯生产和使用构巢曲霉FGSC A4来生产萜烯。 本发明的方法提供了较高收率的富含萜烯产物，而没有必需量的副产物。
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