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    MULTIPLEX FLUORESCENT PARTICLE DETECTION USING SPATIALLY DISTRIBUTED EXCITATION
    5.
    发明申请
    MULTIPLEX FLUORESCENT PARTICLE DETECTION USING SPATIALLY DISTRIBUTED EXCITATION 有权
    使用空间分布激励的多重荧光颗粒检测

    公开(公告)号：US20140313510A1

    公开(公告)日：2014-10-23

    申请号：US14124589

    申请日：2012-06-04

    Applicant: Holger Schmidt ,  Aaron Roe Hawkins

    Inventor： Holger Schmidt ,  Aaron Roe Hawkins

    IPC: G01N21/64 ,  G01B9/02 ,  G01N21/49

    CPC classification number: G01N21/6486 ,  G01B9/02041 ,  G01N15/1436 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/05 ,  G01N21/49 ,  G01N21/53 ,  G01N21/645 ,  G01N2015/1006 ,  G01N2015/145 ,  G01N2021/0346 ,  G01N2021/058

    Abstract: A chip-scale optical approach to performing multi-target detection is based on molecular biosensing using fiber-optic based fluorescence or light scattering detection in liquid-core waveguides. Multiplexing methods are capable of registering individual nucleic acids and other optically responsive particles, and are ideal for amplification-free detection in combination with the single molecule sensitivity of optofluidic chips. This approach overcomes a critical barrier to introducing a new integrated technology for amplification-free molecular diagnostic detection. Specific examples of liquid-core optical waveguides and multi-mode interferometers are described; however, they can be implemented in a number of different ways as long as a series of excitation spots is created whose spacing varies with the excitation wavelength.

    Abstract translation: 执行多目标检测的芯片级光学方法基于在液芯波导中使用基于光纤的荧光或光散射检测的分子生物传感。 复用方法能够记录单个核酸和其他光学响应性颗粒，并且是与光流体芯片的单分子灵敏度相结合的无扩增检测的理想选择。 该方法克服了引入新的无扩增分子诊断检测技术的关键障碍。 描述了液芯光波导和多模干涉仪的具体实例; 然而，它们可以以多种不同的方式实现，只要产生一系列激发点，其间距随着激发波长而变化。

    Quantitative two-photon flow cytometry
    6.
    发明授权
    Quantitative two-photon flow cytometry 有权
    定量双光子流式细胞术

    公开(公告)号：US08486371B2

    公开(公告)日：2013-07-16

    申请号：US11662835

    申请日：2005-09-16

    Applicant: Jing Yong Ye ,  Theodore B. Norris ,  Cheng Frank Zhong ,  James R. Baker

    Inventor： Jing Yong Ye ,  Theodore B. Norris ,  Cheng Frank Zhong ,  James R. Baker

    IPC: A61K49/00

    CPC classification number: G01N15/1459 ,  G01N15/1404 ,  G01N15/1484 ,  G01N2015/1075 ,  G01N2015/145 ,  G01N2015/1472 ,  G01N2015/1493

    Abstract: A method and apparatus of multi-dye analysis of particles using flow cytometer. The method includes dying particles to be detected using two or more dyes; urging the particles through a capillary in a non-uniform flow; exciting a first of the particles within the capillary using a multiphoton excitation laser beam causing the two or more dyes each to fluoresce thereby producing a first output signal and a second output signal respectively; and detecting the first output signal and the second output signal. A second of the particles within the capillary being excited using the multiphoton excitation laser beam causing the two or more dyes each to fluoresce thereby producing a third output signal and a forth output signal respectively. The method finally includes comparing a ratio of the first output signal and the second output signal to a ratio of the third output signal and the forth output signal to detect a desired change in the particles.

    Abstract translation: 使用流式细胞仪对颗粒进行多染色分析的方法和装置。 该方法包括使用两种或更多种染料染色待检测的颗粒; 以不均匀的流动促使颗粒通过毛细管; 使用多光子激发激光束激发毛细管内的第一颗粒，使得两种或更多种染料各自发荧光，从而分别产生第一输出信号和第二输出信号; 以及检测第一输出信号和第二输出信号。 使用多光子激发激光束激发毛细管内的第二颗粒，使得两种或更多种染料各自发出荧光，从而分别产生第三输出信号和第四输出信号。 该方法最后包括将第一输出信号和第二输出信号的比率与第三输出信号和第四输出信号的比率进行比较，以检测颗粒中期望的变化。

