公开(公告)号：US20200009263A1

公开(公告)日：2020-01-09

申请号：US16062831

申请日：2016-12-16

Applicant: Eisai R&D Management Co., Ltd.

Inventor： Jared Spidel ,  Earl Albone

IPC: A61K47/68 ,  C07K16/00 ,  A61K47/60

Abstract: Provided herein are conjugated immunoglobulins and methods for generating conjugated immunoglobulins using a microbial transglutaminase.

公开(公告)号：US20190202933A1

公开(公告)日：2019-07-04

申请号：US16353326

申请日：2019-03-14

Applicant: Eisai R&D Management Co., Ltd.

Inventor： Luigi Grasso ,  Jared Spidel ,  James Bradford Kline ,  Earl Albone

IPC: C07K16/40 ,  A61K47/68 ,  C07K16/28 ,  G01N33/533 ,  C07K16/30

CPC classification number: C07K16/40 ,  A61K47/6849 ,  A61K47/6851 ,  A61K47/6898 ,  A61K2039/507 ,  C07K16/28 ,  C07K16/2851 ,  C07K16/30 ,  C07K2317/20 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/73 ,  C07K2317/92 ,  G01N33/533

Abstract: Provided herein are methods for generating conjugated immunoglobulins, the method comprising: decapping a cysteine at amino acid position 80 (“Cys80”) in a light chain variable region of an immunoglobulin, wherein the immunoglobulin comprises a heavy chain variable region and the light chain variable region; and conjugating a thiol-reactive compound to the Cys80, wherein the thiol-reactive compound comprises a thiol-reactive group. Antigen-binding molecules and methods for generating the same, immunoglobulins as well as nucleic acid molecules encoding the immunoglobulins and host cells comprising the nucleic acid molecules, conjugated immunoglobulins, and light chain variable regions for use in a conjugated immunoglobulin are also provided.

公开(公告)号：US10273310B2

公开(公告)日：2019-04-30

申请号：US15185879

申请日：2016-06-17

Applicant: Eisai R&D Management Co., Ltd.

Inventor： Luigi Grasso ,  Jared Spidel ,  James Bradford Kline ,  Earl Albone

IPC: C07K16/40 ,  A61K47/68 ,  A61K39/00 ,  C07K16/28 ,  C07K16/30 ,  G01N33/533

Abstract: Provided herein are methods for generating conjugated immunoglobulins, the method comprising: decapping a cysteine at amino acid position 80 (“Cys80”) in a light chain variable region of an immunoglobulin, wherein the immunoglobulin comprises a heavy chain variable region and the light chain variable region; and conjugating a thiol-reactive compound to the Cys80, wherein the thiol-reactive compound comprises a thiol-reactive group. Antigen-binding molecules and methods for generating the same, immunoglobulins as well as nucleic acid molecules encoding the immunoglobulins and host cells comprising the nucleic acid molecules, conjugated immunoglobulins, and light chain variable regions for use in a conjugated immunoglobulin are also provided.