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1.发明授权公开(公告)号：US11952637B2
公开(公告)日：2024-04-09
申请号：US18001395
申请日：2021-06-11
Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY , THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO
Inventor： Alexander Green , Keith Pardee , Margot Karlikow , Kaiyue Wu , Masoud Norouzi
IPC: C12Q1/70 , C12Q1/6897
CPC classification number: C12Q1/6897 , C12Q1/701
Abstract: Provided herein are methods and compositions for rapid, highly sensitive detection of SARS-CoV-2 in biological samples. In particular, provided herein is a rapid, low-cost method for detecting SARS-CoV-2 that provides reliable, visible test results and does not require PCR reagents, elaborate biosafety precautions, or sophisticated laboratory equipment.
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公开(公告)号：US20220372586A1
公开(公告)日：2022-11-24
申请号：US17879871
申请日：2022-08-03
Inventor： Alexander Green , Fan Hong , Soma Chaudhary , Anli Tang , Hao Yan
IPC: C12Q1/6897 , C12Q1/6858
Abstract: Ultraspecific, programmable nucleic acid sensors capable of detecting and preferentially amplifying target DNA molecules comprising a particular SNP or mutation are provided. In some cases, the ultraspecific programmable nucleic acid sensors are useful for detecting SNP-containing DNA molecules indicative of cancer such as cell-free DNA circulating in the blood or indicative of organ transplant rejection Also provided are methods for construction of such ultraspecific nucleic acid sensors and methods for preferential amplification of target DNA molecules containing a mutation of interest, as well as testing systems for early cancer screening and routine monitoring of circulating cancer DNA using liquid biological samples such as serum, plasma, or saliva.
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公开(公告)号：US20210289791A1
公开(公告)日：2021-09-23
申请号：US17264766
申请日：2019-07-30
Inventor： Alexander Green , Qing Hua Wang , Sanchari Saha , Abhishek Debnath
Abstract: Biocompatible polymer-coated transition metal chalcogenide (TMC) nanomaterials are provided herein. In particular, provided herein are two-dimensional polymer-coated TMC nanomaterials having excellent antimicrobial properties and biocompatibility, as well as methods of inhibiting microbiological growth on, or in, devices coated by or otherwise comprising the biocompatible polymer-coated transition metal chalcogenide (TMC) nanomaterials. In some cases, the biopolymer coating encapsulating the TMC nanomaterial comprises short synthetic single-stranded DNAs (ssDNAs). As described herein, ssDNA-encapsulated TMDCs exhibit no cytotoxicity against human cell lines at concentrations up to 0.25 mg/mL, but they exhibit exceptionally strong bactericidal activity against both gram-positive and gram-negative bacteria, including antibiotic-resistant Escherichia coli and a gram-positive methicillin-resistant Staphylococcus aureus (MRSA) strain. In other cases, TMDCs encapsulated by poly-L-lysine and Pluronic F77 display strong activity against multi drug resistance bacteria and form coatings that strongly inhibit bacterial biofilms, while TMDCs encapsulated by chitosan exhibit strong activity against fungi.
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公开(公告)号：US20200080137A1
公开(公告)日：2020-03-12
申请号：US16303937
申请日：2017-05-25
Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY , PRESIDENT AND FELLOWS OF HARVARD COLLEGE , MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Inventor： Alexander Green , Dana Braff , Melissa K. Takahashi , Keith Pardee , James J. Collins , Guillaume Lambert , Thomas Ferrante
IPC: C12Q1/6827 , C12Q1/6816 , C12Q1/70
Abstract: Methods for detecting the presence of a pathogen infection are described. In particular, this document provides a method of detecting target nucleic acids, such as pathogen-specific RNA, in a biological sample obtained from a subject, where the method comprises using one or more toehold switch sensors and an isothermal amplification step to detect the target nucleic acid. Methods specific for detecting and identify the presence of a virus such as Zika virus are also provided.
