公开(公告)号：US10526387B2

公开(公告)日：2020-01-07

申请号：US15341528

申请日：2016-11-02

Applicant: BioNTech AG

Inventor： Thorsten Klamp ,  Ugur Sahin ,  Ozlem Tureci ,  Michael Koslowski ,  Thomas Hiller ,  Jens Schumacher

IPC: C07K14/47 ,  A61K39/00 ,  C07K14/005 ,  C12N7/00

Abstract: The present invention relates to tumor immunotherapy, in particular to tumor vaccination, using chimeric proteins comprising all or a portion of a hepatitis B virus core antigen protein and an amino acid sequence comprising an epitope derived from the extracellular portion of a tumor-associated antigen. In particular, the present invention provides virus-like particles comprising said chimeric proteins, which are useful for eliciting a humoral immune response in a subject against the tumor-associated antigen, in particular against cells carrying said tumor-associated antigen on their surface, wherein the tumor-associated antigen is a self-protein in said subject.

公开(公告)号：US20190062762A1

公开(公告)日：2019-02-28

申请号：US16143520

申请日：2018-09-27

Applicant: BioNTech AG

Inventor： Ugur Sahin ,  Silke Holtkamp ,  Ozlem Tureci ,  Sebastian Kreiter

IPC: C12N15/68 ,  C12N15/85 ,  C12N15/67

Abstract: It was the object of the present invention to provide RNA with increased stability and translation efficiency and means for obtaining such RNA. It should be possible to obtain increased grades of expression by using said RNA in gene therapy approaches.

公开(公告)号：US20180177885A1

公开(公告)日：2018-06-28

申请号：US15895402

申请日：2018-02-13

Applicant: BioNTech AG

Inventor： Ozlem Tureci ,  Ugur Sahin ,  Sebastian Kreiter

IPC: A61K47/64 ,  C07K14/74 ,  C07K14/47 ,  A61K48/00

CPC classification number: A61K47/64 ,  A61K48/00 ,  C07K14/4748 ,  C07K14/70539 ,  C07K2319/02 ,  C07K2319/03

Abstract: The present invention relates to fusion molecules of antigens, the nucleic acids coding therefor and the use of such fusion molecules and nucleic acids. In particular, said invention relates to fusion molecules, comprising an antigen and the trans-membrane region and cytoplasmic region of a MHC molecule and/or the cytoplasmic region of a MHC or a SNARE molecule.

公开(公告)号：US20170009244A1

公开(公告)日：2017-01-12

申请号：US15217555

申请日：2016-07-22

Applicant: BioNTech AG

Inventor： Ugur Sahin ,  Silke Holtkamp ,  Ozlem Tureci ,  Sebastian Kreiter

IPC: C12N15/68 ,  C12N15/85

CPC classification number: C12N15/68 ,  C12N15/67 ,  C12N15/85

Abstract: It was the object of the present invention to provide RNA with increased stability and translation efficiency and means for obtaining such RNA. It should be possible to obtain increased grades of expression by using said RNA in gene therapy approaches.

Abstract translation: 本发明的目的是提供具有增加的稳定性和翻译效率的RNA以及获得这种RNA的手段。 通过在基因治疗方法中使用所述RNA可以获得更高等级的表达。

公开(公告)号：US20160215351A1

公开(公告)日：2016-07-28

申请号：US15003530

申请日：2016-01-21

Applicant: BioNTech AG

Inventor： Ugur Sahin ,  Ozlem Tureci ,  Michael Koslowski

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C07K14/4748 ,  C07K16/30 ,  C12Q2600/118 ,  C12Q2600/158

Abstract: The present technology relates to genetic products the expression of which is associated with cancer diseases. The present technology also relates to the therapy and diagnosis of diseases in which the genetic products are expressed or aberrantly expressed, in particular cancer diseases.

Abstract translation: 本技术涉及与癌症疾病相关的遗传产物。 本技术还涉及遗传产物表达或异常表达，特别是癌症疾病的疾病的治疗和诊断。

公开(公告)号：US09139639B2

公开(公告)日：2015-09-22

申请号：US14571425

申请日：2014-12-16

Applicant: BioNTech AG ,  Universitatsmedizin der Johannes Gutenberg-Universitat Mainz

Inventor： Ugur Sahin ,  Ozlem Tureci ,  Torsten Seppmann ,  Holger Hoff ,  Jens Schumacher

IPC: C12N15/12 ,  C12N15/11 ,  C12N15/63 ,  C07K14/705 ,  C07K16/00 ,  C12N5/0781

CPC classification number: C07K14/70578 ,  C07K16/00 ,  C07K2319/30 ,  C12N5/0635

Abstract: The present invention provides recombinant proteins comprising the amino acid sequence of an intracellular segment of CD40 and an amino acid sequence mediating the association of the recombinant protein with the constant region of an immunoglobulin heavy chain. The recombinant proteins according to the present invention are—useful for inducing clonal expansion of a B cell having a predetermined antigen-specificity without the need for T cell or CD40L mediated co-stimulation. Thus, the present invention provides tools for clonal expansion of B cells specific for an antigen of interest and the production of B cells secreting antibodies specific for an antigen of interest. The recombinant proteins of the present invention may also be used for generating fully human monoclonal antibodies with a predetermined antigen-specificity from the B cell repertoire of a human subject.

Abstract translation: 本发明提供重组蛋白，其包含CD40的细胞内节段的氨基酸序列和介导重组蛋白与免疫球蛋白重链的恒定区的缔合的氨基酸序列。 根据本发明的重组蛋白质可用于诱导具有预定抗原特异性的B细胞的克隆扩增，而不需要T细胞或CD40L介导的共刺激。 因此，本发明提供了用于针对感兴趣的抗原特异性的B细胞的克隆扩增的工具，以及产生分泌针对感兴趣的抗原的抗体的B细胞。 本发明的重组蛋白还可用于从人受试者的B细胞库中产生具有预定抗原特异性的完全人单克隆抗体。