METHOD FOR DETERMINING PROTEIN STRUCTURE USING CRYO-ELECTRON MICROSCOPY

    公开(公告)号:US20230349847A1

    公开(公告)日:2023-11-02

    申请号:US18307936

    申请日:2023-04-27

    CPC classification number: G01N23/2251 G01N23/2202

    Abstract: A method for determining a protein structure using cryo-electron microscopy, including: enabling a target protein to contain a tag; enabling a resulting target containing the tag to bind a scaffold protein to form a complex between the target protein and the scaffold protein; and performing single-particle imaging using the cryo-electron microscopy to determine a structure of the target protein in complex with the scaffold protein. The scaffold protein is any one of streptavidin, avidin, or derivatives thereof. The tag is configured for selectively binding to the scaffold protein. The tag is one selected from the group consisting of: a biotin tag, comprising a biotin; a biotinylated protein or polypeptide tag, comprising a protein sequence and a biotin covalently linked to the protein sequence; a Strep-tag; and a biotinylated or strep-tagged antibody, or antibody Fab fragment, or single-chain antibody.

    Atrial fibrillation signal recognition method, apparatus and device

    公开(公告)号:US11538588B2

    公开(公告)日:2022-12-27

    申请号:US16901033

    申请日:2020-06-15

    Abstract: The present disclosure provides an atrial fibrillation signal recognition method, apparatus and device. The method comprises: obtaining an electrocardiogram signal to be recognized; inputting the electrocardiogram signal to be recognized to a pre-established atrial fibrillation signal recognition model, and outputting an atrial fibrillation signal recognition result, where the atrial fibrillation signal recognition model is established in the following way: obtaining a specified number of electrocardiogram sample signals and corresponding identifier information; balancing, according to the number of normal signals, atrial fibrillation signals by means of SMOTE; establishing a network structure of multiple convolutional neural networks, each of the convolutional neural networks being provided with a specific receptive field for recognizing the atrial fibrillation signals of a corresponding granularity; and inputting the normal signals and the balanced atrial fibrillation signals to the network structure for training to generate an atrial fibrillation signal recognition model.

    FLEXIBLE CONDUCTIVE THIN FILM BASED ON SILVER POWDER AND PDMS, AND PREPARATION METHOD THEREFOR

    公开(公告)号:US20220076858A1

    公开(公告)日:2022-03-10

    申请号:US17413292

    申请日:2019-04-10

    Abstract: The invention applies to the technical field of the flexible conductive film and provides a stretchable and super-sensitive flexible conductive film based on silver powder and PDMS and its manufacturing method. The manufacturing method comprises the following steps: preparing silver powder, PDMS prepolymer, and the said PDMS curing agent; after the said silver powder is ground, cleaned, and dried, adding the said PDMS prepolymer for even mixing, and then adding the said PDMS curing agent for mixing to get the liquid flexible conductive film; spin-coating the said liquid flexible conductive film to a certain thickness and get the flexible conductive film after curing. The flexible conductive film comprises silver powder and PDMS film substrate, wherein the said powder silver is evenly distributed within the said PDMS film substrate. The invention provides a flexible conductive film based on silver powder and PDMS and its manufacturing method, whose manufacturing processes are simple and free of hazardous chemical reagents, raising lower requirements for the device but performing well in conductivity and stretchability.

