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公开(公告)号：US10144925B2
公开(公告)日：2018-12-04
申请号：US14651071
申请日：2012-12-13
Applicant: NANJING UNIVERSITY OF TECHNOLOGY
Inventor： Hanjie Ying , Yong Chen , Qingguo Liu , Jinglan Wu , Xiaochun Chen , Jingjing Xie
IPC: C12N11/08 , C12N11/12 , C12P7/06 , C12N11/02 , D06M13/123 , D06M13/332 , D06M13/418 , D06M15/59
Abstract: Provided is a preparation method of a yeast cell immobilization medium, which comprises the following steps: (1) boiling a fiber material in boiling water and drying the fiber material; (2) soaking the fiber material in a surface modified aqueous solution with a concentration of 1-100 g/L, using hydrochloric acid to adjust a PH of the solution to 7.0, fully rinsing the fiber material in deionized water and drying the fiber material; (3) soaking the fiber material in a cross-linking agent aqueous solution with a concentration of 1-100 g/L, fully rinsing the fiber material in deionized water and drying the fiber material; and (4) attaching the fiber material to supporting framework. Also provided is the yeast cell immobilization medium prepared using the preparation method and a method for producing ethanol using the yeast cell immobilization medium.
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22.发明申请Strong Secretory Signal Peptide Enhancing Small Peptide Motifs and the Use Thereof 审中-公开强分泌信号肽增强小肽主题及其用途公开(公告)号：US20160145303A1
公开(公告)日：2016-05-26
申请号：US14786160
申请日：2014-04-25
Applicant: NANJING UNIVERSITY OF TECHNOLOGY
Inventor： Bingfang HE , Wenhua CHEN , Lan MI , Shanshan WU , Yun ZHU , Shanshan CHEN
CPC classification number: C07K7/06 , C07K7/08 , C07K14/00 , C07K2319/02 , C12N9/2402 , C12N9/2454 , C12N15/625 , C12P21/02 , C12Y302/01 , C12Y302/01011
Abstract: The present invention belongs to protein engineering and genetic engineering fields, relating to a strong secretory signal peptide enhancing small peptide motifs and the use thereof. The strong secretory signal peptide enhancing small peptide motifs of the present invention have the amino acid sequence of the following formula: M (αXβYγ/αYβXγ)n, wherein X represents an acidic amino acid; Y represents an alkaline amino acid; α is 0 to 2 neutral amino acid(s); β represents 0 to 2 neutral amino acid(s); γ represents 1 to 10 neutral amino acid(s); n is 1 to 3. With regard to the use of the strong secretory signal peptide enhancing small peptide motifs of the present invention, it is a method for constructing a vector enhancing the secretion ability of common signal peptides to improve the secretory expression of exogenous proteins.
Abstract translation: 本发明属于蛋白质工程和遗传工程领域，涉及增强小肽基序的强分泌信号肽及其用途。 增强本发明小肽基序的强分泌信号肽具有下式的氨基酸序列：M（αX＆bgr;Yγ/αY＆bgr;Xγ）n，其中X表示酸性氨基酸; Y表示碱性氨基酸; α为0至2个中性氨基酸; ＆bgr 代表0至2个中性氨基酸; γ表示1〜10个中性氨基酸; n为1〜3。关于使用本发明的强分泌信号肽增强小肽基序，是构建增强共同信号肽分泌能力以增强外源蛋白质分泌表达的载体的方法 。
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23.发明授权Method for synthesizing 2,7-dimethyl-2,4,6-octatriene-1,8-dialdehyde 有权合成2,7-二甲基-2,4,6-辛二烯-1,8-二醛的方法公开(公告)号：US09187394B2
公开(公告)日：2015-11-17
申请号：US14369164
申请日：2012-12-10
Applicant: NANJING UNIVERSITY OF TECHNOLOGY , Zhejiang Medicine Co., Ltd. Xinchang Pharmaceutical Factory
