公开(公告)号：US06261773B1

公开(公告)日：2001-07-17

申请号：US09268710

申请日：1999-03-16

申请人： Masaya Segawa ,  Motohiro Kondo ,  Yutaka Takarada

发明人： Masaya Segawa ,  Motohiro Kondo ,  Yutaka Takarada

IPC分类号： C12Q168

CPC分类号： C12Q1/6848 ,  C12Q2527/125

摘要： The present invention provide a process for sequence-specific nucleic acid amplification capable of improving the detection sensitivity and increasing the signal. In particular, the present invention provides a reagent for nucleic acid amplification containing at least one member selected from the group consisting of EDTA, NTA, UDA, CyDTA, DTPA, GEDTA, TTHA and their salts, specifically a reagent for nucleic acid amplification comprising, in addition to the at least one compound, a forward primer having a DNA sequence homologous to a sequence of a target RNA; a reverse primer having a DNA sequence complementary to a sequence of the target RNA and having a promoter for RNA polymerase attached to its 5′ end; ribonucleotides; deoxyribonucleotides; a reverse transcriptase or RNA-directed DNA polymerase; a RNase H; a DNA polymerase or a reverse transcriptase having DNA-directed DNA polymerase activity; and a RNA polymerase. The present invention also provides a process for nucleic acid amplification characterized by carrying out a nucleic acid amplification reaction in the presence of at least one member selected from the group consisting of EDTA, NTA, UDA, CyDTA, DTPA, GEDTA, TTHA and their salts.

摘要翻译： 本发明提供能够提高检测灵敏度和增加信号的序列特异性核酸扩增方法。 特别地，本发明提供了一种用于核酸扩增的试剂，其包含选自EDTA，NTA，UDA，CyDTA，DTPA，GEDTA，TTHA及其盐中的至少一种，特别是用于核酸扩增的试剂， 除了至少一种化合物之外，具有与靶RNA的序列同源的DNA序列的正向引物; 具有与靶RNA序列互补的DNA序列并具有连接于其5'末端的RNA聚合酶的启动子的反向引物; 核糖核苷酸 脱氧核糖核苷酸; 逆转录酶或RNA指导的DNA聚合酶; 核糖核酸酶H; 具有DNA定向DNA聚合酶活性的DNA聚合酶或逆转录酶; 和RNA聚合酶。 本发明还提供了一种核酸扩增方法，其特征在于在选自EDTA，NTA，UDA，CyDTA，DTPA，GEDTA，TTHA及其盐中的至少一种的存在下进行核酸扩增反应 。

公开(公告)号：US08708089B2

公开(公告)日：2014-04-29

申请号：US13054382

申请日：2009-07-15

申请人： Kouji Kitahata ,  Shiro Nakano ,  Masaya Segawa ,  Yoshiharu Nogami

发明人： Kouji Kitahata ,  Shiro Nakano ,  Masaya Segawa ,  Yoshiharu Nogami

IPC分类号： B62D5/04 ,  B62D5/00

CPC分类号： B62D5/0409 ,  B62D5/00 ,  B62D5/04 ,  F16H1/22 ,  F16H1/227 ,  F16H7/02

摘要： A vehicle steering apparatus includes an actuator that generates a steering force, and a first speed reduction mechanism connected to the actuator. To the first speed reduction mechanism, a second speed reduction mechanism is connected. To the second speed reduction mechanism, a steering mechanism is connected. A sub-assembly including the actuator and the first speed reduction mechanism is configured.

摘要翻译： 车辆转向装置包括产生转向力的致动器和连接到致动器的第一减速机构。 对于第一减速机构，连接有第二减速机构。 对于第二减速机构，连接有转向机构。 配置包括致动器和第一减速机构的子组件。

公开(公告)号：US20110120797A1

公开(公告)日：2011-05-26

申请号：US13054382

申请日：2009-07-15

申请人： Kouji Kitahata ,  Shiro Nakano ,  Masaya Segawa ,  Yoshiharu Nogami

发明人： Kouji Kitahata ,  Shiro Nakano ,  Masaya Segawa ,  Yoshiharu Nogami

IPC分类号： B62D5/04

CPC分类号： B62D5/0409 ,  B62D5/00 ,  B62D5/04 ,  F16H1/22 ,  F16H1/227 ,  F16H7/02

摘要： A vehicle steering apparatus includes an actuator that generates a steering force, and a first speed reduction mechanism connected to the actuator. To the first speed reduction mechanism, a second speed reduction mechanism is connected. To the second speed reduction mechanism, a steering mechanism is connected. A sub-assembly including the actuator and the first speed reduction mechanism is configured.

