公开(公告)号：US20220236164A1

公开(公告)日：2022-07-28

申请号：US17545646

申请日：2021-12-08

Applicant: Becton, Dickinson and Company

Inventor： Matthew Bahr ,  Christopher Ghazi ,  Keegan Owsley

IPC: G01N15/14 ,  G01F1/661

Abstract: Aspects of the present disclosure include methods for determining drop delay of a particle in a flow stream (e.g., in a particle analyzer). Methods according to certain embodiments include irradiating the particle in the flow stream with two or more spatially separated lasers, detecting light from the particle in a first photodetector channel and a second photodetector channel calculating a velocity of the particle in the flow stream based on the detected light in the first photodetector channel and the second photodetector channel and determining the drop delay of the particle based on the calculated velocity. Systems (e.g., particle analyzers) having a light source with two or more spatially separated lasers and a light detection system for practicing the subject methods are also described. Integrated circuits and non-transitory computer readable storage medium having instructions stored thereon for determining drop delay according to the subject methods are also provided.

公开(公告)号：US11328422B2

公开(公告)日：2022-05-10

申请号：US16817135

申请日：2020-03-12

Applicant: Becton, Dickinson and Company

Inventor： Matthew Bahr ,  Keegan Owsley

IPC: G06K9/00 ,  G06T7/00 ,  G01N15/14 ,  G01N21/64 ,  C12Q1/02

Abstract: Disclosed herein include embodiments of a system, a device, and a method for sorting a plurality cells of a sample. A plurality of raw images comprising pixels of complex values in a frequency space can be generated from a plurality of channels of fluorescence intensity data of fluorescence emissions of fluorophores, the fluorescence emissions being elicited by fluorescence imaging using radiofrequency-multiplexed excitation in a temporal space. Spectral unmixing can be performed on the raw images prior to a sorting decision being made.

公开(公告)号：US20200378882A1

公开(公告)日：2020-12-03

申请号：US16887538

申请日：2020-05-29

Applicant: Becton, Dickinson and Company

Inventor： Jonathan Lin ,  Keegan Owsley ,  Matthew Bahr

IPC: G01N15/14

Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.

公开(公告)号：US20200309664A1

公开(公告)日：2020-10-01

申请号：US16817331

申请日：2020-03-12

Applicant: Becton, Dickinson and Company

Inventor： Matthew Bahr ,  Eric D. Diebold ,  Jonathan Lin ,  Keegan Owsley

IPC: G01N15/02 ,  G01N15/14

Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image of an object in the flow stream in an interrogation region and determining whether the object in the flow stream is an aggregate based on the generated image. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image of an object in a flow stream and to determine whether the object is an aggregate are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.

公开(公告)号：US20200302606A1

公开(公告)日：2020-09-24

申请号：US16817135

申请日：2020-03-12

Applicant: Becton, Dickinson and Company

Inventor： Matthew Bahr ,  Keegan Owsley

IPC: G06T7/00 ,  G01N15/14 ,  G01N21/64 ,  C12Q1/02

Abstract: Disclosed herein include embodiments of a system, a device, and a method for sorting a plurality cells of a sample. A plurality of raw images comprising pixels of complex values in a frequency space can be generated from a plurality of channels of fluorescence intensity data of fluorescence emissions of fluorophores, the fluorescence emissions being elicited by fluorescence imaging using radiofrequency-multiplexed excitation in a temporal space. Spectral unmixing can be performed on the raw images prior to a sorting decision being made.

公开(公告)号：US20180073974A1

公开(公告)日：2018-03-15

申请号：US15698506

申请日：2017-09-07

Applicant: Becton, Dickinson and Company

Inventor： Eric D. Diebold ,  Keegan Owsley ,  Matthew Bahr

IPC: G01N15/14 ,  G02F1/33 ,  H01S3/00

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N2015/1006 ,  G01N2015/145 ,  G02F1/33 ,  G02F2203/18 ,  G02F2203/24 ,  H01S3/0071

Abstract: Aspects of the present disclosure include methods for producing an output laser beam having two or more angularly deflected laser beams (e.g., for irradiating a sample in a flow stream) with a predetermined intensity profile. Systems for practicing the subject methods having a laser, an acousto-optic device, a radiofrequency generator and a controller for adjusting the amplitude of the radiofrequency drive signals to produce an output laser beam of angularly deflected laser beams with a predetermined intensity profile are also described.