Inventor： Chunlei Lv , Shiqing Pi , Jianhui Chen , Dingqiang Lu , Pingkai Ouyang
CPC classification number: C07C45/42 , C07C1/324 , C07C41/06 , C07C41/18 , C07C41/22 , C07C45/52 , C07F9/38 , C07C47/21
Abstract: Provided in the present invention is a method for synthesizing 2,7-dimethyl-2,4,6-octatriene-1,8-dialdehyde. The synthesis method comprises the following steps: (1) adding acetaldehyde diethyl acetal and ethyl-(1-propenyl)-ether under the effect of a catalyst to produce 1,1,3-triethoxy-2-methyl-butane; (2) pyrolysis synthesizing 1,1,3-triethoxy-2-methyl-butane under the catalytic effects of isoquinoline and p-Toluenesulfonic acid to produce 1-methoxy-2-methyl-1,3-butadiene; (3) dissolving 1-methoxy-2-methyl-1,3-butadiene in anhydrous ethanol solvent for synthesis with a phase transfer catalyst, cetyl-trimethyl ammonium bromide, and a chlorinating agent, trichloroisocyanuric acid, to generate 4,4-diethoxy-3-methyl-1-chloro-butene; (4) combining 4,4-diethoxy-3-methyl-1-chloro-butene with a triphenylphosphine salt to produce a phosphonium salt; and (5) condensing the phosphonium salt under the effects of hydrogen peroxide in conjunction with sodium carbonate solution to generate 1,1,8,8-tetramethyl-2,7-dimethyl-2,4,6-octatriene; then hydrolyzing under acidic conditions to synthesize 2,7-dimethyl-2,4,6-octatriene-1,8-dialdehyde. The present invention has a simple process route, is easy to operate, and has mild conditions, great yield, and great industrial value.
Abstract translation: 本发明提供了合成2,7-二甲基-2,4,6-辛二烯-1,8-二醛的方法。 合成方法包括以下步骤：（1）在催化剂作用下加入乙醛缩二乙醇和乙基 - （1-丙烯基） - 醚，制得1,1,3-三乙氧基-2-甲基 - 丁烷; （2）在异喹啉和对甲苯磺酸的催化作用下热解合成1,1,3-三乙氧基-2-甲基丁烷，得到1-甲氧基-2-甲基-1,3-丁二烯; （3）将1-甲氧基-2-甲基-1,3-丁二烯溶于无水乙醇溶剂中，用相转移催化剂，十六烷基三甲基溴化铵和氯化剂三氯异氰脲酸合成，得到4,4-二乙氧基 -3-甲基-1-氯 - 丁烯; （4）将4,4-二乙氧基-3-甲基-1-氯 - 丁烯与三苯基膦盐结合以产生鏻盐; 和（5）在过氧化氢与碳酸钠溶液的作用下使鏻盐缩合，生成1,1,8,8-四甲基-2,7-二甲基-2,4,6-辛二烯; 然后在酸性条件下水解合成2,7-二甲基-2,4,6-辛二烯-1,8-二醛。 本发明工艺路线简单，操作方便，条件温和，产量大，工业价值高。
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24.发明申请PREPARATION METHOD OF 3,7,11- TRIMETHYLDODEC -2,4,6,10-TETRAENE-1-YL-PHOSPHONIC SALT 有权3,7,11-三甲基环己酮-2,4,6,10-四烯-1-基-L-磷酸盐盐的制备方法公开(公告)号：US20140378709A1
公开(公告)日：2014-12-25
申请号：US14369102
申请日：2012-12-10
Applicant: NANJING UNIVERSITY OF TECHNOLOGY , Zhejiang Medicine Co., Ltd. Xinchang Pharmaceutical Factory
Inventor： Chunlei Lv , Shiqing Pi , Jianhui Chen , Dingqiang Lu , Pingkai Ouyang
IPC: C07F9/54
CPC classification number: C07F9/5442 , C07F9/5428
Abstract: Provided in the present invention is a preparation method for a phosphonic salt, comprising the step of: reacting 3,7,11-trimethyldodec-1,4,6,10-tetraene-3-ol with triarylphosphine and an acid in an alcohol solvent at 50-100° C. to form the phosphonic salt, wherein the acid is a sulfamic acid or methanesulfonic acid, and the alcohol solvent is a straight chain monohydric alcohol containing 1-5 carbon atoms. The method is performed in nearly neutral conditions, greatly reducing the generation of impurities and greatly obtaining phosphonic salt with an increased E content. The yield of lycopene obtained by using the phosphonic salt as a raw material is high.