摘要翻译： 车辆转向装置包括产生转向力的致动器和连接到致动器的第一减速机构。 对于第一减速机构，连接有第二减速机构。 对于第二减速机构，连接有转向机构。 配置包括致动器和第一减速机构的子组件。

公开(公告)号：US20100203594A1

公开(公告)日：2010-08-12

申请号：US11596929

申请日：2005-05-12

申请人： Masaya Segawa ,  Takashi Nakajima ,  Masanori Oka

发明人： Masaya Segawa ,  Takashi Nakajima ,  Masanori Oka

IPC分类号： C12P19/34 ,  C12N9/10

CPC分类号： C12Q1/686 ,  C12N9/1252 ,  C12Q2527/113

摘要： [Problems] To achieve a high-speed PCR which can be completed in a period of several minutes and thus can be used for a new application.[Means for Solving Problems] A method for practicing a high-speed PCR under a temperature change of 10° C./sec or more, characterized in that use is made of a heat-resistant DNA polymerase exhibiting a speed of synthesizing a deoxyribonucleic acid of 100 base/sec or higher, and a reagent kit for a high-speed PCR, or use in the method.

摘要翻译： [问题]实现可以在几分钟内完成的高速PCR，因此可以用于新的应用。 解决问题的手段在10℃/秒以上的温度变化下进行高速PCR的方法，其特征在于，使用具有合成脱氧核糖核酸速度的耐热DNA聚合酶 100碱基/秒以上，以及用于高速PCR的试剂盒，或者在该方法中使用。

公开(公告)号：US07147080B2

公开(公告)日：2006-12-12

申请号：US10403094

申请日：2003-04-01

申请人： Masayasu Higashi ,  Shingo Maeda ,  Masaya Segawa ,  Shuuji Kimura ,  Ryouhei Hayama ,  Tomoyasu Kada

发明人： Masayasu Higashi ,  Shingo Maeda ,  Masaya Segawa ,  Shuuji Kimura ,  Ryouhei Hayama ,  Tomoyasu Kada

IPC分类号： B60K26/00

CPC分类号： B62D1/00 ,  B62D1/02 ,  B62D1/12 ,  Y10T74/20396

摘要： The steering device is constructed by supporting a center portion of a rectangle-plate-shaped operation plate at one end of a supporting shaft projected from the front face of a dashboard so as to freely swing in right and left directions. A steering apparatus for a vehicle having the steering device detects the swing direction and the swing angle of the operation plate with a steering angle sensor provided at a support portion and moves a steering mechanism in the detected direction in accordance with the detected angle so as to steer the vehicle. With this structure, a load in steering can be decreased and tiredness in driving can be reduced. Furthermore, such a steering apparatus can realize a steering device which physically handicapped drivers can operate easily.

摘要翻译： 转向装置通过在从仪表板的前表面突出的支撑轴的一端支撑矩形板状操作板的中心部分而构成，以便在左右方向上自由摆动。 具有转向装置的车辆用转向装置利用设置在支撑部的转向角传感器来检测操作板的摆动方向和摆动角度，并根据检测角度使检测方向上的转向机构移动， 引导车辆。 利用这种结构，可以减少转向中的负载并且可以减少驾驶中的疲劳。 此外，这种转向装置可以实现能够容易操作的驾驶员身体障碍的转向装置。

公开(公告)号：US06698542B2

公开(公告)日：2004-03-02

申请号：US10075212

申请日：2002-02-13

申请人： Katsutoshi Nishizaki ,  Shirou Nakano ,  Takanobu Takamatsu ,  Masaya Segawa ,  Ryouhei Hayama ,  Kazuhiro Kato

发明人： Katsutoshi Nishizaki ,  Shirou Nakano ,  Takanobu Takamatsu ,  Masaya Segawa ,  Ryouhei Hayama ,  Kazuhiro Kato

IPC分类号： B62D500

CPC分类号： B62D6/00 ,  B60T8/1755 ,  B60T8/328 ,  B60T2260/02 ,  B62D5/06 ,  B62D5/07

摘要： The steering device for vehicle changes the steering angle by transmitting the movements of the steering electrical actuator to the vehicle wheels. When the vehicle is steered in the stationary state, the hydraulic fluid can be supplied to the steering assistance force generating hydraulic actuator from the pump within the hydraulic pressure imparting unit. When the vehicle is not steered in the stationary state, the hydraulic fluid can be supplied to the brake device from the pump via the hydraulic pressure distribution unit.

摘要翻译： 车辆转向装置通过将转向电动执行器的运动传递到车轮来改变转向角。 当车辆在静止状态下转向时，液压流体可以从液压施加单元内的泵供给到转向辅助力产生液压致动器。 当车辆不处于静止状态时，液压流体可以经由液压分配单元从泵向制动装置供给。

公开(公告)号：US06470250B2

公开(公告)日：2002-10-22

申请号：US09874605

申请日：2001-06-05

申请人： Katsutoshi Nishizaki ,  Shiro Nakano ,  Masaya Segawa ,  Ryouhei Hayama ,  Kazuhiro Kato

发明人： Katsutoshi Nishizaki ,  Shiro Nakano ,  Masaya Segawa ,  Ryouhei Hayama ,  Kazuhiro Kato

IPC分类号： B62D600

CPC分类号： B60T8/246 ,  B60T8/1755 ,  B60T8/58 ,  B60T2260/02 ,  B62D6/003

摘要： A vehicle attitude control apparatus, wherein a corrected behavior index value for steering is determined in such a manner that the deviation between a target behavior index value for a vehicle and a corrected behavior index value for steering decreases, as an instability index value correlating to the amount of under-steer increases, and when the target behavior index value and the behavior index value are equal, the corrected behavior index value for steering is set to equal the behavior index value. A corrected behavior index value for braking is determined in such a manner that the deviation between the target behavior index value and a corrected behavior index value for braking increases, as the instability index value increases, and when the target behavior index value and the behavior index value are equal, the corrected behavior index value for braking is set to equal the behavior index value. The steering actuator and braking forces are controlled in such a manner that, when not in the state of under-steer, the behavior index value follows the target behavior index value, and when in the state of under-steer, the corrected behavior index value for steering and corrected behavior index value for braking follow the target behavior index value.