    OPTICALLY INTEGRATED MICROFLUIDIC CYTOMETERS FOR HIGH THROUGHPUT SCREENING OF PHOTOPHYSICAL PROPERTIES OF CELLS OR PARTICLES
    7.
    发明申请
    OPTICALLY INTEGRATED MICROFLUIDIC CYTOMETERS FOR HIGH THROUGHPUT SCREENING OF PHOTOPHYSICAL PROPERTIES OF CELLS OR PARTICLES 有权
    用于高通量筛选细胞或颗粒的光学性质的光学综合微流感测定仪

    公开(公告)号：US20120241643A1

    公开(公告)日：2012-09-27

    申请号：US13360706

    申请日：2012-01-28

    Applicant: Amy E. Palmer ,  Ralph Jimenez ,  Kevin Dean ,  Jennifer Lubbeck ,  Lloyd M. Davis

    Inventor： Amy E. Palmer ,  Ralph Jimenez ,  Kevin Dean ,  Jennifer Lubbeck ,  Lloyd M. Davis

    IPC: G01N21/64

    CPC classification number: G01N15/1436 ,  G01N15/1459 ,  G01N15/1484 ,  G01N2015/1006 ,  G01N2015/145

    Abstract: Devices and methods for screening emissive properties of a cell, such as the resistance to photobleaching or other photophysical property. In one example, a device may include a microfluidic reservoir having at least an input channel for receiving the cell, a main channel fluidly coupled with the input channel, at least a first output channel and a second output channel, the first and second output channels fluidly coupled with the main channel; and a multibeam interrogation section generating a plurality of light beams impinging upon the main channel of the microfluidic reservoir. As a cell passes from the input channel through the main channel of the microfluidic reservoir, the cell is exposed to the plurality of light beams thereby generating emissions that are received by a signal processing section. A cell trapping section selectively diverts the cell to the second output channel if the cell contains desired emissive properties.

    Abstract translation: 用于筛选细胞的发射性质的装置和方法，例如抗光漂白或其它光物理性质。 在一个示例中，设备可以包括具有至少一个用于接收单元的输入通道的微流体储存器，与输入通道流体耦合的主通道，至少第一输出通道和第二输出通道，第一和第二输出通道 与主通道流体耦合; 以及多光束询问部分，其产生撞击在微流体储存器的主通道上的多个光束。 当细胞从输入通道通过微流体储存器的主通道时，电池暴露于多个光束，从而产生由信号处理部分接收的发射。 如果单元包含期望的发射特性，则细胞俘获部分选择性地将细胞转移到第二输出通道。

    Particle measurement systems and methods
    8.
    发明授权
    Particle measurement systems and methods 有权
    粒子测量系统和方法

    公开(公告)号：US08031339B2

    公开(公告)日：2011-10-04

    申请号：US12335408

    申请日：2008-12-15

    Applicant: Paul T. Steele

    Inventor： Paul T. Steele

    IPC: G01N15/02

    CPC classification number: G01N15/1459 ,  G01N21/47 ,  G01N21/645 ,  G01N2015/1075 ,  G01N2015/145 ,  G01N2015/1493 ,  G01N2021/4709

    Abstract: A system according to one embodiment includes a light source for generating light fringes; a sampling mechanism for directing a particle through the light fringes; and at least one light detector for detecting light scattered by the particle as the particle passes through the light fringes. A method according to one embodiment includes generating light fringes using a light source; directing a particle through the light fringes; and detecting light scattered by the particle as the particle passes through the light fringes using at least one light detector.

    Abstract translation: 根据一个实施例的系统包括用于产生光条纹的光源; 用于引导粒子通过光条纹的采样机构; 以及至少一个光检测器，用于当粒子通过光条纹时检测由粒子散射的光。 根据一个实施例的方法包括使用光源产生光条纹; 引导粒子通过光条纹; 以及当至少一个光检测器通过所述光条纹时，检测由所述粒子散射的光。

    Method and Apparatus for Measuring a Position of a Particle in a Flow
    9.
    发明申请
    Method and Apparatus for Measuring a Position of a Particle in a Flow 有权
    用于测量流中颗粒位置的方法和装置

    公开(公告)号：US20110051137A1

    公开(公告)日：2011-03-03

    申请号：US12851866

    申请日：2010-08-06

    Applicant: Thomas H. Jeys ,  Antonio Sanchez-Rubio ,  Ronald H. Hoffeld ,  Jonathan Z. Lin ,  Nicholas M.F. Judson ,  George S. Haldeman ,  Vincenzo Daneu

