Manufacture of xylonic acid
    1.
    发明授权
    Manufacture of xylonic acid 有权
    木糖酸的制造

    公开(公告)号:US09315834B2

    公开(公告)日:2016-04-19

    申请号:US13256559

    申请日:2010-03-17

    CPC分类号: C12P7/58 C12N9/0006

    摘要: Provided is a method for producing xylonic acid from xylose with a recombinant fungal strain that is genetically modified to express a xylose dehydrogenase gene, which is able to convert xylose to xylonolactone, which is spontaneously or enzymatically hydrolyzed to xylonic acid. The xylonic acid is excreted outside the host cell. Xylonate production may be coupled with xylitol production. Alternatively, if xylitol production is not desired, its production is reduced by removing the aldose reductase (or specific xylose reductase) enzyme, which converts xylose to xylitol. Expression of a heterologous lactonase encoding gene may result in higher acid concentrations. The method is suitable for producing xylonic acid from a hemicellulose hydrolysate such as hydrolyzed lignocellulosic plant biomass.

    摘要翻译: 本发明提供了一种从木糖中生产木糖的方法,该重组真菌菌株被遗传修饰以表达木糖脱氢酶基因,其能够将木糖转化为己内酰胺,该木糖自发或酶促水解成木糖酸。 木糖酸在宿主细胞外排出。 木糖苷生产可能与木糖醇生产相结合。 或者,如果不需要木糖醇生产,则通过除去将木糖转化为木糖醇的醛糖还原酶(或特异性木糖还原酶)酶来降低其产生。 异源内含子编码基因的表达可能导致较高的酸浓度。 该方法适用于从半纤维素水解产物如水解木质纤维素植物生物质生产木糖酸。

    Method for producing terpenes
    2.
    发明申请
    Method for producing terpenes 有权
    生产萜烯的方法

    公开(公告)号:US20140045238A1

    公开(公告)日:2014-02-13

    申请号:US13883995

    申请日:2011-11-14

    IPC分类号: C12P5/00

    摘要: The present invention relates to a method for producing terpenes in fungi, wherein a terpene biosynthetic gene cluster having terpene biosynthetic genes and regulatory regions operably linked to said genes is activated. The invention relates also to a terpene biosynthetic gene cluster and regulatory regions of such terpene biosynthetic gene cluster usable is production of terpenes, use of regulator for regulating the terpene production and use of Aspergillus nidulans FGSC A4 for producing terpenes. The method of invention provides higher yields of enriched terpene product without essential amount of side-products.

    摘要翻译: 本发明涉及一种在真菌中生产萜烯的方法,其中具有萜烯生物合成基因的萜烯生物合成基因簇和与所述基因可操作地连接的调节区被激活。 本发明还涉及萜烯生物合成基因簇和这种萜烯生物合成基因簇的调节区域,可用于生产萜烯,使用调节剂调节萜烯生产和使用构巢曲霉FGSC A4来生产萜烯。 本发明的方法提供了较高收率的富含萜烯产物,而没有必需量的副产物。

    GENETICALLY MODIFIED FUNGI AND THEIR USE IN LIPID PRODUCTION
    3.
    发明申请
    GENETICALLY MODIFIED FUNGI AND THEIR USE IN LIPID PRODUCTION 有权
    遗传改良真菌及其在脂生产中的应用

    公开(公告)号:US20130102042A1

    公开(公告)日:2013-04-25

    申请号:US13806281

    申请日:2011-06-21

    IPC分类号: C12P7/64 C12N15/80

    摘要: The invention refers to fungal cells, and especially to oleaginous fungal cells that have been genetically modified to produce enzymes of the pyruvate dehydrogenase bypass route to enhance their lipid production. Especially the cells are modified to over-express genes encoding pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD) and/or acetyl-CoA synthetase (ACS), optionally together with a gene encoding diacylglycerol acyltransferase (DAT), or to express genes encoding PDC together with ALD and/or ACS. Methods of producing lipids, biofuels and lubricants using the modified fungi are also disclosed as well as expression cassettes useful therein. A new enzyme having phosholipid: diacylglycerol acyltransferase (PDAT)activity it and a polynucleotide encoding it are also disclosed, which are useful in the lipid production. A recombinant Cryptococcus cell and its construction is described.

    摘要翻译: 本发明涉及真菌细胞,尤其涉及已被遗传修饰以产生丙酮酸脱氢酶旁路途径的酶以增强其脂质产生的油质真菌细胞。 特别地,细胞被修饰为过度表达编码丙酮酸脱羧酶(PDC),乙醛脱氢酶(ALD)和/或乙酰辅酶A合成酶(ACS)的基因,任选与编码二酰基甘油酰基转移酶(DAT)的基因一起,或表达编码 PDC与ALD和/或ACS。 还公开了使用改性真菌生产脂质,生物燃料和润滑剂的方法以及在其中有用的表达盒。 还公开了具有磷脂的新型酶:二酰基甘油酰基转移酶(PDAT)活性及其编码它的多核苷酸,其可用于脂质生产。 描述了重组隐球藻细胞及其构建。

    Method for producing terpenes
    8.
    发明授权
    Method for producing terpenes 有权
    生产萜烯的方法

    公开(公告)号:US09238826B2

    公开(公告)日:2016-01-19

    申请号:US13883995

    申请日:2011-11-14

    摘要: The present invention relates to a method for producing terpenes in fungi, wherein a terpene biosynthetic gene cluster having terpene biosynthetic genes and regulatory regions operably linked to said genes is activated. The invention relates also to a terpene biosynthetic gene duster and regulatory regions of such terpene biosynthetic gene cluster usable is production of terpenes, use of regulator for regulating the terpene production and use of Aspergillus nidulans FGSC A4 for producing terpenes. The method of invention provides higher yields of enriched terpene product without essential amount of side-products.

    摘要翻译: 本发明涉及一种在真菌中生产萜烯的方法,其中具有萜烯生物合成基因的萜烯生物合成基因簇和与所述基因可操作地连接的调节区被激活。 本发明还涉及萜烯生物合成基因除尘器和这种萜烯生物合成基因簇的调节区域,可用于生产萜烯,使用调节剂来调节萜烯生产和使用构巢曲霉FGSC A4来生产萜烯。 本发明的方法提供了较高收率的富含萜烯产物,而没有必需量的副产物。