摘要翻译： 一种车辆姿态控制装置，其特征在于，以与所述车辆的行驶指标值相关的不稳定性指标值作为与车辆的目标行为指标值的偏差相对应的不稳定性指标值， 转向量增加，当目标行为指标值和行为指标值相等时，将转向的修正行为指标值设定为等于行为指标值。 确定用于制动的修正行为指标值，使得目标行为指数值与用于制动的修正行为指标值之间的偏差随着不稳定性指标值的增加而增加，并且当目标行为指数值和行为指数 值相等时，将用于制动的修正行为指标值设定为等于行为指标值。 控制转向执行器和制动力的方式是，当不处于转向不足的状态时，行为指标值跟随目标行为指标值，并且当处于转向不足状态时，校正行为指数值 用于制动的转向和修正行为指标值遵循目标行为指标值。

公开(公告)号：US06415215B1

公开(公告)日：2002-07-02

申请号：US09777793

申请日：2001-02-06

申请人： Katsutoshi Nishizaki ,  Shiro Nakano ,  Masaya Segawa ,  Ryouhei Hayama

发明人： Katsutoshi Nishizaki ,  Shiro Nakano ,  Masaya Segawa ,  Ryouhei Hayama

IPC分类号： B60T824

CPC分类号： B60T8/1755 ,  B60T2260/02 ,  B62D6/003 ,  B62D6/008

摘要： In a vehicle attitude control apparatus, a steering actuator is controlled so that a steering angle matches a target steering angle. In an understeer condition; the braking force on the inside wheels is increased and drive power applied to the outside wheels is larger than applied to inside wheels. In an oversteer condition, the braking force on the outside wheels is increased and drive power applied to the inside wheels is larger than applied to outside wheels. The steering actuator is controlled so that a behavior index value corresponding to the change in the vehicle'behavior, that occurs based on the change in the steering angle, matches a target behavior index value that reflects the amount of operation of an operation member. A vehicle yaw moment and an amount of control of the steering actuator vary due to steer conditions, wheel lateral slip angle and brake force control.

摘要翻译： 在车辆姿态控制装置中，转向致动器被控制成使得转向角与目标转向角匹配。 在转向不足的情况下 内轮上的制动力增加，施加到外轮的驱动力大于施加到内轮上。 在过度转向条件下，外轮上的制动力增大，施加在内侧车轮上的驱动力大于施加在外轮上。 控制转向致动器，使得与基于转向角的变化而发生的车辆行为的变化相对应的行为指标值与反映操作构件的操作量的目标行为指标值相匹配。 转向执行器的车辆横摆力矩和控制量由于转向条件，车轮横向滑移角和制动力控制而变化。

公开(公告)号：US06308122B1

公开(公告)日：2001-10-23

申请号：US09557209

申请日：2000-04-25

申请人： Katsutoshi Nishizaki ,  Takanobu Takamatsu ,  Masaya Segawa

发明人： Katsutoshi Nishizaki ,  Takanobu Takamatsu ,  Masaya Segawa

IPC分类号： B62D504

CPC分类号： B62D6/003

摘要： Motor vehicle steering system for driving a steering mechanism on the basis of an operation angle of an operation mechanism. The system includes: a behavioral variable computation section for performing computation on a behavioral variable indicative of a behavior of a motor vehicle; and a steering angle controlling section for sequentially updating a relationship between a steering angle of vehicle wheels and the operation angle of the operation mechanism on the basis of a value of the behavioral variable computed by the behavioral variable computation section and a preset target value of the behavioral variable and controlling the steering mechanism on the basis of the updated relationship. The behavioral variable may be a steady-state gain which is a steady-state ratio of the steering angle of the vehicle wheels to the operation angle of the operation mechanism. The behavioral variable may be a time constant indicative of a responsiveness of a change in the steering angle of the vehicle wheels to a change in the operation angle of the operation mechanism.

摘要翻译： 用于基于操作机构的操作角度驱动转向机构的机动车辆转向系统。 该系统包括：行为变量计算部分，用于对指示机动车辆的行为的行为变量执行计算; 以及转向角控制部，其基于由行为变量运算部计算出的行为变量的值和预定的目标值，依次更新车轮的转向角度与操作机构的操作角度之间的关系 行为变量，并根据更新的关系控制转向机制。 行为变量可以是作为车轮的转向角与操作机构的操作角度的稳态比的稳态增益。 行为变量可以是表示车轮的转向角的变化对操作机构的操作角度的变化的响应性的时间常数。