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21.发明授权
公开(公告)号：US4752584A
公开(公告)日：1988-06-21
申请号：US648794
申请日：1984-09-07
申请人： Laszlo Szemler , Eva C. Pechany
发明人： Laszlo Szemler , Eva C. Pechany
CPC分类号： C12P19/42
摘要： A fermentation process is disclosed for the preparation of an inoculum, suitable for batchwise, semicontinuous, or continuous production of coenzyme B.sub.12 under septic conditions. The fermentation process begins with a broth consisting of methanol, cornsteep liquor hydrolysate or heat-treated corn slop, ammonium bicarbonate, magnesium chloride, cobalt chloride, 5,6-dimethyl-benzimidazole and sodium bisulfite used for anaerobic, mesophilic, septic fermentation production of coenzyme B.sub.12 with anaerobic, digested sewage sludge. Fermentation is carried out over several steps to provide an inoculum containing an anaerobic, mesophilic, methane-producing, new mixed micropopulation, containing bacteria of each of the types deposited at the Hungarian National Collection of Medical Bacteria, National Institute of Hygiene, under numbers 00076, 00079, and 00272.
摘要翻译： 公开了用于制备接种物的发酵方法，适用于在脓毒症条件下分批，半连续或连续生产辅酶B12。 发酵过程开始于用于厌氧，嗜温，脓毒性发酵生产的甲醇，玉米浆水解产物或热处理玉米渣，碳酸氢铵，氯化镁，氯化钴，5,6-二甲基 - 苯并咪唑和亚硫酸氢钠的肉汤 辅酶B12与厌氧消化污水污泥。 在几个步骤上进行发酵，以提供含有厌氧，嗜温，产甲烷，新的混合微生物的接种物，其含有保藏在匈牙利国家医学细菌收集所的每种类型的细菌，国家卫生研究所，号码为00076 ，00079和00272。
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公开(公告)号：US4652527A
公开(公告)日：1987-03-24
申请号：US659199
申请日：1984-10-09
申请人： David I. Stirling
发明人： David I. Stirling
CPC分类号： C12N1/32 , C12N1/20 , Y10S435/804
摘要： This invention provides a process for single cell protein production which involves the culturing of Methylophilus methylotrophus microorganisms by facultative growth on glucose as a carbon and energy source.
摘要翻译： 本发明提供了一种单细胞蛋白质生产的方法，其涉及通过在作为碳和能量来源的葡萄糖上的兼性生长来培养甲基嗜麦芽孢杆菌微生物。
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23.发明授权
公开(公告)号：US4492756A
公开(公告)日：1985-01-08
申请号：US445783
申请日：1982-12-01
申请人： Oreste Ghisalba , Franz Heinzer , Martin Kuenzi
发明人： Oreste Ghisalba , Franz Heinzer , Martin Kuenzi
CPC分类号： C12R1/01 , C02F3/34 , C12N1/20 , C12N1/32 , Y10S435/822
摘要： The present invention relates to novel facultative methylotrophic microorganisms of the genus Hyphomicrobium, to protein-containing biomass, and to a process for the microbiological purification of aqueous solutions, e.g. wastewaters, which contain methanol, ethanol, glucose, dimethyl phosphite, trimethyl phosphite, sodium formate, sodium acetate, methylammonium chloride, dimethylammonium chloride, ethylmethylammonium chloride or, chiefly, sodium methyl sulfate, as pollutants. The respective microorganism is cultured in aqueous solution and the pollutant is degraded simultaneously.
摘要翻译： 本发明涉及新型兼性微营养微生物，涉及含蛋白质的生物质，以及涉及用于微生物学纯化水溶液的方法。 含有甲醇，乙醇，葡萄糖，亚磷酸二甲酯，亚磷酸三甲酯，甲酸钠，乙酸钠，甲基氯化铵，二甲基氯化铵，乙基甲基氯化铵或主要是甲基硫酸钠作为污染物的废水。 将各微生物在水溶液中培养，同时污染物降解。
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公开(公告)号：US4439525A
公开(公告)日：1984-03-27
申请号：US300521
申请日：1981-09-09
申请人： Lucas K. Shay , Eugene H. Wegner
发明人： Lucas K. Shay , Eugene H. Wegner
CPC分类号： C12R1/84 , C12N1/16 , C12N1/32 , C12P13/12 , Y10S435/804 , Y10S435/938
摘要： Mutant yeasts of the strain Pichia pastoris have been developed which contain relatively high levels of methionine. These high methionine content Pichia pastoris mutants grow on an oxygenated hydrocarbon such as methanol, to produce improved amino acid balance single-cell protein product eliminating or reducing the need to supplement single-cell protein with methionine when used as food supplements.
摘要翻译： 已经开发了含有相对高水平甲硫氨酸的巴斯德毕赤酵母菌株的突变酵母。 这些高甲硫氨酸含量的巴斯德毕赤酵母突变体在含氧的烃如甲醇上生长，以产生改善的氨基酸平衡单细胞蛋白产物，当用作食品补充剂时，消除或减少了用甲硫氨酸补充单细胞蛋白的需要。
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公开(公告)号：US4430429A
公开(公告)日：1984-02-07
申请号：US286247
申请日：1981-07-23
CPC分类号： C12R1/01 , C12P19/42 , Y10S435/822
摘要： Vitamin B.sub.12 -active substances are produced by the fermentation of an aqueous medium consisting essentially of methanol as the main source of assimilable carbon, a source of assimilable nitrogen, cobalt ions and essential growth factors and minerals with a vitamin B.sub.12 -activity substance producing organism capable of growing under anaerobic conditions.