Abstract translation: 本发明提供了一种膦酸盐的制备方法，其包括以下步骤：使3,7,11-三甲基十二烷-1,4,6,10-四烯-3-醇与三芳基膦和酸在醇溶剂中反应 在50-100℃下形成膦酸盐，其中酸是氨基磺酸或甲磺酸，醇溶剂是含有1-5个碳原子的直链一元醇。 该方法在接近中性条件下进行，大大减少杂质的产生，并大大地获得具有增加的E含量的膦酸盐。 通过使用膦酸盐作为原料获得的番茄红素的产率高。
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公开(公告)号：US11248244B2
公开(公告)日：2022-02-15
申请号：US16607861
申请日：2017-05-10
Applicant: Nanjing University of Technology
Inventor： He Huang , Lujing Ren , Shenglan Chen , Lingjun Geng , Xiaojun Ji , Xuechao Hu
Abstract: Provided is a Schizochytrium limacinum strain, a building method therefor and an application thereof. The strain disclosed is classified and named as Schizochytrium sp. HX-RS, and the preservation number is CCTCC NO: M2017046. An acyltransferase functional domain originating from Shewanella PKS enzyme is adopted instead of an acyltransferase functional domain originating from Schizochytrium sp. PKS enzyme, and the strain is obtained by performing flat panel screening and acclimation screening with a high rotation seed and a low temperature.
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Patent Agency Ranking
公开(公告)号：US20210098731A1
公开(公告)日：2021-04-01
申请号：US16771230
申请日：2018-12-05
Applicant: NANJING UNIVERSITY OF TECHNOLOGY
Inventor： Jianpu WANG , Nana WANG , Yu CAO , Wei HUANG
Abstract: The disclosure discloses a perovskite film layer, a device and a preparation method for effectively improving the efficiency of perovskite optoelectronics. The perovskite film layer consists of a layer with discontinuous, irregularly distributed perovskite crystal grains and an organic insulating layer with a low refractive index embedded between the perovskite crystal grains. The perovskite crystal grains form a plurality of convex portions, and the organic insulating layer forms a plurality of concave portions between the convex portions. By adding an excess of an alkylammonium salt and/or an organic molecule with a specific functional group to perovskite precursor solution, a concave-convex film layer structure is spontaneously formed, and an upper charge transport layer and an electrode form pleated concave-convex structures. Such a special perovskite thin film structure formed by a simple solution method can effectively improve the light-outcoupling efficiency and enhance the performance of the perovskite light-emitting device.
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27.发明申请公开(公告)号：US20190262779A1
公开(公告)日：2019-08-29
申请号：US16348819
申请日：2017-06-09
Applicant: NANJING UNIVERSITY OF TECHNOLOGY
Inventor： Xuehong GU , Xun YAO , Li PENG
Abstract: The invention relates to a method for preparing a hierarchical porous zeolite membrane and an application thereof, comprising the following steps: a mesoporous structure-directing agent is added to limit the growth of zeolite crystals, and self-assembled in the crystallization process to generate a mesoporous structure. Based on a seed crystal induced secondary nucleation mechanism, this method can realize one-step hydrothermal synthesis of hierarchical porous zeolite membrane with the advantages of mild and controllable synthesis conditions, simple process, good repeatability, reduced energy consumption and cost savings. The hierarchical porous zeolite membrane prepared by the method has good cut-off performance, and the cut-off molecular weight is adjustable between 200 to 500,000 Da.
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公开(公告)号：US20190040003A1
公开(公告)日：2019-02-07
申请号：US16062630
申请日：2015-07-31
Applicant: Nanjing University of Technology
Inventor： Weihong XING , Honglin MAO , Rizhi CHEN , Hongnian SHANG , Nanping XU
IPC: C07C249/04 , C07C251/44 , C07C251/38
Abstract: The invention relates to a solvent-free green ammoximation process based on membrane distribution with a procedure as: adding TS-1 catalyst and ketone into a reactor in advance; setting the stirring speed and reaction temperature; after reaching the set temperature, adding a certain amount of ammonia and hydrogen peroxide into a reaction solution, wherein the hydrogen peroxide is fed in a way of using membrane as a distributor, the ammonia is fed in a continuous or semi-continuous manner; oxime is produced upon the reaction. The advantages of the invention include the mild reaction conditions, high reacting efficiency, simple operation and environmentally-friendly process. And there is no need to add any solvent during the reaction process. During the ammoximation reaction, both the conversion rate of the ketone and the selectivity of the oxime can be over 98.0%.
