公开(公告)号：US11572596B2

公开(公告)日：2023-02-07

申请号：US17012946

申请日：2020-09-04

Applicant: Gen-Probe Incorporated

Inventor： Kui Gao ,  Edgar O. Ong ,  Jennifer Cole ,  Jeffrey M. Linnen

IPC: C12Q1/70

Abstract: Disclosed are nucleic acid oligomers, including amplification oligomers, capture probes, and detection probes, for detection of Hepatitis E Virus (HEV) nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits.

公开(公告)号：US11162145B2

公开(公告)日：2021-11-02

申请号：US16869720

申请日：2020-05-08

Applicant: Gen-Probe Incorporated

Inventor： Jijumon Chelliserry ,  Kui Gao ,  Jeffrey M. Linnen

IPC: C12Q1/6893 ,  C12N1/06 ,  G01N33/48 ,  C12Q1/6806

Abstract: The invention provides a lysis reagent for lysing red blood cells, thereby releasing a target, such as RNA from a parasitic organism, in a form suitable for analysis. The reagent includes at least ammonium chloride and an anionic detergent, and may include an anti-coagulant. The reagent serves to lyse red blood cells, protect the released target from degradation in the lysate, and is compatible with subsequent steps for analysis of the target such as target capture, amplification, detection, or sequencing.

公开(公告)号：US10508312B2

公开(公告)日：2019-12-17

申请号：US15618834

申请日：2017-06-09

Applicant: GEN-PROBE INCORPORATED

Inventor： Kui Gao ,  Jeffrey M. Linnen

IPC: C12Q1/70

Abstract: Disclosed are nucleic acid oligomers, including amplification oligomers, capture probes, and detection probes, for detection of Zika virus nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits.

公开(公告)号：US12071615B2

公开(公告)日：2024-08-27

申请号：US17485599

申请日：2021-09-27

Applicant: Gen-Probe Incorporated

Inventor： Kui Gao ,  Jijumon Chelliserry ,  Jeffrey Linnen

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/70

CPC classification number: C12N15/1003 ,  C12Q1/6806 ,  C12Q1/70 ,  Y02A50/30 ,  C12Q1/6806 ,  C12Q2525/161 ,  C12Q2527/125 ,  C12Q2531/143 ,  C12Q2565/501 ,  C12Q1/70 ,  C12Q2525/161 ,  C12Q2527/125 ,  C12Q2531/143 ,  C12Q2565/501

Abstract: Disclosed herein are lysis reagents for lysing red blood cells, thereby releasing an analyte, such as RNA from a host or pathogen, in a form suitable for analysis. The reagent includes at least a buffer, a detergent and one or both of a chloride containing salt and an anti-coagulant. The reagent serves to lyse blood cells, protect the released analyte from degradation in the lysate, and is compatible with subsequent steps for analysis of the analyte such as target capture, amplification, detection, or sequencing.

公开(公告)号：US11821046B2

公开(公告)日：2023-11-21

申请号：US17032310

申请日：2020-09-25

Applicant: GEN-PROBE INCORPORATED

Inventor： Kui Gao ,  Jeffrey M. Linnen

IPC: C12Q1/70

CPC classification number: C12Q1/701 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: Disclosed are nucleic acid oligomers, including amplification oligomers, capture probes, and detection probes, for detection of Zika virus nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits.

公开(公告)号：US11015185B1

公开(公告)日：2021-05-25

申请号：US17134604

申请日：2020-12-28

Applicant: Gen-Probe Incorporated

Inventor： Kui Gao ,  Jijumon Chelliserry ,  Jeffrey Linnen

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/70

Abstract: Disclosed herein are lysis reagents for lysing red blood cells, thereby releasing an analyte, such as RNA from a host or pathogen, in a form suitable for analysis. The reagent includes at least a buffer, a detergent and one or both of a chloride containing salt and an anti-coagulant. The reagent serves to lyse blood cells, protect the released analyte from degradation in the lysate, and is compatible with subsequent steps for analysis of the analyte such as target capture, amplification, detection, or sequencing.

公开(公告)号：US10689713B2

公开(公告)日：2020-06-23

申请号：US16019829

申请日：2018-06-27

Applicant: Gen-Probe Incorporated

Inventor： Jijumon Chelliserry ,  Kui Gao ,  Jeffrey M. Linnen

IPC: C12Q1/68 ,  C12Q1/6893 ,  C12N1/06 ,  G01N33/48 ,  C12Q1/6806

Abstract: The invention provides a lysis reagent for lysing red blood cells, thereby releasing a target, such as RNA from a parasitic organism, in a form suitable for analysis. The reagent includes at least ammonium chloride and an anionic detergent, and may include an anti-coagulant. The reagent serves to lyse red blood cells, protect the released target from degradation in the lysate, and is compatible with subsequent steps for analysis of the target such as target capture, amplification, detection, or sequencing.

公开(公告)号：US11332798B2

公开(公告)日：2022-05-17

申请号：US16659375

申请日：2019-10-21

Applicant: GEN-PROBE INCORPORATED

Inventor： Kui Gao ,  Jeffrey M. Linnen

IPC: C12Q1/70

Abstract: Disclosed are nucleic acid oligomers, including amplification oligomers, capture probes, and detection probes, for detection of Zika virus nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits.

公开(公告)号：US20210139886A1

公开(公告)日：2021-05-13

申请号：US17134604

申请日：2020-12-28

Applicant: Gen-Probe Incorporated

Inventor： Kui Gao ,  Jijumon Chelliserry ,  Jeffrey Linnen

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/70

Abstract: Disclosed herein are lysis reagents for lysing red blood cells, thereby releasing an analyte, such as RNA from a host or pathogen, in a form suitable for analysis. The reagent includes at least a buffer, a detergent and one or both of a chloride containing salt and an anti-coagulant. The reagent serves to lyse blood cells, protect the released analyte from degradation in the lysate, and is compatible with subsequent steps for analysis of the analyte such as target capture, amplification, detection, or sequencing.

公开(公告)号：US10815541B2

公开(公告)日：2020-10-27

申请号：US16015539

申请日：2018-06-22

Applicant: Gen-Probe Incorporated

Inventor： Kui Gao ,  Edgar O. Ong ,  Jennifer Cole ,  Jeffrey M. Linnen

IPC: C12Q1/00 ,  C12Q1/70

Abstract: Disclosed are nucleic acid oligomers, including amplification oligomers, capture probes, and detection probes, for detection of Hepatitis E Virus (HEV) nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits.