公开(公告)号：US06383393B1

公开(公告)日：2002-05-07

申请号：US08392882

申请日：1996-03-15

Applicant: Metin Colpan ,  Joachim Schorr ,  Ralf Herrmann ,  Petra Feuser

Inventor： Metin Colpan ,  Joachim Schorr ,  Ralf Herrmann ,  Petra Feuser

IPC: B01D1508

CPC classification number: C12N15/101

Abstract: A method for the purification and separation of nucleic acid mixtures by chromatography including adsorbing the nucleic acids to be separated and purified from a solution with a high concentration of salts (ionic strength) and/or a high concentration of alcohol on a substrate and subsequent desorbing from the substrate by means of a solution with lower concentration of salts (ionic strength).

Abstract translation: 一种通过色谱法纯化和分离核酸混合物的方法，其包括将从高浓度盐（离子强度）和/或高浓度醇溶液中分离和纯化的核酸吸附在底物上并随后解吸 通过具有较低浓度盐的溶液（离子强度）从底物引发。

公开(公告)号：US06020186A

公开(公告)日：2000-02-01

申请号：US442406

申请日：1995-05-16

Applicant: Karsten Henco ,  Metin Colpan ,  Petra Feuser

Inventor： Karsten Henco ,  Metin Colpan ,  Petra Feuser

IPC: C12N15/10 ,  C12Q1/68 ,  C12N3/00

CPC classification number: C12N15/101 ,  C12N15/1006 ,  C12Q1/6806

Abstract: A device and a process for isolating nucleic acids by lysing intact cells and removing nucleic acids emerging from the lysed cells by the following steps:a) the cells are immobilized in a porous matrix, with the size of matrix voids being in the range of the type of cell to be lysed;b) the cells are lysed;c) the nucleic acids are fixated on the matrix surface, and subsequentlyd) are eluted.

Abstract translation: 通过以下步骤裂解完整细胞和从裂解的细胞中除去出现的核酸来分离核酸的装置和方法：a）将细胞固定在多孔基质中，其中基质空隙的大小在 待裂解的细胞类型; b）细胞裂解; c）将核酸固定在基质表面上，随后d）被洗脱。