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公开(公告)号：US09765098B2
公开(公告)日：2017-09-19
申请号：US14369102
申请日：2012-12-10
Applicant: NANJING UNIVERSITY OF TECHNOLOGY , Zhejiang Medicine Co., Ltd. Xinchang Pharmaceutical Factory
Inventor： Chunlei Lv , Shiqing Pi , Jianhui Chen , Dingqiang Lu , Pingkai Ouyang
CPC classification number: C07F9/5442 , C07F9/5428
Abstract: Provided in the present invention is a preparation method for a phosphonic salt, comprising the step of: reacting 3,7,11-trimethyldodec-1,4,6,10-tetraene-3-ol with triarylphosphine and an acid in an alcohol solvent at 50-100° C. to form the phosphonic salt, wherein the acid is a sulfamic acid or methanesulfonic acid, and the alcohol solvent is a straight chain monohydric alcohol containing 1-5 carbon atoms. The method is performed in nearly neutral conditions, greatly reducing the generation of impurities and greatly obtaining phosphonic salt with an increased E content. The yield of lycopene obtained by using the phosphonic salt as a raw material is high.
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30.发明申请公开(公告)号：US20170044021A1
公开(公告)日：2017-02-16
申请号：US15306491
申请日：2014-09-12
Applicant: Nanjing University of Technology
Inventor： Weihong XING , Zhilong XU , Nanping XU , Zhaoxiang ZHONG , Feng ZHANG
CPC classification number: C01G9/02 , B01J14/00 , B01J19/2415 , B01J19/2475 , B01J2219/00033 , B82Y30/00 , C01P2002/72 , C01P2004/03
Abstract: The present invention relaters to a method for continuously preparing a nano zinc oxide with a membrane reactor. A zinc salt solution and a precipitator solution required for the preparation of a zinc oxide are respectively used as dispersion phases, and under the action of a certain pressure, the two reaction solutions respectively penetrate through a membrane tube at a certain rate and disperse quickly under the action of a shear force and react, producing a precursor precipitate. A precursor suspension penetrates through the membrane tube continuously and circularly after being pressurized by a pump, and at the same time, deionized water as a washing fluid is added to a suspension storage tank, wherein impurity ions penetrate through membrane pores and are discharged along with the liquid medium; after the concentration of the impurity ions meets requirements, the concentrated solution is discharged continuously and then spray-dried to obtain a basic zinc carbonate precursor powder. The basic zinc carbonate powder is calcined under certain conditions to obtain the nanostructured zinc oxide powder. The continuous preparation and washing of a powder can be achieved by coupling a membrane washing technique with a membrane dispersion technique. The procedure is simple, the structure of zinc oxide is easy to control and the yield is high.
Abstract translation: 本发明涉及一种用膜反应器连续制备纳米氧化锌的方法。 分别使用锌盐溶液和制备氧化锌所需的沉淀剂溶液作为分散相，并且在一定压力的作用下，两种反应溶液分别以一定速率穿过膜管并快速分散在 剪切力的作用并反应，产生前体沉淀。 前驱体悬浮液在被泵加压之后，连续循环地穿过膜管，同时将去离子水作为洗涤液加入到悬浮储罐中，其中杂质离子穿过膜孔并与 液体介质; 杂质离子浓度满足要求后，浓缩溶液连续排出，然后喷雾干燥，得到碱式碳酸锌前体粉末。 碱性碳酸锌粉末在一定条件下煅烧得到纳米结构氧化锌粉末。 通过将膜洗涤技术与膜分散技术结合可以实现粉末的连续制备和洗涤。 操作简单，氧化锌结构易于控制，产量高。
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