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公开(公告)号：US20240052400A1
公开(公告)日：2024-02-15
申请号：US18453129
申请日：2023-08-21
Inventor： Alexander Green , Duo Ma , Anli Tang
IPC: C12Q1/6825 , C12Q1/6816
CPC classification number: C12Q1/6825 , C12Q1/6816 , Y02A50/30
Abstract: Methods and compositions for detecting the presence of a pathogen are described. In particular, this document provides a method of detecting a pathogen-associated nucleic acid in a biological sample of a subject, where the method comprises using one or more unimolecular aptamer-based sensors comprising an aptamer-fluorophore complex and an amplification step to detect the pathogen-associated nucleic acid. Methods specific for detecting the presence of malaria and other mosquito-borne virus infections are also provided.
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Patent Agency Ranking
6.发明公开公开(公告)号：US20240043853A1
公开(公告)日：2024-02-08
申请号：US18311587
申请日：2023-05-03
Inventor： Alexander Green , Fan Hong
IPC: C12N15/67 , C12Q1/6825 , C12Q1/6897
CPC classification number: C12N15/67 , C12Q1/6825 , C12Q1/6897
Abstract: Provided herein are highly sensitive compositions and methods for detection of at least one specific nucleic acid molecule in a sample. The presence of a specific nucleic acid provides a positive indicator of a pathogenic agent, contaminant, non-canonical bases, and/or wild-type or mutated genes in a sample or a cell. Applications for which the compositions and methods are particularly well suited include point-of-care disease diagnosis or cellular RNA imaging.
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公开(公告)号：US11680269B2
公开(公告)日：2023-06-20
申请号：US16322719
申请日：2017-08-01
Inventor： Alexander Green , Fan Hong
IPC: C12N15/67 , C12Q1/6825 , C12Q1/6897
CPC classification number: C12N15/67 , C12Q1/6825 , C12Q1/6897
Abstract: Provided herein are highly sensitive compositions and methods for detection of at least one specific nucleic acid molecule in a sample. The presence of a specific nucleic acid provides a positive indicator of a pathogenic agent, contaminant, non-canonical bases, and/or wild-type or mutated genes in a sample or a cell. Applications for which the compositions and methods are particularly well suited include point-of-care disease diagnosis or cellular RNA imaging.
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公开(公告)号：US11125743B2
公开(公告)日：2021-09-21
申请号：US16279045
申请日：2019-02-19
Inventor： Alexander Green , Duo Ma , Luhui Shen , Chris Diehnelt
IPC: G01N33/543 , G01N33/53 , G01N33/569 , C07K19/00
Abstract: Provided herein are methods and systems for low-cost, low-equipment detection of pathogens in biological sample. In particular, provided herein is a low-cost method for detecting norovirus that provides reliable, visible test with femtomolar, attomolar, and zeptomolar detection limits and that uses materials suitable for deployment of the methods in the field.
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9.发明申请公开(公告)号：US20190285620A1
公开(公告)日：2019-09-19
申请号：US16279045
申请日：2019-02-19
Inventor： Alexander Green , Duo Ma , Luhui Shen , Chris Diehnelt
IPC: G01N33/543 , G01N33/569 , C07K19/00 , G01N33/53
Abstract: Provided herein are methods and systems for low-cost, low-equipment detection of pathogens in biological sample. In particular, provided herein is a low-cost method for detecting norovirus that provides reliable, visible test with femtomolar, attomolar, and zeptomolar detection limits and that uses materials suitable for deployment of the methods in the field.
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10.发明申请公开(公告)号：US20190276901A1
公开(公告)日：2019-09-12
申请号：US16294578
申请日：2019-03-06
Inventor： Alexander Green
IPC: C12Q1/6895 , C12Q1/6897 , C12Q1/6837
Abstract: Provided herein are methods and compositions for rapid, highly sensitive detection of Valley fever in biological samples. In particular, provided herein is a low-cost method for detecting Valley fever that provides reliable, visible test results and does not require elaborate biosafety precautions or sophisticated laboratory equipment.
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