公开(公告)号：US20210396745A1

公开(公告)日：2021-12-23

申请号：US17462842

申请日：2021-08-31

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Alexander Green ,  Duo Ma ,  Luhui Shen ,  Chris Diehnelt

IPC: G01N33/543 ,  G01N33/569 ,  C07K19/00 ,  G01N33/53

Abstract: Provided herein are methods and systems for low-cost, low-equipment detection of pathogens in biological sample. In particular, provided herein is a low-cost method for detecting norovirus that provides reliable, visible test with femtomolar, attomolar, and zeptomolar detection limits and that uses materials suitable for deployment of the methods in the field.

公开(公告)号：US20210348222A1

公开(公告)日：2021-11-11

申请号：US17324944

申请日：2021-05-19

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Alexander Green

IPC: C12Q1/6841 ,  C12N15/115 ,  G01N33/53

Abstract: Provided herein are imaging probes and systems and methods employing such imaging probes for real-time, label-free, multiplexed imaging of RNAs in living cells. More particularly, aptamer-based sensors (“aptasensors”) and molecular fuses comprising multiple aptasensors are genetically encoded imaging probes comprising RNA-target binding sequence and an intramolecular reconfiguration sequence. The probe is configured such that binding of a RNA target by the RNA-target binding sequence triggers the intramolecular reconfiguration sequence to reconfigure such that an optically detectable output is generated by the probe.

公开(公告)号：US10941401B2

公开(公告)日：2021-03-09

申请号：US16322799

申请日：2017-08-01

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Alexander Green ,  Yu Zhou

IPC: C12N15/113 ,  C12N15/67 ,  H03K19/20

Abstract: Provided herein are synthetic nucleic acid molecules and methods of using such synthetic nucleic acid molecules for strong repression of target gene expression. In particular, provided herein are methods for altering expression of a protein in a cell, where the method comprises introducing into a cell a protein coding sequence operably linked to a near-threshold translational repressor having first and second trigger recognition sequences that are fully or partially complementary to a repressing trigger RNA; and introducing into a cell the repressing trigger RNA.

公开(公告)号：US20180354806A1

公开(公告)日：2018-12-13

申请号：US15775735

申请日：2016-11-11

Applicant: Arizona Board of Regents on Behalf of Arizona State University

Inventor： Alexander Green ,  Ahmed Yousaf ,  Abhishek Debnath

IPC: C01B35/04 ,  C08J3/215 ,  C08J5/18 ,  C08K3/38

Abstract: Some embodiments include a method of producing metal diboride nanomaterials having thickness down to the atomic scale and lateral areas from 10 nm to over 1 μm by preparing a mixture of a metal diboride and a suspending solution. The suspending solution can be an organic solvent or a solution containing water, and optionally can include a dispersion agent, such as a surfactant, a polymer, small molecule, or biopolymer. Further, the method includes exfoliating the metal diboride by exposing the mixture to ultrasonic energy, centrifuging the mixture forming supernatant that includes a dispersion of exfoliated metal diborides, and extracting the dispersion from the supernatant. Some embodiments include extracting the supernatant and casting the solution by diluting the dispersion with a second suspending solution that includes dissolved polymer. This can result in a composite film includes a dispersion of the exfoliated metal diborides and provides improved mechanical properties.