公开(公告)号：US20190136225A1

公开(公告)日：2019-05-09

申请号：US16094070

申请日：2017-04-26

Applicant: Gen-Probe Incorporated

Inventor： Kui Gao ,  Jijumon Chelliserry ,  Jeffrey Linnen

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/70

Abstract: Disclosed herein are lysis reagents for lysing red blood cells, thereby releasing an analyte, such as RNA from a host or pathogen, in a form suitable for analysis. The reagent includes at least a buffer, a detergent and one or both of a chloride containing salt and an anti-coagulant. The reagent serves to lyse blood cells, protect the released analyte from degradation in the lysate, and is compatible with subsequent steps for analysis of the analyte such as target capture, amplification, detection, or sequencing.

公开(公告)号：US20180334734A1

公开(公告)日：2018-11-22

申请号：US16015539

申请日：2018-06-22

Applicant: Gen-Probe Incorporated

Inventor： Kui Gao ,  Edgar O. Ong ,  Jennifer Cole ,  Jeffrey M. Linnen

IPC: C12Q1/70

CPC classification number: C12Q1/707

Abstract: Disclosed are nucleic acid oligomers, including amplification oligomers, capture probes, and detection probes, for detection of Hepatitis E Virus (HEV) nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits.

公开(公告)号：US10093989B2

公开(公告)日：2018-10-09

申请号：US14918131

申请日：2015-10-20

Applicant: Gen-Probe Incorporated

Inventor： Jijumon Chelliserry ,  Kui Gao ,  Jeffrey M. Linnen

IPC: C12Q1/68 ,  C12Q1/6893 ,  C12N1/06 ,  G01N33/48

Abstract: The invention provides a lysis reagent for lysing red blood cells, thereby releasing a target, such as RNA from a parasitic organism, in a form suitable for analysis. The reagent includes at least ammonium chloride and an anionic detergent, and may include an anti-coagulant. The reagent serves to lyse red blood cells, protect the released target from degradation in the lysate, and is compatible with subsequent steps for analysis of the target such as target capture, amplification, detection, or sequencing.

公开(公告)号：US10053742B2

公开(公告)日：2018-08-21

申请号：US14911667

申请日：2014-08-14

Applicant: GEN-PROBE INCORPORATED

Inventor： Kui Gao ,  Edgar O. Ong ,  Jennifer Cole ,  Jeffrey M. Linnen

IPC: C07H21/04 ,  C12Q1/70

CPC classification number: C12Q1/707

Abstract: Disclosed are nucleic acid oligomers, including amplification oligomers, capture probes, and detection probes, for detection of Hepatitis E Virus (HEV) nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits.