公开(公告)号：US20130211119A1

公开(公告)日：2013-08-15

申请号：US13762489

申请日：2013-02-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Nini Kjus ,  Geir Fonnum ,  Bruce Branchaud ,  Lai-Qiang Ying ,  Steven Menchen ,  Dmitriy Gremyachinskiy ,  Hee Chol Kang

IPC: C07F7/18

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

公开(公告)号：US20130211006A1

公开(公告)日：2013-08-15

申请号：US13763066

申请日：2013-02-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven Menchen ,  Alan Blanchard ,  Luisa Andruzzi ,  Shaheer Khan ,  Dmitriy Gremyachinskiy ,  Alfred Lui ,  Craig Stolarczyk ,  Tanya Sokolsky

IPC: C08F8/40

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.

Abstract translation: 缀合基质的方法包括将与生物分子相关的抗衡离子与亲脂抗衡离子交换以形成生物分子复合物，将生物分子复合物分散在非水溶剂中，并在非水溶剂存在下将生物分子复合物偶联到底物 。