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    Spore state discrimination
    2.
    发明授权
    Spore state discrimination 有权

    公开(公告)号：US11650207B1

    公开(公告)日：2023-05-16

    申请号：US17206846

    申请日：2021-03-19

    申请人： Scanit Technologies, Inc.

    发明人： Pedro Manautou ,  Joel Kent

    IPC分类号： G01N33/58 ,  A61B5/15 ,  G01N21/33 ,  C12N1/14 ,  C12N3/00 ,  G01N21/64

    CPC分类号： G01N33/582 ,  A61B5/150213 ,  C12N1/14 ,  G01N21/33 ,  C12N3/00 ,  G01N2021/646 ,  G01N2333/37

    摘要： A flow of air including a fungal spore is directed to a collection cartridge. The fungal spore is trapped within the collection cartridge. The fungal spore is illuminated with ultraviolet (UV) light and a camera shutter associated with a camera sensor is opened for a time period. The camera sensor is allowed to collect light emitted from the fungal spore during a first portion of the time period. After the first portion of the time period has elapsed, a first burst of visible light originating from a first position is directed towards the fungal spore during a second portion of the time period. A second burst of visible light originating from a second position is directed towards the fungal spore. After the second portion of the time period has elapsed, the camera shutter is closed to generate an image. The image is analyzed to obtain a shape of the fungal spore.

    GENETIC CONSTRUCT AND USES THEREOF
    4.
    发明申请
    GENETIC CONSTRUCT AND USES THEREOF 有权

    公开(公告)号：US20220213491A1

    公开(公告)日：2022-07-07

    申请号：US17426092

    申请日：2020-01-28

    申请人： THE UNIVERSITY OF NOTTINGHAM

    发明人： Nigel Minton ,  Ines Canadas Blasco ,  Daphne Groothuis ,  Maria Zygouropoulou

    IPC分类号： C12N15/67 ,  A61K35/742 ,  C12N3/00

    摘要： A spore-forming cell comprising a first nucleotide sequence encoding a riboswitch and a transcriptional activator, wherein the riboswitch modulates translation of the transcriptional activator in response to a First inducer, and a second nucleotide sequence encoding a target gene that regulates spore formation, wherein the target gene is expressed in response to a second inducer and translation of the transcriptional activator. Also provided is a method of culturing a spore-forming cell and a method of creating a spore, as well as uses of spore-forming cells and spores.

    SEQUENTIAL ADDITION OF MOLECULAR GERMINANTS TO BACTERIAL SPORES
    7.
    发明申请
    SEQUENTIAL ADDITION OF MOLECULAR GERMINANTS TO BACTERIAL SPORES 审中-公开

    公开(公告)号：US20190300845A1

    公开(公告)日：2019-10-03

    申请号：US16315719

    申请日：2017-06-20

    申请人： NOVOZYMES BIOAG A/S

    发明人： Jared Heffron

    IPC分类号： C12N3/00 ,  C12N1/20 ,  C12N1/38

    摘要： We found that bacterial spores could be pretreated or initially treated with a partial complement of germinants (i.e., less germinants than would cause germination) and, subsequently, could be treated with the remaining germinants to cause germination of the spores. In some instances, the pre-treated spores germinated more efficiently than bacterial spores to which the full complement of germinants was simultaneously added to the spores.