摘要翻译： 维生素B12活性物质通过发酵基本上由甲醇作为可同化碳的主要来源，可同化的氮，钴离子和必需生长因子的来源以及具有维生素B12活性物质产生生物体的矿物质的水性培养基 在无氧条件下生长。
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公开(公告)号：US4427774A
公开(公告)日：1984-01-24
申请号：US358952
申请日：1982-03-17
CPC分类号： C12P13/08 , Y10S435/822
摘要： L-threonine is produced by aerobically culturing a bacterium belonging to the genus Acinetobacter, which utilizes ethanol and has an ability to produce and accumulate L-threonine, in a culture medium in which ethanol is the main carbon source to produce and accumulate L-threonine in a culture liquor and recovering L-threonine from the culture liquor. The bacterium is preferably a mutant of Acinetobacter calcoaceticus YK-1011 (Ferm-P No. 5910).
摘要翻译： 通过有氧培养属于不动杆菌属的细菌产生L-苏氨酸，其在乙醇为主要碳源的培养基中利用乙醇并具有产生和积累L-苏氨酸的能力，以产生和积累L-苏氨酸 在培养液中并从培养液中回收L-苏氨酸。 细菌优选为不动杆菌乙酸杆菌YK-1011（Ferm-P No.5910）的突变体。
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公开(公告)号：US4268630A
公开(公告)日：1981-05-19
申请号：US25711
申请日：1979-03-30
CPC分类号： C12N9/0006 , C12P7/26 , C12P7/28 , Y10S435/822 , Y10S435/858
摘要： A process is disclosed for the microbiological production of ketones from C.sub.3 -C.sub.6 alkanes by contacting C.sub.3 -C.sub.6 alkanes under aerobic conditions with resting microbial cells derived from a methylotrophic microorganism or enzyme preparation derived from said cells, wherein the microorganism has been previously grown under aerobic conditions in a nutrient medium containing methane or dimethyl ether.
摘要翻译： 公开了一种用于通过在有氧条件下将C3-C6烷烃与衍生自衍生自所述细胞的甲基营养微生物或酶制剂的静息微生物细胞接触的C3-C6烷烃的微生物生产的方法，其中所述微生物已经在有氧条件下生长 含有甲烷或二甲醚的营养培养基中的条件。
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公开(公告)号：US4261420A
公开(公告)日：1981-04-14
申请号：US34796
申请日：1979-04-30
申请人： Donald O. Hitzman
发明人： Donald O. Hitzman
CPC分类号： C09K8/905 , C12N1/32 , E21B43/164 , Y02P90/70 , Y10S435/804 , Y10S435/923 , Y10S435/924 , Y10S435/93 , Y10S435/938
摘要： A single cell protein plant is operated to produce high density cell growth and a substantially pure stream of generally high pressure carbon dioxide for further use, for example, in enhanced oil recovery operations. The plant employs an air separator producing substantially pure streams of oxygen and nitrogen. The oxygen stream is used to enrich a carrier fluid and used for aeration of the fermenter. The off-gases from the fermenter are separated into a generally high pressure, substantially pure carbon dioxide stream which can be used for enhanced oil recovery and a residual recycle stream to which oxygen is again added and which is returned to the fermenter. The single cell protein is dried and further processed as required for human or animal consumption.
摘要翻译： 操作单细胞蛋白质植物以产生高密度细胞生长和基本上纯的一般高压二氧化碳流，用于进一步使用，例如在增强的采油操作中。 该工厂使用空气分离器，产生基本上纯净的氧气和氮气流。 氧气流用于富集载体流体并用于发酵罐的通气。 来自发酵罐的废气被分离成通常高压，基本上纯的二氧化碳流，其可用于增强油采收和再次添加氧并将其返回到发酵罐的残余循环物流。 将单细胞蛋白质干燥并根据人或动物消费的需要进一步加工。
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29.发明授权Method for growing pseudomycelial yeasts and reducing bacterial contamination in a yeast fermentation process 失效在酵母发酵过程中培养假菌丝体酵母并减少细菌污染的方法
公开(公告)号：US4189538A
公开(公告)日：1980-02-19
申请号：US917896
申请日：1978-06-22
CPC分类号： C12N1/32 , C12N1/02 , C12N1/34 , Y10S435/804 , Y10S435/813
摘要： Growth of pseudomycelial yeasts and removal of bacterial contamination in conventional fermentation processes are accomplished by withdrawing product as a foam from the fermentor.
摘要翻译： 在常规发酵过程中，假丝状酵母的生长和细菌污染的去除是通过从发酵罐中抽出产物作为泡沫来实现的。
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30.发明授权Process for the preparation of single cell protein using Methylmonas clara ATCC 31226 失效使用{i Methylmonas clara {b ATCC 31226制备单细胞蛋白的方法
公开(公告)号：US4166004A
公开(公告)日：1979-08-28
申请号：US817870
申请日：1977-07-21
申请人： Paul Prave , Dieter Sukatsch , Uwe Faust
发明人： Paul Prave , Dieter Sukatsch , Uwe Faust
CPC分类号： C12N1/32 , Y10S435/804 , Y10S435/822
摘要： A process for the preparation of a biomass is disclosed by cultivation of bacteria of the genus Methylomonas under aerobic conditions in a nutrient medium containing methanol as the sole carbon source, nitrogen sources and essential mineral salts, which comprises using a strain of the species Methylomonas clara ATCC 31226. The single cell protein thus obtained has a low content of nucleic acids, fats and carbohydrates and is especially suitable to prepare food and feed.
摘要翻译： 通过在需氧条件下在含有甲醇作为唯一碳源，氮源和必需矿物盐的营养培养基中培养Methylomonas属细菌，公开了生物质的制备方法，该方法包括使用Methylomonas clara 由此获得的单细胞蛋白具有低含量的核酸，脂肪和碳水化合物，并且特别适用于制备食物和饲料。
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