    Three-degree-of-freedom parallel mechanism with curved sliding pairs

    公开(公告)号:US10994410B2

    公开(公告)日:2021-05-04

    申请号:US16479920

    申请日:2017-04-25

    Abstract: A three-degree-of-freedom parallel mechanism with curved sliding pairs includes a fixed platform, a moving platform, and three curved branches disposed between the fixed platform and the moving platform. Each of the curved branches includes a first curved link and a second curved link that share a common arc center. One end of the first curved link is connected to fixed platform by a rotational pair. One end of the second curved link is disposed in a cavity at another end of the first curved link. The second curved link is operative to perform a reciprocating motion along a tangent of an arc of the first curved link. Another end of the second curved link is connected to the moving platform by a ball joint. The axes of the three rotational pairs of the three curved branches coincide with each other and are perpendicular to the fixed platform. In the three-degree-of-freedom parallel mechanism with curved sliding pairs, the moving platform of the parallel mechanism is rotatable around the X-axis, Y-axis, and Z-axis of a three-dimensional coordinate system taking the arc center of the three curved branches as the origin, where the rotation of the moving platform about the Z axis is decoupled from the rotation in the other two orientations.

    Method and apparatus for extracting centerline of tubular object

    公开(公告)号:US10818027B2

    公开(公告)日:2020-10-27

    申请号:US16077523

    申请日:2017-05-24

    Abstract: A method and an apparatus for extracting a centerline of a tubular object are provided. The method includes: preprocessing an image of the tubular object whose centerline is to be extracted to obtain an enhanced image, acquiring a gradient vector flow field of the enhanced image; acquiring a deformation force parameter based on the gradient vector flow field and extracting ridge points of the tubular object from the enhanced image, establishing a regularized open curve deformable model; if a deformation end condition is not met, deforming and processing one ridge line segment in the initial ridge line segment list based on the model to obtain a centerline segment of the tubular object, updating the initial ridge line segment list based on the ridge line segment traversed; if the deformation end condition is met, generating a centerline of the tubular object based on the centerline segments.

    ULTRASOUND PROBE CALIBRATION PHANTOM, ULTRASOUND PROBE CALIBRATION SYSTEM AND CALIBRATION METHOD THEREOF

    公开(公告)号:US20170245837A1

    公开(公告)日:2017-08-31

    申请号:US15519531

    申请日:2015-12-31

    CPC classification number: A61B8/587 A61B8/4254 A61B8/4444

    Abstract: An ultrasound probe calibration system and method. The system comprises an ultrasound probe calibration phantom (100), and the ultrasound probe calibration phantom (100) is provided with a sunken recess (110) at a middle position of an upper surface thereof and with several conical holes on a side surface thereof. The sunken recess (110) is fixedly connected with a two dimensional ultrasound probe (220) therein. The conical hole is inserted with an NDI insertion stylus (230), and a tip of the NDI insertion stylus (230) can be acquired in ultrasound imaging. An ultrasound probe calibration system employing the above structure enables a midplane of an ultrasound plane to pass a midplane of an ultrasound probe calibration phantom (100) along a middle gap, such that a tip of an NDI insertion stylus (230) is used for the midplane of the ultrasound plane, thereby addressing the problem of misalignment of a point-type phantom or a two-dimensional plane-type phantom to the ultrasound plane.

    GPU-BASED PARALLEL ELECTROCARDIOGRAM SIGNAL ANALYSIS METHOD, COMPUTER READABLE STORAGE MEDIUM AND DEVICE

    公开(公告)号:US20170105643A1

    公开(公告)日:2017-04-20

    申请号:US15389978

    申请日:2016-12-23

    Abstract: The present disclosure provides a GPU-based parallel electrocardiogram signal analysis method, comprising: performing a filtering process of electrocardiogram signals through a long interval artifact removal and a short interval artifact removal; performing a QRS detection of the filtering-processed electrocardiogram signals through an R-wave position extraction, a QRS complex start and end positions extraction and a QRS complex width extraction; performing an abnormal waveform classification of the QRS-detected electrocardiogram signals through template creation; wherein at least one of the long interval artifact removal, the short interval artifact removal, the R-wave position extraction, the QRS complex width extraction and the creation template is performed by a multiple threads at a GPU device side in parallel, any thread being read through its unique index number to process corresponding data. By executing one or more steps of the electrocardiogram signal analysis at GPU in parallel, the present disclosure increases the analysis speed of the electrocardiogram signals.

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