    Inventor： Thomas H. Jeys ,  Antonio Sanchez-Rubio ,  Ronald H. Hoffeld ,  Jonathan Z. Lin ,  Nicholas M.F. Judson ,  George S. Haldeman ,  Vincenzo Daneu

    IPC: G01N21/00 ,  G01B11/14

    CPC classification number: G01N21/53 ,  G01B11/002 ,  G01N15/1459 ,  G01N2015/145

    Abstract: Aerosol and hydrosol particle detection systems without knowledge of a location and velocity of a particle passing through a volume of space, are less efficient than if knowledge of the particle location is known.An embodiment of a particle position detection system capable of determining an exact location of a particle in a fluid stream is discussed. The detection system may employ a patterned illuminating beam, such that once a particle passes through the patterned illuminating beam, a light scattering is produced. The light scattering defines a temporal profile that contains measurement information indicative of an exact particle location.However, knowledge of the exact particle location has several advantages. These advantages include correction of systematic particle measurement errors due to variability of the particle position within the sample volume, targeting of particles based on position, capture of particles based on position, reduced system energy consumption and reduced system complexity.

    Abstract translation: 没有了解通过一定空间的颗粒的位置和速度的气溶胶和水溶胶颗粒检测系统比知道颗粒位置的知识效率低。 讨论了能够确定流体流中颗粒的确切位置的粒子位置检测系统的实施例。 检测系统可以使用图案化的照明光束，使得一旦粒子通过图案化的照明光束，就产生光散射。 光散射定义了包含指示精确粒子位置的测量信息的时间分布。 然而，确切的粒子位置的知识有几个优点。 这些优点包括校正由于样品体积内的颗粒位置的变异性引起的系统的颗粒测量误差，基于位置的颗粒靶向，基于位置的颗粒捕获，降低的系统能量消耗和降低的系统复杂性。

    METHOD AND APPARATUS FOR DETECTING AND DISCRIMINATING PARTICLES IN A FLUID
    10.
    发明申请
    METHOD AND APPARATUS FOR DETECTING AND DISCRIMINATING PARTICLES IN A FLUID 有权
    用于检测和鉴别流体中颗粒的方法和装置

    公开(公告)号：US20100053614A1

    公开(公告)日：2010-03-04

    申请号：US12029300

    申请日：2008-02-11

    Applicant: Thomas H. Jeys ,  Antonio Sanchez-Rubio ,  Richard J. Molnar ,  Robert K. Reich ,  Jinendra K. Ranka ,  David L. Spears ,  Richard M. Osgood, III

    Inventor： Thomas H. Jeys ,  Antonio Sanchez-Rubio ,  Richard J. Molnar ,  Robert K. Reich ,  Jinendra K. Ranka ,  David L. Spears ,  Richard M. Osgood, III

    IPC: G01N21/47 ,  G01N21/85

    CPC classification number: G01N15/1434 ,  G01N15/1425 ,  G01N15/1459 ,  G01N2015/025 ,  G01N2015/1075 ,  G01N2015/145

    Abstract: A particle detection system that images and detects particles in a fluid flow stream through use of detector array(s) is described. The detection system may include light source arrays that may selectively illuminate a particle in a fluid stream. The detection system may also include a detector array employing smart binning to read the measured signals. The smart binning of the detector array may be achieved through knowledge of an exact particle location provided by a position sensitive detector. The detector array(s) may be low cost based on intelligence built into the system. This particle detection system may be particularly useful for detection and discrimination of different particle types since the read-out of the particle signals can be accomplished with low noise and can be flexible enough to optimize the read out measurements for each particle. The particle detection system may be used, for example, in early warning contamination detection systems and manufacturing processes.

    Abstract translation: 描述了通过使用检测器阵列对流体流中的颗粒进行成像和检测的颗粒检测系统。 检测系统可以包括可以选择性地照射流体流中的颗粒的光源阵列。 检测系统还可以包括采用智能合并来读取测量信号的检测器阵列。 可以通过了解由位置敏感检测器提供的精确粒子位置来实现检测器阵列的智能合并。 基于内置于系统中的智能，检测器阵列可以是低成本的。 该粒子检测系统可能特别适用于不同粒子类型的检测和鉴别，因为可以以低噪声实现粒子信号的读出，并且可以足够灵活以优化每个粒子的读出测量。 粒子检测系统可以用于例如早期预警污染检测系统和制造过程。

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